2013
DOI: 10.1590/s0102-09352013000400044
|View full text |Cite
|
Sign up to set email alerts
|

Establishing the hypoosmotic swelling test for sperm analysis in collared peccaries (Pecari tajacu)

Abstract: Communication [Comunicação] Establishing the hypoosmotic swelling test for sperm analysis in collared peccaries (Pecari tajacu)[Estabelecimento do teste hiposmótico para a análise de sêmen de catetos (Pecari tajacu)]

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
13
1

Year Published

2015
2015
2024
2024

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 22 publications
(14 citation statements)
references
References 10 publications
(13 reference statements)
0
13
1
Order By: Relevance
“…Assessment of the plasma membrane functional integrity was performed by the hyposmotic swelling test (HOST) by adding 10µL of semen to 90µL distilled water (0 mOsmol/L) followed by immersion in dry bath at 37°C for 40 min (Santos et al 2013). After incubation, reading of percentage of HOST reactive sperm was performed under optical microscopy (Olympus  CX 21), using immersion objective (obj.1000x) in wet mount between slide and coverslip stained with 3% rose bengal.…”
Section: Methodsmentioning
confidence: 99%
“…Assessment of the plasma membrane functional integrity was performed by the hyposmotic swelling test (HOST) by adding 10µL of semen to 90µL distilled water (0 mOsmol/L) followed by immersion in dry bath at 37°C for 40 min (Santos et al 2013). After incubation, reading of percentage of HOST reactive sperm was performed under optical microscopy (Olympus  CX 21), using immersion objective (obj.1000x) in wet mount between slide and coverslip stained with 3% rose bengal.…”
Section: Methodsmentioning
confidence: 99%
“…The functional integrity of the sperm membrane was verified by evaluating the sperm osmotic response under a hypo‐osmotic swelling test, using distilled water (0 mOsm/L) as the hypo‐osmotic solution. A total of 200 cells were examined, and those with a swollen coiled tail were considered as presenting a functional membrane (Santos et al, ).…”
Section: Methodsmentioning
confidence: 99%
“…Functional membrane integrity was evaluated as the sperm osmotic response to a hypoosmotic swelling (HOS) test, using distilled water (0mOsm/l). Two hundred sperm were examined and those with a swollen, coiled tail were considered to have a functional membrane (Santos et al ., 2013). Sperm viability was determined by bromophenol blue stained smears, using light microscopy (×400 magnification), based on analyses of 200 cells per slide (Sousa et al , 2013).…”
Section: Methodsmentioning
confidence: 99%