Establishment and Characterization of Acute B-Cell Lymphocytic Leukemia Cell Line Showing (8; 14) and (14; 18) Chromosome Translocation

Matsuzaki, Hiromitsu; Hata, Hiroyuki; Asou, Norio; Suzushima, Hitoshi; Akahoshi, Yoshitaka; Yoshida, Minoru; Nagakura, Shoichi; Ishii, Toshinorí; Sanada, Isao; Takatsuki, Kiyoshi

doi:10.1159/000205054

Cited by 21 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2b). This change is observed for both the L3͞Bcl-2 transfectant and KHM-2B, an unusual BL line carrying (in addition to the hallmark 8:14 myc-translocation) a 14:18 translocation juxtaposing bcl-2 to the IgH chain locus (18).…”

Section: Da Can Promote Apoptosis In Cycling B Cells While Sparing Rementioning

confidence: 90%

Dopamine targets cycling B cells independent of receptors/transporter for oxidative attack: Implications for non-Hodgkin’s lymphoma

Meredith

Holder

Rosén

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Human B lymphocytes and derived lines from a spectrum of B cell malignancy were studied for expression of dopaminergic pathway components and for their cytostatic response to the catecholamine and related, potentially therapeutic compounds. Proliferating normal lymphocytes and dividing malignant clones rapidly arrested on exposure to dopamine in the low (<10 M) micromolar range. The antiparkinsonian drugs L-DOPA and apomorphine (particularly) were similarly antiproliferative. With the exception of D4, dopamine receptors D1-D5 were variably expressed among normal and neoplastic B cell populations, as was the dopamine transporter. Transcripts for D1 and D2 were frequently found, whereas D3 and D5 revealed restricted expression; dopamine transporter was detected in most cases. Nevertheless, pharmacological analysis disclosed that dopamine targeted cycling B cells independent of these structures. Rather, oxidative stress constituted the primary mechanism: the catecholamine's actions being mimicked by hydrogen peroxide and reversed by exogenous catalase, and evidence for the intracellular redox protein thioredoxin contributing protection. Among proliferating clones, growth arrest was accompanied by cell death in populations deplete in antiapoptotic Bcl-2: resting lymphocytes escaping low micromolar dopamine toxicity. Dysregulated bcl-2 expression, although preventing oxidative-induced caspase-dependent apoptosis, by itself conferred only minor protection against dopamine cytostasis. The selective impact of dopamine on lymphocytes that are in active cycle indicates an axis for therapeutic intervention not only in B cell neoplasia but also in lymphoproliferative disturbances generally. Rational tailoring of drug delivery systems already in development for Parkinson's disease could provide ideal vehicles for carrying the oxidative hit directly to the target populations.apoptosis ͉ oxidative stress ͉ Parkinson's disease ͉ Bcl-2 ͉ monoamines

show abstract

Section: Da Can Promote Apoptosis In Cycling B Cells While Sparing Rementioning

confidence: 90%

Dopamine targets cycling B cells independent of receptors/transporter for oxidative attack: Implications for non-Hodgkin’s lymphoma

Meredith

Holder

Rosén

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…WILL2 was established from a patient with DLBCL showing surface IGλ and t(8;22)(q24;q11) in our laboratory . KHM10B and KHM2B were established from patients diagnosed with B‐cell type of acute lymphoblastic leukemia (FAB: L3) (purchased from JCRB, Osaka, Japan) . Both KHM10B and KHM2B are positive for surface IGλ.…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning of IGλ rearrangements using long‐distance inverse PCR (LDI‐PCR)

Shimanuki

Sawada

Hosoi

et al. 2012

European J of Haematology

View full text Add to dashboard Cite

show abstract

“…In the patient sample and KMS-12PE, 4.5- and 1.5-kb mRNAs for the PRAD1/Cyclin D1 gene were detected (arrows). Lane 1: 1 µg of the total RNA from patient’s peripheral blood mononuclear cells; lane 2: 0.5 µg of the total RNA from KMS-12PE (a myeloma cell line with translocation 11;14) [26] as a positive control; lane 3: 5 µg of the total RNA from KHM-2B (a B-acute lymphoblastic leukemia cell line with translocations 8;14 and 14;18) [27] as a negative control. b Ethidium-bromide stained gel.…”

Section: Resultsmentioning

confidence: 99%

A Plasma Cell Leukemia Patient Showing Bialleic 14q Translocations: t(2;14) and t(11;14)

Sawada

Matsuzaki²,

Satterwhite

et al. 1999

Acta Haematol

View full text Add to dashboard Cite

We report here an IgG/λ-type plasma cell leukemia patient showing bialleic 14q32 translocations. All immunoglobulins were suppressed in this patient, but a small amount of monoclonal IgG was detected by immunoelectrophoresis. Two cells of six peripheral blood mononuclear cells showed 46,XY,t(2;14)(q11;q32), i(8)(q10), t(11;14)(q13;q32), del(12)(q13.1) by karyotypic analysis. We confirmed the juxtaposition of IgH and PRAD1/Cyclin D1 genes by fluorescent in situ hybridization and overexpression of the PRAD1/Cyclin D1 gene, but Southern analysis showed no bcl-1 rearrangement. We analyzed the t(2;14)(q11;q32) using DNA fragments derived from childhood B-chronic lymphocytic leukemia cases bearing t(2;14)(p13;q32). Southern and Northern analyses demonstrated no alteration of these genes, indicating that this t(2;14) was different from that of childhood B-chronic lymphocytic leukemia. At the IgH loci, Southern analysis showed two rearranged bands and one germ-line band of JH. Cμ was deleted on one rearranged allele but remained on the other, suggesting that the chromosome translocation occurred after productive class switch recombination on the Cμ deleted allele.

show abstract

Establishment and Characterization of Acute B-Cell Lymphocytic Leukemia Cell Line Showing (8; 14) and (14; 18) Chromosome Translocation

Cited by 21 publications

References 18 publications

Dopamine targets cycling B cells independent of receptors/transporter for oxidative attack: Implications for non-Hodgkin’s lymphoma

Dopamine targets cycling B cells independent of receptors/transporter for oxidative attack: Implications for non-Hodgkin’s lymphoma

Molecular cloning of IGλ rearrangements using long‐distance inverse PCR (LDI‐PCR)

A Plasma Cell Leukemia Patient Showing Bialleic 14q Translocations: t(2;14) and t(11;14)

Contact Info

Product

Resources

About