Establishment and Characterization of an Epstein-Barr Virus Nuclear Antigen-Negative Hairy Cell Line

Abstract

Continuous cell lines have been established from spleen cells of patients with confirmed hairy cell leukemia (HCL). One cell line, HCL-Z1, lacks Epstein-Barr virus nuclear antigen (EBNA), grows attached to the substratum and retains typical features of hairy cells as revealed by transmission and scanning electron microscopy. HCL-Z1 differs morphologically from the three other EBNA-positive lymphoblastoid cell lines obtained (HCL-Z2, HCL-Z3, HCL-Z4) as well as from normal spleen cells or lymphocytes. The three lymphoblastoid cell lines derived from HCL patients show similar surface features as a line from a myeloma patient. Therefore, not all cell lines derived from HCL patients may be considered as representative of the patients leukemia cells.

Morphological Aspects of Interactions between Asbestos Fibers and Human Mesothelial Cell Cytoskeleton

“…Normal cultures and detergent-extracted cultures were washed with phosphate-buffered saline, pH 7.4, fixed in 2.5% glutaraldehyde in cacodylate buffer ( 1 h at 4 °C) and processed for SEM as described [Lang et al, 1985],…”