Escherichia coli heat-stable toxin receptors in human colonic tumors

Carrithers, Stephen L.; Parkinson, Scott J.; Goldstein, S.; Park, Pauline; Robertson, D C; Waldman, Scott A.

doi:10.1016/0016-5085(94)90804-4

Cited by 79 publications

(130 citation statements)

References 43 publications

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“…Tissues and blood were obtained under an Institutional Review Board-approved protocol from Thomas Jefferson University Hospital, the National Disease Research Interchange (Philadelphia), and the Cooperative Human Tissue Network (Philadelphia) (19,20). More than 280 human tissue samples were examined for ST binding and activation of guanylyl cyclase activity, and more than 260 tissue samples were examined for expression of GCC by reverse transcription-PCR (RT-PCR).…”

Section: Methodsmentioning

confidence: 99%

“…Membranes were prepared from tissues as described (19,20). ST was iodinated ( 125 I-labeled Tyr-4-ST) to a final specific activity of Ϸ2000 Ci͞mmol (1 Ci ϭ 37 GBq; ref.…”

Section: Methodsmentioning

confidence: 99%

“…22). Quantification of STreceptor binding and activation of guanylyl cyclase were performed as described (19,20,23). Assays were performed at least in duplicate and the intraexperimental variability was Յ12% (SEM).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, ST binding activity and guanylyl cyclase activation was identified in human primary and metastatic colorectal tumors (19,20). Thus, GCC may represent a selective marker of colorectal tumor metastases in extraintestinal tissues.…”

mentioning

confidence: 99%

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Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues

Carrithers

Barber

Biswas

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

181

189

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Guanylyl cyclase C (GCC) has been detected only in intestinal mucosa and colon carcinoma cells of placental mammals. However, this receptor has been identified in several tissues in marsupials, and its expression has been suggested in tissues other than intestine in placental mammals. Selective expression of GCC by colorectal tumor cells in extraintestinal tissues would permit this receptor to be employed as a selective marker for metastatic disease. Thus, expression of GCC was examined in human tissues and tumors, correlating receptor function with detection by PCR. GCC was detected by ligand binding and catalytic activation in normal intestine and primary and metastatic colorectal tumors, but not in extraintestinal tissues or tumors. Similarly, PCR yielded GCC-specific amplification products with specimens from normal intestine and primary and metastatic colorectal tumors, but not from extraintestinal tissues or tumors. Northern blot analysis employing GCC-specific probes revealed an Ϸ4-kb transcript, corresponding to recombinant GCC, in normal intestine and primary and metastatic colorectal tumors, but not in extraintestinal tissues. Thus, GCC is selectively expressed in intestine and colorectal tumors in humans and appears to be a relatively specific marker for metastatic cancer cells in normal tissues. Indeed, PCR of GCC detected tumor cells in blood from some patients with Dukes B colorectal cancer and all patients examined with Dukes C and D colorectal cancer, but not in that from normal subjects or patients with Dukes A colon carcinoma or other nonmalignant intestinal pathologies.

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Section: Methodsmentioning

confidence: 99%

“…Membranes were prepared from tissues as described (19,20). ST was iodinated ( 125 I-labeled Tyr-4-ST) to a final specific activity of Ϸ2000 Ci͞mmol (1 Ci ϭ 37 GBq; ref.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues

Carrithers

Barber

Biswas

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

181

189

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“…Mechanistically, STa binds to guanylyl cyclase-C (GC-C) receptors specifically expressed in intestinal cells (6). Ligand binding to GC-C activates the intracellular synthesis of the second messenger, cGMP.…”

mentioning

confidence: 99%

Diarrhea or colorectal cancer: Can bacterial toxins serve as a treatment for colon cancer?

Carrithers

2003

Proc. Natl. Acad. Sci. U.S.A.

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Molecular staging individualizing cancer management

Mejia

Schulz

Hyslop

et al. 2012

Journal of Surgical Oncology

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Although the most important prognostic and predictive marker in colorectal cancer is tumor cells in lymph nodes, ~30% of patients who are node-negative die from occult metastases. Molecular staging employing specific markers and sensitive detection technologies has emerged as a powerful platform to assess prognosis in node-negative colon cancer. Integrating molecular staging into algorithms that individualize patient management will require validation and the definition of relationships between occult tumor cells, prognosis, and responses to chemotherapy.

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Escherichia coli heat-stable toxin receptors in human colonic tumors

Cited by 79 publications

References 43 publications

Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues

Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues

Diarrhea or colorectal cancer: Can bacterial toxins serve as a treatment for colon cancer?

Molecular staging individualizing cancer management

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