Essential oil of Mitracarpus frigidus as a potent source of bioactive compounds

Abstract

In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 μg/mL). On the other hand, it showed a strong antifungal effect against Cryptoccocus neoformans (MIC 8 μg/mL) and Candida albicans (MIC 63 μg/mL). Expressive activity against L. major and L. amazonensis promastigote forms with IC 50 values of 47.2 and 89.7 μg/mL, respectively, were also observed. In addition, the antioxidant activity was investigated through DPPH radical-scavenging and showed a significative activity with IC 50 of 38 μg/mL. The cytotoxicity against Artemia salina was moderate with LC 50 of 88 μg/mL. The results presented here are the first report on the chemical composition and biological properties of M. frigidus essential oil.

Effect of essential oils on Leishmania amazonensis: a systematic review

“…Mitracarpus frigidus : Chemical analysis of the M. frigidus EO obtained by hydrodistillation of the aerial parts of the plant resulted in the identification of 12 known compounds, linalool (29.29%) and eugenol acetate (15.85%) were the major constituents, followed by 5-hydroxy-isobornyl isobutyrate (8.41%), 5-methyl-1-undecene (7.69%) and methyl salicylate (6.55%) (Fabri et al ., 2012). This EO exhibited a potent antifungal effect against Cryptoccocus neoformans , Candida albicans and an expressive activity against L. amazonensis promastigote forms.…”

Versatile and Synergistic Potential of Eugenol: A Review

“…About 30 % of eugenol treated promastigotes and amastigotes were found to contain two or more flagella or nuclei indicating the arrest of cell division [51]. It showed anti-leishmanial activity against L. major promastigote with IC 50 value of 47.2 µg/ml [52]. Methanolic extract of Piper betle containing eugenol exhibited anti-leishmanial potential against Leishmania donovani [53].…”

Antileishmanial activity of some Brazilian plants, with particular reference to Casearia sylvestris

“…Due to this, a different sensitivity of theses parasites to the tested extracts was expected. Previous in vitro studies have also shown differences in sensitivity of Leishmania species to different reference drugs, including pentavalent antimonials, amphotericin B (Minodier and Parola 2007), miltefosine (De Morais-Teixeira et al 2011) and crude or purified plant extracts (Braga et al 2007, Fabri et al 2012a. Among the 17 methanolic extracts tested, 11 showed leishmanicidal activity against at least one promastigote forms of Leishmania species with IC 50 values ranging from 5.0 to 88.3 µg/mL.…”