Escherichia coli Diversity in Livestock Manures and Agriculturally Impacted Stream Waters

Cook, Kyle DeMeo; Bolster, Carl H.; Ayers, Kati Anne; Reynolds, Dale N.

doi:10.1007/s00284-011-0002-6

Cited by 23 publications

(28 citation statements)

References 31 publications

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“…MLST-based phylogenetic analysis showed that plantassociated strains cover most of E. coli diversity, with the exception of the recently described phylogroup F. However, as observed in other environments (Walk et al, 2007;Orsi et al, 2007b;2007a;Ratajczak et al, 2010;Bergholz et al, 2011;Cook et al, 2011), we found an unequal phylogenetic distribution with a prevalence of B1 isolates, suggesting that the E. coli phylogroups are not equivalent in their ecology and that B1 may be different (O'Brien and Gordon, 2011). By combining phenotypic and phylogenetic characterization we could then reveal that phenotypes discriminating plant-and host-associated strains were phylogenetically distributed.…”

Section: Discussionmentioning

confidence: 91%

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Méric

Kemsley

Falush³

et al. 2012

Environmental Microbiology

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Plants are increasingly considered as secondary reservoirs for commensal and pathogenic Escherichia coli strains, but the ecological and functional factors involved in this association are not clear. To address this question, we undertook a comparative approach combining phenotypic and phylogenetic analyses of E. coli isolates from crops and mammalian hosts. Phenotypic profiling revealed significant differences according to the source of isolation. Notably, isolates from plants displayed higher biofilm and extracellular matrix production and higher frequency of utilization of sucrose and the aromatic compound p-hydroxyphenylacetic acid. However, when compared with mammalian-associated strains, they reached lower growth yields on many C-sources commonly used by E. coli. Strikingly, we observed a strong association between phenotypes and E. coli phylogenetic groups. Strains belonging to phylogroup B1 were more likely to harbour traits indicative of a higher ability to colonize plants, whereas phylogroup A and B2 isolates displayed phenotypes linked to an animal-associated lifestyle. This work provides clear indications that E. coli phylogroups are specifically affected by niche-specific selective pressures, and provides an explanation on why E. coli population structures vary in natural environments, implying that different lineages in E. coli have substantially different transmission ecology.

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Section: Discussionmentioning

confidence: 91%

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Méric

Kemsley

Falush³

et al. 2012

Environmental Microbiology

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“…For example, both hydrophobicity and zeta potential (as a measure of wetness) has been shown to be related to the growth rate or phase in E. coli (Allison et al, 1990; Smets et al, 1999). A comparison of 17 E. coli strains, isolated from livestock or water sources, showed an order of magnitude difference in attachment efficacy when binding to quartz sand, with the most efficient stains concurrently possessing the highest number of genes associated with adhesion, toxin production, iron acquisition, or capsular synthesis (Cook et al, 2011). The mineral chemical and surface composition, organic content and particle size affect the propensity of bacterial cells to adhere or release to the particles (Pachepsky et al, 2009b; Hazen and Sverjensky, 2010).…”

Section: Sediment Characteristics Governing Bacteria Particle Interacmentioning

confidence: 99%

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

et al. 2016

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The long term survival of fecal indicator organisms (FIOs) and human pathogenic microorganisms in sediments is important from a water quality, human health and ecological perspective. Typically, both bacteria and viruses strongly associate with particulate matter present in freshwater, estuarine and marine environments. This association tends to be stronger in finer textured sediments and is strongly influenced by the type and quantity of clay minerals and organic matter present. Binding to particle surfaces promotes the persistence of bacteria in the environment by offering physical and chemical protection from biotic and abiotic stresses. How bacterial and viral viability and pathogenicity is influenced by surface attachment requires further study. Typically, long-term association with surfaces including sediments induces bacteria to enter a viable-but-non-culturable (VBNC) state. Inherent methodological challenges of quantifying VBNC bacteria may lead to the frequent under-reporting of their abundance in sediments. The implications of this in a quantitative risk assessment context remain unclear. Similarly, sediments can harbor significant amounts of enteric viruses, however, the factors regulating their persistence remains poorly understood. Quantification of viruses in sediment remains problematic due to our poor ability to recover intact viral particles from sediment surfaces (typically <10%), our inability to distinguish between infective and damaged (non-infective) viral particles, aggregation of viral particles, and inhibition during qPCR. This suggests that the true viral titre in sediments may be being vastly underestimated. In turn, this is limiting our ability to understand the fate and transport of viruses in sediments. Model systems (e.g., human cell culture) are also lacking for some key viruses, preventing our ability to evaluate the infectivity of viruses recovered from sediments (e.g., norovirus). The release of particle-bound bacteria and viruses into the water column during sediment resuspension also represents a risk to water quality. In conclusion, our poor process level understanding of viral/bacterial-sediment interactions combined with methodological challenges is limiting the accurate source apportionment and quantitative microbial risk assessment for pathogenic organisms associated with sediments in aquatic environments.

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“…Therefore, improved understanding the variations of E. coli properties is needed for predicting fate and transport of the bacteria and to support the development of plans to reduce bacterial contamination of waters. Recent studies have indicated that there is high diversity of E. coli isolates in the environment (Lu et al, 2005; Bolster et al, 2009; Cook et al, 2011). This strain-level diversity has been described by differences in both genotype and phenotype, and therefore it likely impacts the fate and transport of E. coli .…”

Section: Introductionmentioning

confidence: 99%

E. coli Surface Properties Differ between Stream Water and Sediment Environments

et al. 2016

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The importance of E. coli as an indicator organism in fresh water has led to numerous studies focusing on cell properties and transport behavior. However, previous studies have been unable to assess if differences in E. coli cell surface properties and genomic variation are associated with different environmental habitats. In this study, we investigated the variation in characteristics of E. coli obtained from stream water and stream bottom sediments. Cell properties were measured for 77 genomically different E. coli strains (44 strains isolated from sediments and 33 strains isolated from water) under common stream conditions in the Upper Midwestern United States: pH 8.0, ionic strength 10 mM and 22°C. Measured cell properties include hydrophobicity, zeta potential, net charge, total acidity, and extracellular polymeric substance (EPS) composition. Our results indicate that stream sediment E. coli had significantly greater hydrophobicity, greater EPS protein content and EPS sugar content, less negative net charge, and higher point of zero charge than stream water E. coli. A significant positive correlation was observed between hydrophobicity and EPS protein for stream sediment E. coli but not for stream water E. coli. Additionally, E. coli surviving in the same habitat tended to have significantly larger (GTG)5 genome similarity. After accounting for the intrinsic impact from the genome, environmental habitat was determined to be a factor influencing some cell surface properties, such as hydrophobicity. The diversity of cell properties and its resulting impact on particle interactions should be considered for environmental fate and transport modeling of aquatic indicator organisms such as E. coli.

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Escherichia coli Diversity in Livestock Manures and Agriculturally Impacted Stream Waters

Cited by 23 publications

References 31 publications

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

E. coli Surface Properties Differ between Stream Water and Sediment Environments

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