Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene

Kujiraoka, Satoko; Takemoto, Tsunematsu; Sato, Yukiko; Yoshida, Maki; Ishikawa, Ayataka; Tohyama, Rei; Tanaka, Michio; Kobayashi, Yutaka; Kondo, Takao; Ushio, Aya; Otsuka, Kunihiro; Kurosawa, Mie; Saitō, Masako; Yamada, Akiko; Arakaki, Rieko; Nagai, Hirokazu; Nikai, Hiromasa; Takeuchi, Kengo; Nagao, Toshitaka; Miyamoto, Youji; Ishimaru, Naozumi; Kudo, Yasusei

doi:10.1016/j.oraloncology.2017.04.003

Cited by 23 publications

(17 citation statements)

References 29 publications

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“…This study showed the correlation between ATF1 and lung cancer, suggesting that ATF1 may play important roles in lung tumor progression. In several reports, EWSR1‐ATF1 fusion genes were found in some cancer cells . Thus, EWSR1 rearrangements and fusion with the ATF1 gene are a hallmark for tumor diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2

Cui

Yin

et al. 2019

Molecular Carcinogenesis

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Lung cancer is among the most frequently occurring cancers and the leading cause of cancer‐related deaths worldwide. Nonsmall cell lung cancer is accountable for 85% to 90% of all lung cancer cases and develops distant metastases with high mortality. In this work, we elucidated the role of activating transcription factor 1 (ATF1) in migration and invasion of lung cancer cells. We found that the migration and invasion were inhibited with ATF1 silencing in lung cancer cells. By contrast, ATF1 overexpression led to promotion in migration and invasion. The alteration in ATF1 expression induced a change in the epidermal growth factor receptor (EGFR) and matrix metalloproteinases (MMP)‐2 expression level in the same tendency. Thus, we provided a potential new candidate for therapies against lung cancer, showing the possible mechanism underlying the invasion and migration of lung cancer cells.

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Section: Discussionmentioning

confidence: 99%

Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2

Cui

Yin

et al. 2019

Molecular Carcinogenesis

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“…To date, several studies 1,[11][12][13][14][15][16] have described the genetics of CCOC. Seventeen CCOC specimens have been tested for Ewing sarcoma breakpoint region 1 (EWSR1) translocations using either fluorescence in situ hybridization (FISH) 1,[14][15][16] or real-time polymerase chain reaction (RT-PCR) and FISH analysis.…”

Section: Statement Of Clinical Relevancementioning

confidence: 99%

“…Seventeen CCOC specimens have been tested for Ewing sarcoma breakpoint region 1 (EWSR1) translocations using either fluorescence in situ hybridization (FISH) 1,[14][15][16] or real-time polymerase chain reaction (RT-PCR) and FISH analysis. 12 Genetic analysis showed that EWSR1 was partnered with cyclic AMP-dependent transcription factor (ATF1) in 7 samples, cAMP response element-binding protein (CREB) in 1 sample, and recently, cAMP responsive element modulator (CREM) in 1 sample. 1 The remainder of the samples had an unknown rearrangement location and/ or partner.…”

Section: Statement Of Clinical Relevancementioning

confidence: 99%

Detection of EWSR1 fusions in CCOC by targeted RNA-seq

Rivera

Faquin

Thierauf

et al. 2022

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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“…The identification and validation of therapeutic targets for patients with head and neck tumours are limited due to a lack of a suitable ex vivo models. In this sense, establishment of animal models of xenotransplantation can facilitate the studies of therapeutic approaches and tumourigenesis in diverse tumours (Hidalgo et al, ; Kujiraoka et al, ; Li et al, ; Oakes et al, ; Pearson et al, ; Peng et al, ; Siolas & Hannon, ; Zhou et al, ). In the present study, we aimed to evaluate the possibility of AFD tumour growth in a patient‐derived xenograft (PDX) model.…”

mentioning

confidence: 99%

“…NUDE mice do not usually reject transplants and have therefore been widely used as a model for the investigation of neoplasms, including head and neck carcinomas (Pearson et al, ; Priolo et al, ; Sousa, Garcia, Nogueira, Furtado, & Anjos, ; Yokota et al, ; Zhou et al, ). Additionally, the PDX has an important applicability in translational studies, since it allows a better elucidation of carcinogenesis pathways and a better therapeutic evaluation (Hidalgo et al, ; Kujiraoka et al, ; Li et al, ; Oakes et al, ; Pearson et al, ; Peng et al, ; Siolas & Hannon, ; Zhou et al, ). However, xenotransplantation has not been used in benign odontogenic tumours yet.…”

mentioning

confidence: 99%

Patient‐derived xenografts of a case of ameloblastic fibrodentinoma

et al. 2019

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Objectives The establishment of animal models of xenotransplantation can contribute to the elucidation of the molecular pathogenesis of ameloblastic fibrodentinomas (AFD) and it also provides an opportunity for drug tests. We aimed to evaluate the possibility of AFD tumour growth in a patient‐derived xenograft (PDX) model. In addition, we characterized the human tumour and the PDXs. Materials and Methods A sample of a recurrent AFD was obtained and two fragments were contralaterally implanted subcutaneously in an 8‐week old female NUDE mouse. After 250 days, the PDXs were removed and submitted to histopathological and molecular analysis. Immunohistochemical reactions for Ki67 and the phosphorylated form of ERK1/2 were carried out in both, PDXs and human tumour, and the presence of BRAFV600E was assessed. Results From day 135 onwards, the PDXs presented a growth peak and remained stable until day 250. Histopathologically, the PDXs presented the same features of the patient’s tumour. Tumour cells exhibited Ki67 and pERK1/2 immunoexpression in the patient’s tumour and PDX. The AFD was wild‐type for BRAFV600E. Conclusion The PDX model recapitulated well the human tumour after a long implantation time, representing a possible model to study the AFD and other odontogenic tumours pathobiology.

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Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene

Cited by 23 publications

References 29 publications

Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2

Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2

Detection of EWSR1 fusions in CCOC by targeted RNA-seq

Patient‐derived xenografts of a case of ameloblastic fibrodentinoma

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