Escherichia coli ST502 and Klebsiella pneumoniae ST11 sharing an IncW plasmid harbouring the blaKPC-2 gene in an Intensive Care Unit patient

Almeida, Anna; Cavalcanti, Felipe Lira de Sá; Vilela, Marinalda Anselmo; Gales, Ana Cristina; Morais, Marcos Antônio de; Morais, Márcia Maria Camargo de

doi:10.1016/j.ijantimicag.2012.05.022

Cited by 17 publications

(12 citation statements)

References 5 publications

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“…Recently, there was reported the presence at the same hospital of a 65-kb IncW-type plasmid carrying bla KPC in Tn4401c among Enterobacteriaceae species (6). In the present study, we report a 65-kb IncW-type plasmid carrying bla KPC in Tn4401b in a K. oxytoca isolate, highlighting the diversity of mobile genetic elements related to the bla KPC gene in this institution and the high variability of the genetic hosts of this gene.…”

supporting

confidence: 60%

First Description of KPC-2-Producing Klebsiella oxytoca in Brazil

Almeida

Cavalcanti

Martins

et al. 2013

Antimicrob Agents Chemother

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The present work reports the detection of the first case of nosocomial Klebsiella oxytoca producing class A carbapenemase KPC-2 in Brazil. The isolate KPN106 carried a 65-kb IncW-type plasmid that harbors the bla KPC gene and Tn4401b. Moreover, we detected the presence of a class 1 integron containing a new allele, arr-8, followed by a 5=-truncated dhfrIIIc gene. In view of the recent results, we emphasize the high variability of the bacterial and genetic hosts of this resistance determinant.

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supporting

confidence: 60%

First Description of KPC-2-Producing Klebsiella oxytoca in Brazil

Almeida

Cavalcanti

Martins

et al. 2013

Antimicrob Agents Chemother

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“…However, different platforms also support bla KPC worldwide (34,38,39), including in Brazil (19,27). Tn4401 variants "a" and "c" were related (19,40), and the "b" variant has been described as predominant in K. pneumoniae (41) and in other genera and species (19,42) from Brazil. The Tn4401 variant "a" has been detected since 2007 (19) in hospitals and seems to be the single genetic environment/variant that supports bla KPC-2 in this health care center.…”

Section: Discussionmentioning

confidence: 99%

Expansion and Evolution of a Virulent, Extensively Drug-Resistant (Polymyxin B-Resistant), QnrS1-, CTX-M-2-, and KPC-2-Producing Klebsiella pneumoniae ST11 International High-Risk Clone

et al. 2014

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bIn this study, we report the early expansion, evolution, and characterization of a multiresistant Klebsiella pneumoniae clone that was isolated with increasing frequency from inpatients in a tertiary-care university hospital in Brazil. Seven carbapenem-and quinolone-resistant and polymyxin B-susceptible or -resistant K. pneumoniae isolates isolated between December 2012 and February 2013 were investigated. Beta-lactamase-and plasmid-mediated quinolone resistance (PMQR)-encoding genes and the genetic environment were investigated using PCR, sequencing, and restriction fragment length polymorphism (RFLP). Clonal relatedness was established using XbaI-pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and phylogenetic group characterization. Plasmid analyses included PCR-based replicon typing (PBRT) and hybridization of the S1-PFGE product, plasmid MLST, and conjugation experiments. Virulence potential was assessed by PCR by searching for 10 virulence factor-encoding genes (ureA, fimH, kfuBC, uge, wabG, magA, mrkD, allS, rmpA, and cf29a) and by phenotypic tests to analyze the hypermucoviscous phenotype. The genetic context of a multidrug-resistant and extensively drug-resistant K. pneumoniae ST11-KpI clone harboring IncFIIk-Tn4401a-bla KPC-2 , qnrS1, and bla CTX-M-2 was found. Moreover, three isolates displayed high resistance to polymyxin B (MICs ‫؍‬ 32, 32, and 128 mg/liter) as well as mucous and hypermucoviscous phenotypes. These bacteria also harbored ureA, fimH, uge, wabG, and mrkD, which code for virulence factors associated with binding, biofilm formation, and the ability to colonize and escape from phagocytosis. Our study describes the association of important coresistance and virulence factors in the K. pneumoniae ST11 international high-risk clone, which makes this pathogen successful at infections and points to the quick expansion and evolution of this multiresistant and virulent clone, leading to a pandrug-resistant phenotype and persistent bacteria in a Brazilian hospital.

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“…The integron in pSa is one of the earliest examples to carry ISCR1 (57). There are reports of detection (by PCR) of W plasmids carrying related cassette arrays containing bla VIM-1/4 (283,284) or bla KPC (285) in Tn4401, but sequences are not available. X plasmids.…”

Section: Resistance Plasmids In the Enterobacteriaceaementioning

confidence: 99%

Mobile Genetic Elements Associated with Antimicrobial Resistance

et al. 2018

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SUMMARYStrains of bacteria resistant to antibiotics, particularly those that are multiresistant, are an increasing major health care problem around the world. It is now abundantly clear that both Gram-negative and Gram-positive bacteria are able to meet the evolutionary challenge of combating antimicrobial chemotherapy, often by acquiring preexisting resistance determinants from the bacterial gene pool. This is achieved through the concerted activities of mobile genetic elements able to move within or between DNA molecules, which include insertion sequences, transposons, and gene cassettes/integrons, and those that are able to transfer between bacterial cells, such as plasmids and integrative conjugative elements. Together these elements play a central role in facilitating horizontal genetic exchange and therefore promote the acquisition and spread of resistance genes. This review aims to outline the characteristics of the major types of mobile genetic elements involved in acquisition and spread of antibiotic resistance in both Gram-negative and Gram-positive bacteria, focusing on the so-called ESKAPEE group of organisms (, ,, ,, spp., and), which have become the most problematic hospital pathogens.

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Escherichia coli ST502 and Klebsiella pneumoniae ST11 sharing an IncW plasmid harbouring the blaKPC-2 gene in an Intensive Care Unit patient

Cited by 17 publications

References 5 publications

First Description of KPC-2-Producing Klebsiella oxytoca in Brazil

First Description of KPC-2-Producing Klebsiella oxytoca in Brazil

Expansion and Evolution of a Virulent, Extensively Drug-Resistant (Polymyxin B-Resistant), QnrS1-, CTX-M-2-, and KPC-2-Producing Klebsiella pneumoniae ST11 International High-Risk Clone

Mobile Genetic Elements Associated with Antimicrobial Resistance

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