Establishing errors in the spectrophotometric determination of chlorophylls using different solvents

García, Antonio Pérez; Nicolás, N.

doi:10.1016/s0176-1617(98)80253-8

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…Seedlings from both treatments were watered daily with 150 to 300 mL of nutrient solution for three weeks, depending on plant growth rate. The following variables were then determined: chlorophyll a (chla) and chlorophyll b (chlb) content, chla/chlb ratio, and surface chlorophyll 854 A. L. Garcia et al / Span J Agric Res (2011) 9(3), 852-861 density (total chlorophyll/area ratio), according to García and Nicolás (1998). Growth variables and leaf mineral composition were determined as indicated above.…”

Section: Methodsmentioning

confidence: 99%

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

García

Madrid

Gimeno

et al. 1970

Span. j. agric. res.

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The aim of this research was to determine the effects on growth variables and leaf mineral concentration of tomato plants watered with nutrient solutions containing amino acids. Two separate experiments were then carried out to achieve this goal. In the first experiment, plants were watered with seven different nutrient solutions consisting of half-strength Hoagland solution supplemented with single (Alanine, Serine, Phenylalanine, Tyrosine) or combined (Ala + Ser; Phe + Tyr) amino acids, each at 0.2 mM of concentration. The control nutrient solution did not have any amino acids added. Relative to the control, growth variables were not affected by the presence of amino acids. In general, the mixture of Ala + Ser increased the leaf Ca 2+ concentration, and the aliphatic amino acid treatments favoured an increase in leaf K + , Fe, Cu, and Mn concentrations. In addition, amino acids with hydroxyl groups in their structure, Ser and Tyr, increased Mg 2+ concentration. In the second experiment, the nutrient solutions were supplemented with 0.05 mM of the same amino acids (T1). Control plants were irrigated with amino acid-free nutrient solution (T0). The concentration of Ca 2+ , K + , Mg 2+ , Fe, Cu, and Mn in the leaf also increased due the amino acids treatment. Chlorophyll contents in the leaves and amino acids compositions in the xylem sap and leaf water relation were also determined. In conclusion, the data reported in the two experiments point out that the application of amino acids to the nutrient solution has a beneficial effect on the leaf mineral status and on the chlorophyll concentration of the leaves.Additional key words: amino acid fertilization; biofertilizer; hydroponic culture; Solanum lycopersicum L. ResumenEfectos de la aplicación de amino ácidos en la solución nutritiva sobre la composición mineral y el crecimiento de plántulas de tomate En este trabajo se estudiaron los efectos sobre el crecimiento y la concentración mineral de hojas de plantas de tomate cultivadas con soluciones nutritivas con distinta composición en aminoácidos. Para ello se plantearon dos experimentos. En el primero las plantas se regaron con siete soluciones nutritivas 1/2 Hoagland que diferían en la composición de los aminoácidos, todos ellos a una concentración total de 0,2 mM (Ala, Ser, Fen y Tir aplicados de forma individual, dos con la combinación Ala+Ser y Fen+Tir, un control sin aminoácidos). Las variables de crecimiento no mostraron diferencias entre los tratamientos; sin embargo, se observaron cambios en las concentraciones de nutrientes aunque los efectos dependieron de los aminoácidos utilizados. La mezcla de Ala + Ser aumentó la concentración de Ca 2+ , mientras que los tratamientos de aminoácidos alifáticos favorecieron un aumento de K + , Fe, Cu y Mn, y aquellos con grupos hidroxilo (Ser y Tyr) aumentaron el Mg 2+ . En el segundo experimento las plantas se regaron con disolución 1/2 Hoagland (T0) o con disolución 1/2 Hoagland más una mezcla de los 4 aminoácidos (0,05 mM) ensayados (T1). En este experiment...

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Section: Methodsmentioning

confidence: 99%

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

García

Madrid

Gimeno

et al. 1970

Span. j. agric. res.

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“…The spectrophotometric absorbance properties of the Chlorophyll molecules facilitate their qualitative and quantitative analysis using different solvents, and the contribution of these solvents to the extraction in various species was compared [21]. The extraction efficiency of chlorophyll is closely related to the type of organic solvent [22], [23] due to the differences in the leaf tissue [24] and anatomic characteristics. However, the results of the fractional study are the result of humidity and pH treatment close to the extreme, so it is difficult to directly compare them with other plants.…”

Section: Resultsmentioning

confidence: 99%

Effect of Chlorophyll in Garlic Chives (Allium tuberosum) Leaves by Drought and pH

Huh

2022

EJFOOD

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Allium tuberosum Rotter (garlic chives) is a species of plant native to the Chinese province and naturalized elsewhere in Asia and around the world. In Korea, chives are boiled and used as seasoned vegetables or as soup ingredients, and are also used for chive kimchi and cucumber pickles. This paper describes experimental results for chlorophyll content and efficiency with drought stress and soil acidity on garlic chives. Analytical determination of total chlorophyll (a, b) was performed with spectrophotometer. Chlorophyll fluorescence analysis was used to measure photosynthetic parameters with the Imaging-PAM Chlorophyll Fluorometer. The content of total chlorophyll was 0.711 at 80% moisture. The content for chlorophylls a, b, and total were 0.333, 0.151, and 0.390 at 10% moisture, respectively. The simple type of regression showed the relation between chlorophylls as a function of acidity. The slope factors for chlorophyll content (a, b, and total) were 0.866, 0.903, and 0.910, respectively. The current fluorescence yield (Ft) was increased to the decrease of water in soils. The maximal PSII quantum yield, Fv/Fm was the highest at 70% moisture among nine moisture percentage stages. The slope factors of Ft, Fm (maximum fluorescence yield), Fv (quenched state), and Fv/Fm for chlorophyll efficient indicators were -0.873, 0.977, 969, and 0.897, respectively. Water stress decreased chlorophyll a, chl b, chl (a + b) contents, and the chl a/b ratio in the A. tuberosum. Low soil pH also affected the chlorophyll content and efficiency of A. tuberosum.

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Using DMSO for chlorophyll spectroscopy

2021

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Establishing errors in the spectrophotometric determination of chlorophylls using different solvents

Cited by 7 publications

References 16 publications

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings

Effect of Chlorophyll in Garlic Chives (Allium tuberosum) Leaves by Drought and pH

Using DMSO for chlorophyll spectroscopy

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