Establishment and characterization of a primary androgen‐responsive African‐American prostate cancer cell line, E006AA

Koochekpour, Shahriar; Maresh, Grace A.; Katner, Adrienne; Parker-Johnson, KiTani A.; Lee, Tae Jin; Hébert, Francine; Kao, Yuan; Skinner, John M.; Rayford, Walter

doi:10.1002/pros.20053

Cited by 53 publications

(79 citation statements)

References 31 publications

(47 reference statements)

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“…The presence of a marker chromosome involving in chromosome 4 (M1=del(4)(q28q34)+hsr in some) in both cell lines has been reported in other African American derived metastatic prostate cancer cell line (MDA PCa) and a primary African American prostate cancer cell line, E006AA (3,4). However, the observation of a common karyotypic pattern (regional deletion of 13q, 5q, 16q and 8p and gains of 8q and 5q) of primary prostate cancer derived from African American men and Caucasian American men led to the conclusion that biological interracial differences among the prostate cancer patients are not due to gross chromosomal alterations (15).…”

Section: Discussionmentioning

confidence: 77%

“…), M4=der(22?). To date only two models (MDA PCa and E006AA) of African American prostate cancer-derived human prostate cancer cell lines exist (3,4). MDA PCa cell lines were derived from a single bone metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…To determine the effects of androgen stimulation on the growth of RC-77N/E and RC-77T/E cells, 2x10 4 cells per well were grown in serum-free K-SFM with 0.1% BSA in the presence of 0, 0.1, 1.0, 10.0 and 100.0 nM for 4 days. K-SFM was supplemented with or without Methyltrienolone (R1881) (Perkin-Elmer, Waltham, MA), at concentrations of 0.1, 1, 10 and 100 nM, respectively.…”

Section: Generation Of Rc-77t/e and Rc-77n/e Cell Linesmentioning

confidence: 99%

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Establishment and characterization of a pair of non-malignant and malignant tumor derived cell lines from an African American prostate cancer patient

Theodore

Sharp²,

Zhou³

et al. 2010

Int J Oncol

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Abstract.Research into molecular and genetic mechanisms underlying prostate carcinogenesis in high-risk African American men would be greatly advanced by in vitro models of African American prostate tumors representing primary tumors. However, the generation of immortalized primary African American prostate cancer cells that will accurately reflect the in situ characteristics of malignant epithelium is currently limited but is greatly needed. We have successfully established immortalized cell lines of a pair of non-malignant and malignant tumors derived from an African American prostate cancer patient with HPV-16E6E7 (RC-77N/E and RC-77T/E). RC-77N/E and RC-77T/E cells are currently growing well at passage 40. Both cells exhibit epithelial morphology and are androgen sensitive. The RC-77T/E cells produced tumors in SCID mice whereas the RC-77N/E cells produced no tumor in SCID mice. These cells expressed androgen-regulated prostate-specific homobox gene, NKX 3.1, epithelial cell specific cytokeratn 8, androgen receptor (AR), prostate specific antigen (PSA), and p16. Chromosome analysis showed that both cell lines are similar; near diploid human male (XY) with most chromosome counts in the 45-48 range. However, RC-77T/E cell line has new marker chromosomes: M1B=del/t(4;?)(q28;?), M5=16q+ in addition to those observed in the RC-77N/E cell line (M1=del(4)(q28q34)+hsr in some, M1A=t(4q;?),M2=der(9?),M2A=del(M2p-),M3=iso(?), M4=der(22?)). This is the first documented case of the establishment of pair of non-malignant and malignant tumors derived from an African American prostate cancer patient. These models will provide novel tools to study the molecular and genetic mechanisms of prostate carcinogenesis, especially for high-risk African American men.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Generation Of Rc-77t/e and Rc-77n/e Cell Linesmentioning

confidence: 99%

See 1 more Smart Citation

Establishment and characterization of a pair of non-malignant and malignant tumor derived cell lines from an African American prostate cancer patient

Theodore

Sharp²,

Zhou³

et al. 2010

Int J Oncol

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“…E006AA is one of the few cell lines established from primary PCa and originates from an African-American patient with hormone naïve localized PCa (Table 2; Koochekpour et al 2004). A stromal cell line (S006AA) established in parallel from the same patient material further extends the experimental value of E006AA by enabling autologous epithelial-stromal interactions to be studied in vitro.…”

Section: Cell Lines Established Directly From Pca Patient Tissuesmentioning

confidence: 99%

In vitro model systems to study androgen receptor signaling in prostate cancer

Sampson

Neuwirt

Puhr

et al. 2013

Endocrine-Related Cancer

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Prostate cancer (PCa) is one of the most common causes of male cancer-related death in Western nations. The cellular response to androgens is mediated via the androgen receptor (AR), a ligand-inducible transcription factor whose dysregulation plays a key role during PCa development and progression following androgen deprivation therapy, the current mainstay systemic treatment for advanced PCa. Thus, a better understanding of AR signaling and new strategies to abrogate AR activity are essential for improved therapeutic intervention. Consequently, a large number of experimental cell culture models have been established to facilitate in vitro investigations into the role of AR signaling in PCa development and progression. These different model systems mimic distinct stages of this heterogeneous disease and exhibit differences with respect to AR expression/status and androgen responsiveness. Technological advances have facilitated the development of in vitro systems that more closely reflect the physiological setting, for example via the use of three-dimensional coculture to study the interaction of prostate epithelial cells with the stroma, endothelium, immune system and tissue matrix environment. This review provides an overview of the most commonly used in vitro cell models currently available to study AR signaling with particular focus on their use in addressing key questions relating to the development and progression of PCa. It is hoped that the continued development of in vitro models will provide more biologically relevant platforms for mechanistic studies, drug discovery and design ensuring a more rapid transfer of knowledge from the laboratory to the clinic.

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“…Our efforts to determine whether sensitivity of AAE varies among cells that express high levels of AR but are mitogenically unresponsive to androgens (e.g. CW22RV1 and E006AA) (39,44) showed no consistent pattern. The AAE was significantly less potent in these two cells when compared with that of LNCaP cells.…”

Section: Discussionmentioning

confidence: 99%

Ericifolin: a novel antitumor compound from allspice that silences androgen receptor in prostate cancer

Shamaladevi

Lyn²,

Shaaban

et al. 2013

Carcinogenesis

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Establishment and characterization of a primary androgen‐responsive African‐American prostate cancer cell line, E006AA

Cited by 53 publications

References 31 publications

Establishment and characterization of a pair of non-malignant and malignant tumor derived cell lines from an African American prostate cancer patient

Establishment and characterization of a pair of non-malignant and malignant tumor derived cell lines from an African American prostate cancer patient

In vitro model systems to study androgen receptor signaling in prostate cancer

Ericifolin: a novel antitumor compound from allspice that silences androgen receptor in prostate cancer

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