Establishment and Characterization of Callus and Cell Suspension Cultures of Selected Sorghum bicolor (L.) Moench Varieties: A Resource for Gene Discovery in Plant Stress Biology

Ramulifho, Elelwani; Goche, Tatenda; As, Johann Van; Tsilo, Toi J.; Chivasa, Stephen; Ngara, Rudo

doi:10.3390/agronomy9050218

Cited by 25 publications

(18 citation statements)

References 49 publications

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“…ICSB338 sorghum ( Sorghum bicolor ) cell suspension cultures initiated from shoot derived, dark-grown callus [ 36 ] were used in this study. Although the heat phenotypic trait of ICSB338 is currently unknown, this sorghum variety is relatively more susceptible to salt [ 37 ] and drought [ 38 ] stresses compared to other sorghum varieties.…”

Section: Methodsmentioning

confidence: 99%

“…Cell cultures were grown and maintained under continuous darkness with agitation at 27 °C (±2 °C) every 10–12 days and sub-cultured for at least three generations prior to stress treatments. Eight-day old ICSB338 sorghum cell suspension culture, growing at mid-log phase [ 36 ] were used for the heat stress experiments. Triplicate 50 mL cell culture samples each for the control and heat stress treatments were generated.…”

Section: Methodsmentioning

confidence: 99%

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Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

et al. 2020

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Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes. Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

et al. 2020

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“…Seleksi kalus remah juga dilakukan pada SE karet terutama pada jalur long-term SE (Cailloux et al, 1996), kalus ini juga digunakan sebagai bahan penyimpanan kalus jangka panjang dengan kriopreservasi (Lardet et al, 2007). Seleksi kalus juga dilakukan pada kultur suspensi Artemisia Annua L. (Jin & Keng, 2013), Boerhaavia paniculata Rich (Souza et al, 2014), Manihot esculenta Crantz (Apio et al, 2015), Grewia tenax (Dafalla et al, 2019), Sorghum bicolor L. (Ramulifho et al, 2019). Kalus remah memiliki Kalus primer stevia berupa kalus dengan struktur lunak berwarna bening atau translusen.…”

Section: Proliferasi Dan Seleksi Kalusunclassified

Inisiasi, pertumbuhan, dan perkembangan kalus embriogenik tanaman stevia (Stevia rebaudiana)

Sinta¹,

Saptari²,

Sumaryono³

2021

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The leaves of sweetener plant Stevia rebaudiana contain secondary metabolites of steviol glycosides which are very sweet, with no calorie and zero glycemic index. Propagation of stevia by seeds is ineffective due to its low germination rate and diverse progenies. The tissue culture of stevia can be used to mass propagate rapidly and is commonly conducted by shoot multiplication. Up to now, the technology of somatic embryogenesis (SE) in stevia has not been successful yet. SE is developed to increase the production scale, rejuvenate clonal-propagated plants, and plant genetic transformation. The research objective was to develop protocols for the initiation, proliferation, and development of embryogenic calli of stevia as potential materials for SE. The explants used were young leaves, nodes, and internodes of axenic plantlets of stevia BX clone. The explants were cultured on MS solid media containing different concentrations of auxin and cytokinin for callus initiation. Callus emerged after 2-3 weeks of culture. The calli obtained were proliferated by subculturing several times as material stocks for indirect SE. MS solid media added with 1 µM 3,4-D and 16 mM CaCl2 gave the highest callus multiplication rate (4.7 times in 3 weeks). The selection of embryogenic calli was made continuously to obtain a pure line of embryogenic calli. Three types of calli attained were friable, fast-growing, yellowish calli, shiny nodular calli, and greenish nodular calli. Histological studies revealed that cells of the nodular calli had been differentiated to potentially formed somatic embryos.

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“…Also, it really depends on the level of endogenous auxin, both in the growth regulator organic plus liquitermy fertilizer and the organic pesticides my-1. The study of [26] showed that the callus process was highly dependent on explants and the addition of growth regulators to in vitro basic media. Based on the results of the coefficient of diversity test, the best porang embryogenic callus explants were seen in PortLT 3 (2ppm) because they were not significantly different from PortLT 2 (1ppm) and others.…”

Section: Porang Seed Embryogenic Callus Inductionmentioning

confidence: 99%

In vitro cultured and supply chain porang in South Sumatra

2021

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The objectives of the study are first, farmers in P4S Karya Tani can obtain superior porang seeds by regenerating porang in vitro culture through organic plus liquitermy fertilizer. Second, analyzing the media for growing tissue and the concentration of each porang plant media in P4S. Third, analysis of the supply chain structure of porang in P4S. This study uses quantitative and qualitative approaches with simple randomized design methods and SCOR methods. Based on the experiment, vitro culture of porang in P4S using liquitermy fertilizer, there were nineteen out of thirty porang plant accessions which had the potential as new clones for seedlings with an average tuber of 6.00 - 99.88% larger than the parent. The porang supply chain in P4S needs to be developed by involving wider stakeholders. This study shows that the seed production rate is better with liquitermy fertilizer in the porang in vitro culture. However, further evaluation is needed in analyzing genetic traits and glucomannan levels

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Establishment and Characterization of Callus and Cell Suspension Cultures of Selected Sorghum bicolor (L.) Moench Varieties: A Resource for Gene Discovery in Plant Stress Biology

Cited by 25 publications

References 49 publications

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Inisiasi, pertumbuhan, dan perkembangan kalus embriogenik tanaman stevia (Stevia rebaudiana)

In vitro cultured and supply chain porang in South Sumatra

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