Essential oil of Marrubium cuneatum Russell and its secretory elements

Abstract

The structure of the glandular trichomes and essential oil of the aerial parts of Marrubium cuneatum Russell were studied. Most secretory tissues are located in glandular trichomes. The essential oil isolated by steam distillation were analysed by GC-MS. Among 25 compounds identified, representing about 89% of the oil, the major components were bicyclogermacrene (37.9%) and germacrene D (24.1% Isolation of the Essential Oil and Preparing SlidesThe essential oil was obtained by water steam distillation of air-dried aerial parts (250 g) in an all-glass apparatus. The sample oil, which was light yellow in colour, was dried over anhydrous sodium sulphate and stored under nitrogen in a sealed vial until required. Fresh stems and leaves were placed in a formalin-acetic acid-ethyl alcohol mixture and slides were prepared by hand-cutting and stained in a alum carmine-methyl green combination (all of the chemicals used were from Merck). They were photographed at standard magnification using an Olympus automatic camera. Gas Chromatography-Mass SpectrometryGC-MS analysis was carried out on a Varian 3400 GC-MS system equipped with a DB-I fused silica column (60 m × 0.25 mm, film thickness 0.25 µm) and interfaced with a Varian ion trap detector. Oven temperature, 50-270°C at a rate of 4°C/min; injector and transfer line temperature, 280°C and 290°C; carrier gas, helium with a linear velocity of 31.5 cm/s; split ratio, 1:60; ionization energy, 70 eV; scan time, 1 s; mass range, 40-400 amu.

Comparison of chemical composition of the essential oil from Marrubium vulgare L. and M. incanum Desr. during the second year of cultivation

“…The herb of M. peregrinum L. contained 0.07% of essential oil, that of M. parviflorum Fisch & C.A. Mey -0.08% [16], while in M. cuneatum Russell -up to 0.15% [17].…”

“…Germacrene D was the prevailing component in essential oils from other species of the genus Marrubium sp. : M. cuneatum Russell [17], M. parviflorum Fisch & C.A. Mey -0.08% [16], and M. thessalum [21].…”

Composition and Antimicrobial Activity of Marrubium Incanum Desr. (Lamiaceae) Essential Oil

“…(E)-Caryophyllene, bicyclogermacrene and germacrene D were also the main constituents of the oils from M. peregrinum L. originating from Slovakia [11] and M. parviflorum Fisch. from Iran [12], whereas bicyclogermacrene and germacrene D were the most dominant in M. cuneatum Russell from Iran [13]. High amounts of (E)-caryophyllene and germacrene D were detected in the oils of M. vulgare [11,12], together with β-bisabolene and (E)-β-farnesene in the oil from Iran [12].…”

“…In official medicine, however, only M. vulgare is recognized, mostly for its beneficial effects for dyspeptic complaints and loss of appetite [8,9]. In the literature there are reports on the essential oil composition of some Marrubium species [11][12][13][14]. Sesquiterpenes constitute the main fraction of most Marrubium oils.…”

Chemical profile of the volatile compounds from Marrubium cuneatum Banks & Sol.

“…In Turkey an infusion that prepared from the leaves of M. cuneatum were used to treat abdominal pain (Tetik et al, 2013). The only reports on the essential oils, from plants collected from Iran and Lebanon have been published (Baher Nik et al, 2004;Grassia et al, 2006).…”