Essential Oil of Ocimum basilicum L. and (−)‐Linalool Blocks the Excitability of Rat Sciatic Nerve

Abstract

The racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb) derived from Germplasm Bank rich in (−)-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (−)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP). EOOb and (−)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38 ± 0.2 and 0.17 ± 0.0 mg/mL, respectively. For (−)-LIN, these values were 0.23 ± 0.0 and 0.13 ± 0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (−)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−)-LIN in the essential oil.

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

“…Basil and its essential oil are often used in traditional medicine (Irondi et al 2016;Ezeani et al 2017;Bae et al 2020), cosmetics (Vivas Castaño et al 2016;Volpe et al 2018;Yeşil et al 2020), and pharmacology (Sestili et al 2018;Zhan et al 2020). Among the pharmacologically interesting compounds found in basil are linalool (Medeiros Venancio et al 2016), rosmarinic (Kwon et al 2019), oleanolic (OA; Qamar et al 2020) and ursolic acid (UA; Arshad Qamar et al 2010;Kümmritz et al 2014). The latter are triterpenoids, a class of isoprenoid-based secondary metabolites.…”

Discriminative stimulus properties of Cannabis sativa terpenes in rats

“…Terpenes represent one such class of compounds in cannabis that until recently have not been thoroughly investigated for potential therapeutic effects (Russo, 2011). While a growing body of literature suggests that terpenes might offer beneficial effects ranging from antinociception (Peana et al ., 2006; Batista et al ., 2008, 2010; Gertsch et al ., 2008; Berliocchi et al ., 2009; Kuwahata et al ., 2013; Nascimento et al ., 2014; Piccinelli et al ., 2015, 2017; Medeiros Venancio et al ., 2016; Araujo-Filho et al ., 2017; Segat et al ., 2017; Aguilar-Avila et al ., 2019; Aly et al ., 2019; Abbasijahromi et al ., 2020; LaVigne et al ., 2021; Bilbrey et al ., 2022), to anxiolytic properties (Linck et al ., 2010; Souto-Maior et al ., 2011; Bahi et al ., 2014; Harada et al ., 2018; Abbasijahromi et al ., 2020), the pharmacological mechanisms of these purported effects remain poorly characterized (see Russo, 2011 for a summary of the proposed pharmacological effects of terpenes). Two recent reports suggested that CB1 receptors might be one target for a number of terpenes present in cannabis (LaVigne et al ., 2021; Bilbrey et al ., 2022) as evidenced by cannabimimetic activity in the tetrad assay.…”

<p>Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line</p>

“…1 In the past few years, the majority of research in this field has focused on novel approaches for delivering drugs, depending on the drug-delivery system. 2,3 Cancer is defined as a disorder in which extraordinary cells are amplified by escaping the traditional regulations of cellular division. Ovarian cancer SKOV-3 cells are a primary type of cancer cells.…”