The TF cell line, derived from a carnivorous marine teleost, the turbot (Scophthalmus maximus), is known to have a reduced rate of polyunsaturated fatty acid (PUFA) biosynthesis.In order to establish the enzymic step responsible, the metabolic conversions of a range of PUFA including the key intermediates of n-3PUFA metabolism, stearidonic acid (18:4n-3) and its elongation product 20:4n-3, were studied in TF cells and compared with the AS cell line derived from Atlantic salmon (Salmo salar). Cells were cultured in the presence of unlabelled (25 µM 3