Molecular cloning of IGλ rearrangements using long‐distance inverse PCR (LDI‐PCR)

Abstract

Our method is a useful tool for molecular analysis of genetic events in IGλ.

Ultrasensitive RNA In Situ Hybridization for Detection of Restricted Clonal Expression of Low-Abundance Immunoglobulin Light Chain mRNA in B-Cell Lymphoproliferative Disorders

“…The IGLL5 expression pattern may simply reflect the stage of B-cell maturity/development. 20,21 Indeed, we observed IGLL5 expression more often in DLBCL, as noted above. In rare NHL cases (an FL of WHO grade 3A ❚Image 8❚ and a biclonal DLBCL), cells with both k and l intranuclear signals were observed, but in each instance, the process was overtly clonally restricted.…”

PVT1: A Cancer-associated Non-coding Gene Revisited

“…Although few breakpoints within PVT1 have been cloned, some putative breakpoint clusters have been found near PVT1 exon 1, Burkitt's Variants' Rearranged Region 1 (BVR1 ), and the human homologous region of Pvt1 (Figure 1) [21][22][23][24]. However, the relationship between clinicopathological observations and these breakpoint clusters remains unknown.…”

IGLL5 controlled by super-enhancer affects cell survival and MYC expression in mature B-cell lymphoma

“…The frequently-encountered mutated genes seen in B-cell tumors, such as BCL2 or EZH2 , have some functions in B-cell malignancies. In addition, IGLL5 has also been shown to be a target of chromosome translocation in mature-B-cell lymphoma or multiple myeloma [ 10 , 11 ]. Generally, a target gene of chromosome translocation in hematopoietic tumors plays important roles in the tumorigenesis.…”