Espécies E/Ou Linhagens De Trichogramma Spp. (Hymenoptera: Trichogrammatidae) Para O Controle De Anticarsia Gemmatalis (Lepidoptera: Noctuidae)

Vianna, Ulysses Rodrigues; Pratissoli, Dirceu; Zanúncio, José Cola; Alencar, J. R.C.C. de; Zinger, Fernando Domingo

doi:10.1590/1808-1657v78p0812011

Cited by 11 publications

(1 citation statement)

References 31 publications

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“…The velvetbean caterpillar, Anticarsia gemmatalis (AG) Hübner (Lepidoptera: Noctuidae) is one of the primary defoliating pests in the Americas, affecting mainly soybean crops, and a major cause of economic losses in agriculture [ 51 , 52 ]. In recent years, alternative approaches towards pest control, such as the development of biopesticides, have been explored.…”

Section: Utility and Discussionmentioning

confidence: 99%

Propedia: a database for protein–peptide identification based on a hybrid clustering algorithm

et al. 2021

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Background Protein–peptide interactions play a fundamental role in a wide variety of biological processes, such as cell signaling, regulatory networks, immune responses, and enzyme inhibition. Peptides are characterized by low toxicity and small interface areas; therefore, they are good targets for therapeutic strategies, rational drug planning and protein inhibition. Approximately 10% of the ethical pharmaceutical market is protein/peptide-based. Furthermore, it is estimated that 40% of protein interactions are mediated by peptides. Despite the fast increase in the volume of biological data, particularly on sequences and structures, there remains a lack of broad and comprehensive protein–peptide databases and tools that allow the retrieval, characterization and understanding of protein–peptide recognition and consequently support peptide design. Results We introduce Propedia, a comprehensive and up-to-date database with a web interface that permits clustering, searching and visualizing of protein–peptide complexes according to varied criteria. Propedia comprises over 19,000 high-resolution structures from the Protein Data Bank including structural and sequence information from protein–peptide complexes. The main advantage of Propedia over other peptide databases is that it allows a more comprehensive analysis of similarity and redundancy. It was constructed based on a hybrid clustering algorithm that compares and groups peptides by sequences, interface structures and binding sites. Propedia is available through a graphical, user-friendly and functional interface where users can retrieve, and analyze complexes and download each search data set. We performed case studies and verified that the utility of Propedia scores to rank promissing interacting peptides. In a study involving predicting peptides to inhibit SARS-CoV-2 main protease, we showed that Propedia scores related to similarity between different peptide complexes with SARS-CoV-2 main protease are in agreement with molecular dynamics free energy calculation. Conclusions Propedia is a database and tool to support structure-based rational design of peptides for special purposes. Protein–peptide interactions can be useful to predict, classifying and scoring complexes or for designing new molecules as well. Propedia is up-to-date as a ready-to-use webserver with a friendly and resourceful interface and is available at: https://bioinfo.dcc.ufmg.br/propedia

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Section: Utility and Discussionmentioning

confidence: 99%

Propedia: a database for protein–peptide identification based on a hybrid clustering algorithm

et al. 2021

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Intestinal proteolytic profile changes during larval development of Anticarsia gemmatalis caterpillars

Junior

Vital

Barros

et al. 2019

Arch Insect Biochem Physiol

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Soybean is one of most consumed and produced grains in the world, and Anticarsia gemmatalis is a pest that causes great damage to this crop due to severe defoliation during its larval phase. Plants have mechanisms that lead to the inhibition of proteases in the intestine of these herbivores, hampering their development. Understanding this complex protease inhibitor is important for pest control. The objective of this study was to evaluate the enzymatic profiles of the intestinal proteases of the soybean caterpillar at different instars. For this, the proteolytic profile of the gut in the third, fourth, and fifth instars were analyzed.Irreversible inhibitors of proteases were separately incubated with A. gemmatalis enzyme extracts at the third, fourth, and fifth instar to assess the contribution of these proteases to total proteolytic activity. The enzymatic extracts were also evaluated with specific substrates to confirm changes in the specific activities of trypsin-like, chymotrypsin-like, and cysteine proteases at different instars. The results showed that the protease profile of A.gemmatalis gut changes throughout its larval development.The activity of cysteine proteases was more intense in the first instar. On the contrary, the serine proteases The protease profile of the gut of A. gemmatalis changes throughout larval development. In the third instar, cysteine protease activity is predominant over others. In the fourth and fifth instars, proteolytic activity is mainly due to serine proteases. This difference in activity in the different instars of A. gemmatalis suggests that the expression of the genes coding for proteases also changes throughout the development of the insect. Using serine PIs is a good strategy for controlling insect pests, as these are the main proteolytic enzymes of Lepidoptera. However, the presence of other important protease subclasses in insect digestibility suggests that a combination of serine and cysteine PIs may be promising, aiming to compromise larval development at different instars.

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Selection of Strains of Trichogramma foersteri and Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) for the Control of Palpita forficifera (Lepidoptera: Crambidae)

et al. 2022

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Espécies E/Ou Linhagens De Trichogramma Spp. (Hymenoptera: Trichogrammatidae) Para O Controle De Anticarsia Gemmatalis (Lepidoptera: Noctuidae)

Cited by 11 publications

References 31 publications

Propedia: a database for protein–peptide identification based on a hybrid clustering algorithm

Propedia: a database for protein–peptide identification based on a hybrid clustering algorithm

Intestinal proteolytic profile changes during larval development of Anticarsia gemmatalis caterpillars

Selection of Strains of Trichogramma foersteri and Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) for the Control of Palpita forficifera (Lepidoptera: Crambidae)

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