Escherichia coli EAST1 toxin toxicity of variants 17-2 and O 42

Veilleux, Sophie; Holt, Natalee; Schultz, Bruce D.; Dubreuil, J. Daniel

doi:10.1016/j.cimid.2007.10.003

Cited by 20 publications

(16 citation statements)

References 19 publications

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“…Exposure of fully differentiated T84 cell monolayers mounted in Ussing flux chambers to purified enteroaggregative heat-stable toxin 1 (EAST1) results in an increase in the short-circuit current, a sensitive measure of net ion transport (646). The Pet toxin secreted by EAEC belongs to the SPATE family of toxins (306,506).…”

Section: Structural and Functional Injuriesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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Section: Structural and Functional Injuriesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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“…Comparing toxicity of purified 17-2 and O-42 EAST1 in the suckling mouse assay (CD-1 mice), a gut-to-body (G/B) weight ratio over 0.083 (which is considered toxic) was not observed for any of the EAST1 preparations tested beside the STa positive control [229]. Exposure of T84 cell monolayers to pure toxins in a modified Ussing flux chambers resulted in a rapidly attained and prolonged increase in short circuit current, a sensitive measure of net ion transport.…”

Section: East1 Toxicitymentioning

confidence: 93%

Escherichia coli heat-stable enterotoxins

Dubreuil

2015

The Comprehensive Sourcebook of Bacterial Protein Toxins

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“…Recombinant EAST1 from the strain 042 induces an increase in short circuit current in Ussing chambers indicative of a net anion secretion. 14 We compared DNA sequences through alignments of astA in Mandera's EASTEC strains with astA of the EAEC strain 042 which has been deposited in the GenBank (accession no. : FN554767).…”

Section: Epidemiologymentioning

confidence: 99%

“…Most investigators define a positive response as a G/B ratio of 0.083 or greater. 12,14,26 The culture filtrate of E. coli JM109 and sterile culture medium (TSB) were used as negative controls (G/B ratio of ≤ 0.059 and ≤ 0.058, respectively). CF from EASTEC outbreak strain (0081) was inoculated into suckling mice and gave a G/B ratio of 0.104 ± 0.004 (N = 6) ( Table 2).…”

Section: Epidemiologymentioning

confidence: 99%

“…However, the pathogenicity of EASTEC only has not been determined in an animal model and therefore the role of EAST1 in diarrhea remains an open question. [12][13][14] The objectives of this study were to identify the source of the diarrheal outbreak in Mandera, Kenya, and to suggest recommendations to prevent the occurrence of similar outbreaks in the future.…”

Section: Introductionmentioning

confidence: 99%

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An Outbreak of Diarrhea in Mandera, Kenya, Due to Escherichia coli Serogroup O-Nontypable Strain That Had a Coding Gene for Enteroaggregative E. coli Heat-Stable Enterotoxin 1

Ochi

Shah²,

Odoyo³

et al. 2017

The American Society of Tropical Medicine and Hygiene

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Abstract. In an outbreak of gastroenteritis in December 2009, in Mandera, Kenya, Escherichia coli O-nontypable (ONT) strain was isolated from stool specimens of patients (18/24, 75%). The E. coli ONT organisms could not be assigned to any of the recognized diarrheagenic groups of E. coli. However, they possessed the enteroaggregative E. coli heat-stable enterotoxin-1 gene. The cell-free culture filtrates of the E. coli ONT strain isolated from the outbreak cases induced considerable amount of fluid accumulation in suckling mouse intestine, indicating production of an enterotoxic factor(s). These results identify E. coli that did not have any diarrheagenic characteristics except astA as the etiological agent of the diarrheal outbreak in Mandera. It is however considered necessary to characterize the fluid accumulation factor(s) to determine whether any novel toxins were responsible for the fluid accumulation. Moreover, it is important to study dissemination of strains producing the enterotoxic factor(s) to assess their public health significance distribution in the environment.

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Escherichia coli EAST1 toxin toxicity of variants 17-2 and O 42

Cited by 20 publications

References 19 publications

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Escherichia coli heat-stable enterotoxins

An Outbreak of Diarrhea in Mandera, Kenya, Due to Escherichia coli Serogroup O-Nontypable Strain That Had a Coding Gene for Enteroaggregative E. coli Heat-Stable Enterotoxin 1

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