Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature

Nebesářová, Jana

doi:10.1007/978-1-4939-9469-4_4

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The SEM samples were prepared according to Nebesářová’s method [ 38 ]. Root samples 1 cm from rhizome were collected in +Tr4 CF and +Tr4 AFBCF treatments 60 days after inoculation.…”

Section: Methodsmentioning

confidence: 99%

Synchronized Efficacy and Mechanism of Alkaline Fertilizer and Biocontrol Fungi for Fusariumoxysporum f. sp. cubense Tropical Race 4

Jiang

et al. 2022

JoF

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The purpose of this study was to determine the effect and mechanism of alkaline fertilizer, bio-control fungi, and their synergistic application on control of Fusarium Tr4 incidence. Synchronized use of the alkaline fertilizer and biocontrol fungi eliminates rhizome browning and reduces the incidence rate of banana Fusarium wilt. The incidence of yellow leaves (ratio of yellow leaf to total leaf) and disease index in +Foc Tr4 CF treatment were the same (65%), while incidence of yellow leaves and disease index in +Foc Tr4 AFBCF were 31% and 33%, respectively. Under the stress of Foc Tr4 infection, the synergistic utilization of the alkaline fertilizer and biocontrol fungi would raise the activities of peroxidase, catalase and superoxide dismutase in banana roots. The root activity of banana was also increased. As a result, the banana height and stem diameter increments, shoot and root dry weight, accumulation of N, P and K in banana plants had been increased. The efficacy of the synergistic application of alkaline fertilizer and biocontrol fungi was not only reducing Foc Tr4 pathogen colonization and distribution in banana plants, but also preventing tylosis formation in vascular vessel effectively. Therefore, the normal transport of water and nutrients between underground and aboveground is ensured.

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“…The SEM samples were prepared according to Nebesářová’s method [ 38 ]. Root samples 1 cm from rhizome were collected in +Tr4 CF and +Tr4 AFBCF treatments 60 days after inoculation.…”

Section: Methodsmentioning

confidence: 99%

Synchronized Efficacy and Mechanism of Alkaline Fertilizer and Biocontrol Fungi for Fusariumoxysporum f. sp. cubense Tropical Race 4

Jiang

et al. 2022

JoF

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“…Subsequently, the deionized water was removed and samples were eluted with 70%, 85%, 95%, and 100% ethanol, respectively. Finally, the sample were glued on stubs, covered with a thin conductive layer and observed by SEM after critical-point drying and gold sputtering [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris

Xiao

Wang

Liu

et al. 2021

JoF

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Hydrophobins are a family of small proteins exclusively secreted by fungi, and play a variety of roles in the life cycle. Cmhyd1, one of the hydrophobin class II members in Cordyceps militaris, has been shown to have a high transcript level during fruiting body development. Here, deletion of Cmhyd1 results in reduction in aerial mycelia, conidiation, hydrophobicity and infection ability, and complete inhibition of pigmentation and primordium differentiation. Cmhyd1 plays roles in conidiation and cuticle-bypassing infection by regulating the transcripts of frequency clock protein, Cmfrq, and velvet protein, Cmvosa, as well as primordium formation via the mitogen-activated protein kinase signaling pathway. Cmhyd1 also participates in stress response, including tolerance of mycelia to osmotic and oxidative stresses, and conidia to high or low temperatures. CmAreA, a transcription factor of nitrogen regulatory, is recruited to the promoter of Cmhyd1 and activates the transcription of Cmhyd1 with coactivator CmOTam using electrophoretic mobility shift assays and transient luciferase expression in tobacco. Furthermore, CmHYD1 is proved to regulate the transcription of Cmarea at different developmental stages via a positive feedback loop. These results reveal the diverse roles and regulation of Cmhyd1 in C. militaris, and provide insights into the developmental regulatory mechanism of mushrooms.

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Comparison and development of scanning electron microscope techniques for delicate plant tissues

Yuan,

Yang,

et al. 2024

Plant Science

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Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature

Cited by 5 publications

References 19 publications

Synchronized Efficacy and Mechanism of Alkaline Fertilizer and Biocontrol Fungi for Fusariumoxysporum f. sp. cubense Tropical Race 4

Synchronized Efficacy and Mechanism of Alkaline Fertilizer and Biocontrol Fungi for Fusariumoxysporum f. sp. cubense Tropical Race 4

Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris

Comparison and development of scanning electron microscope techniques for delicate plant tissues

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