Establishment and biological characteristics of fibroblast cell lines obtained from wild corsac fox

Li, Xihe; Li, Yunxia; Yan, Xiao‐Jie; Guo, Xiaonan; Song, Yongli; Wu, Baojiang; Bao, Siqin; Cao, Guifang; Guo, Jitong; Sun, Qing‐Yuan

doi:10.1007/s11626-020-00527-5

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…After the Hi-C corrected contigs were placed in the ALLhic pipeline for segmentation, orientation, and sequencing, the final 99.49% of the assembled sequences were anchored to 18 pseudochromosomes with chromosome lengths ranging from 64.55 Mb to 202.44 Mb (Table S6, Figure 1B). This result is in agreement with the karyotyping results, which are based on cytological observations [21]. Following Hi-C correction, the final genome assembly was 2.2 Gb, scaffold N50 was 132.2 Mb, and contig N50 was 41.62 Mb (Table S7).…”

Section: Genome Sequencing and Assemblysupporting

confidence: 89%

High-Quality Chromosome-Level Genome Assembly of the Corsac Fox (Vulpes corsac) Reveals Adaptation to Semiarid and Harsh Environments

Zhang

Xia

Zhou

et al. 2023

IJMS

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The Corsac fox (Vulpes corsac) is a species of fox distributed in the arid prairie regions of Central and Northern Asia, with distinct adaptations to dry environments. Here, we applied Oxford-Nanopore sequencing and a chromosome structure capture technique to assemble the first Corsac fox genome, which was then assembled into chromosome fragments. The genome assembly has a total length of 2.2 Gb with a contig N50 of 41.62 Mb and a scaffold N50 of 132.2 Mb over 18 pseudo-chromosomal scaffolds. The genome contained approximately 32.67% of repeat sequences. A total of 20,511 protein-coding genes were predicted, of which 88.9% were functionally annotated. Phylogenetic analyses indicated a close relation to the Red fox (Vulpes vulpes) with an estimated divergence time of ~3.7 million years ago (MYA). We performed separate enrichment analyses of species-unique genes, the expanded and contracted gene families, and positively selected genes. The results suggest an enrichment of pathways related to protein synthesis and response and an evolutionary mechanism by which cells respond to protein denaturation in response to heat stress. The enrichment of pathways related to lipid and glucose metabolism, potentially preventing stress from dehydration, and positive selection of genes related to vision, as well as stress responses in harsh environments, may reveal adaptive evolutionary mechanisms in the Corsac fox under harsh drought conditions. Additional detection of positive selection for genes associated with gustatory receptors may reveal a unique desert diet strategy for the species. This high-quality genome provides a valuable resource for studying mammalian drought adaptation and evolution in the genus Vulpes.

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Section: Genome Sequencing and Assemblysupporting

confidence: 89%

High-Quality Chromosome-Level Genome Assembly of the Corsac Fox (Vulpes corsac) Reveals Adaptation to Semiarid and Harsh Environments

Zhang

Xia

Zhou

et al. 2023

IJMS

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“…In addition, the transcriptomic change induced by MMC may be also related to reduced cell numbers and/or density of the cultures. This is supported by previous studies with 3T3 and primary fibroblasts showing that cell growth, and/or increased cell-cell contacts due to higher cell density, can be linked to regulation of gene expression or protein levels [63][64][65][66][67][68][69][70][71] . In addition, altered cell morphology, that may also occur in response to higher cell density, can result in distinct gene expression 72 .…”

Section: Discussionsupporting

confidence: 78%

Macromolecular crowding regulates matrix composition and gene expression in human gingival fibroblast cultures

Ramalingam

Jiang

Larjava

et al. 2023

Sci Rep

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Standard cell cultures are performed in aqueous media with a low macromolecule concentration compared to tissue microenvironment. In macromolecular crowding (MMC) experiments, synthetic polymeric crowders are added into cell culture media to better mimic macromolecule concentrations found in vivo. However, their effect on cultured cells is incompletely understood and appears context-dependent. Here we show using human gingival fibroblasts, a cell type associated with fast and scarless wound healing, that MMC (standard medium supplemented with Ficoll 70/400) potently modulates fibroblast phenotype and extracellular matrix (ECM) composition compared to standard culture media (nMMC) over time. MMC significantly reduced cell numbers, but increased accumulation of collagen I, cellular fibronectin, and tenascin C, while suppressing level of SPARC (Secreted Protein Acidic and Cysteine Rich). Out of the 75 wound healing and ECM related genes studied, MMC significantly modulated expression of 25 genes compared to nMMC condition. MMC also suppressed myofibroblast markers and promoted deposition of basement membrane molecules collagen IV, laminin 1, and expression of LAMB3 (Laminin Subunit Beta 3) gene. In cell-derived matrices produced by a novel decellularization protocol, the altered molecular composition of MMC matrices was replicated. Thus, MMC may improve cell culture models for research and provide novel approaches for regenerative therapy.

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“…Hence, the establishment of primary elephant dermal fibroblast (EDF) cell lines would allow for the acquisition of large amounts of cells with most of their original characteristics preserved for functional and genetic analyses [10]. Primary fibroblast cell lines have previously been established from several endangered animal species in captivity such as collared peccaries (Pecari tajacu) [11], jaguars (Panthera onca) [12], and a wild corsac fox (Vulpes corsac) [13]. They have also been established from fresh carcasses of a common hippopotamus calf (Hippopotamus amphibius) [14], a Chinese muntjac (Muntiacus reevesi) [15], a Sumatran rhinoceros (Dicerorhinus sumatrensis) [16] and from Asian elephants (Elephas maximus) [17], to name a few examples.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (Loxodonta africana)

Bolcaen

Engelbrecht

Burger³

et al. 2023

Animals

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Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.

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Establishment and biological characteristics of fibroblast cell lines obtained from wild corsac fox

Cited by 7 publications

References 10 publications

High-Quality Chromosome-Level Genome Assembly of the Corsac Fox (Vulpes corsac) Reveals Adaptation to Semiarid and Harsh Environments

High-Quality Chromosome-Level Genome Assembly of the Corsac Fox (Vulpes corsac) Reveals Adaptation to Semiarid and Harsh Environments

Macromolecular crowding regulates matrix composition and gene expression in human gingival fibroblast cultures

Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (Loxodonta africana)

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