E. coli Surface Properties Differ between Stream Water and Sediment Environments

Liang, Xiao; Liao, Chunyu; Thompson, Michael L.; Soupir, Michelle L.; Jarboe, Laura R.; Dixon, Philip M.

doi:10.3389/fmicb.2016.01732

Cited by 39 publications

(45 citation statements)

References 55 publications

(61 reference statements)

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“…Contrastingly, measurement of the cell surface hydrophobicity reflects the abundance and type of proteins and sugars on the cell surface, distinct from the membrane lipid distribution and associated fluidity (47). The environmental isolates used in this study are part of a larger set that was previously characterized in terms of various cell surface properties, including hydrophobicity (41). Hydrophobicity was found to range from 1 to 90% adhesion to hydrocarbons (41).…”

Section: Resultsmentioning

confidence: 99%

“…It has previously been demonstrated that short-chain fatty acids are inhibitory to E. coli, having a detrimental impact on both growth and fatty acid production (9,18,42). Several E. coli strains were previously isolated from Squaw Creek in Ames, IA, and characterized in terms of various cell surface properties and phylotype (Table 1) (41). Given that these environmental isolates have previously demonstrated an ability to survive and propagate under harsh environmental conditions, we proposed that these environmental isolates may be able to provide insight into design strategies for developing robust microbial cell factories for short-chain fatty acid production.…”

Section: Resultsmentioning

confidence: 99%

“…The environmental isolates used in this study are part of a larger set that was previously characterized in terms of various cell surface properties, including hydrophobicity (41). Hydrophobicity was found to range from 1 to 90% adhesion to hydrocarbons (41). Thus, in the current work, we also characterized the cell surface hydrophobicity during fatty acid challenge.…”

Section: Resultsmentioning

confidence: 99%

“…A previously described collection of environmental E. coli isolates was found to have wide variation in membrane properties, such as hydrophobicity and zeta potential (41). Here, we characterized these strains for their ability to tolerate and produce octanoic acid.…”

Section: Importancementioning

confidence: 99%

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Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates

Chen

Reinhardt

Neris

et al. 2018

Appl Environ Microbiol

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Fermentative production of many attractive biorenewable fuels and chemicals is limited by product toxicity in the form of damage to the microbial cell membrane. Metabolic engineering of the production organism can help mitigate this problem, but there is a need for identification and prioritization of the most effective engineering targets. Here, we use a set of previously characterized environmental isolates with high tolerance and production of octanoic acid, a model membrane-damaging biorenewable product, as a case study for identifying and prioritizing membrane engineering strategies. This characterization identified differences in the membrane lipid composition, fluidity, integrity, and cell surface hydrophobicity from those of the lab strain MG1655. Consistent with previous publications, decreased membrane fluidity was associated with increased fatty acid production ability. Maintenance of high membrane integrity or longer membrane lipids seemed to be of less importance than fluidity. Cell surface hydrophobicity was also directly associated with fatty acid production titers, with the strength of this association demonstrated by plasmid-based expression of the multiple stress resistance outer membrane protein BhsA. This expression of was effective in altering hydrophobicity, but the direction and magnitude of the change differed between strains. Thus, additional strategies are needed to reliably engineer cell surface hydrophobicity. This work demonstrates the ability of environmental microbiological studies to impact the metabolic engineering design-build-test-learn cycle and possibly increase the economic viability of fermentative bioprocesses. The production of bulk fuels and chemicals in a bio-based fermentation process requires high product titers. This is often difficult to achieve, because many of the target molecules damage the membrane of the microbial cell factory. Engineering the composition of the membrane in order to decrease its vulnerability to this damage has proven to be an effective strategy for improving bioproduction, but additional strategies and engineering targets are needed. Here, we studied a small set of environmental isolates that have higher production titers of octanoic acid, a model biorenewable chemical, than those of the lab strain MG1655. We found that membrane fluidity and cell surface hydrophobicity are strongly associated with improved octanoic acid production. Fewer genetic modification strategies have been demonstrated for tuning hydrophobicity relative to fluidity, leading to the conclusion that there is a need for expanding hydrophobicity engineering strategies in.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Importancementioning

confidence: 99%

See 2 more Smart Citations

Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates

Chen

Reinhardt

Neris

et al. 2018

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…These E. coli isolates were of different genotype according to our previous repetitive sequence based-PCR (rep-PCR) and fingerprint profile analysis, using a maximum similarity cut-off of 90% (Liang et al, 2016). The E. coli lab strain MG1655 was used as a K12 control.…”

Section: Methodsmentioning

confidence: 99%

Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

et al. 2017

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Understanding the genetic factors that govern microbe-sediment interactions in aquatic environments is important for water quality management and reduction of waterborne disease outbreaks. Although chemical properties of bacteria have been identified that contribute to initiation of attachment, the outer membrane proteins that contribute to these chemical properties still remain unclear. In this study we explored the attachment of 78 Escherichia coli environmental isolates to corn stover, a representative agricultural residue. Outer membrane proteome analysis led to the observation of amino acid variations, some of which had not been previously described, in outer membrane protein A (OmpA) at 10 distinct locations, including each of the four extracellular loops, three of the eight transmembrane segments, the proline-rich linker and the dimerization domain. Some of the polymorphisms within loops 1, 2, and 3 were found to significantly co-occur. Grouping of sequences according to the outer loop polymorphisms revealed five distinct patterns that each occur in at least 5% of our isolates. The two most common patterns, I and II, are encoded by 33.3 and 20.5% of these isolates and differ at each of the four loops. Statistically significant differences in attachment to corn stover were observed among isolates expressing different versions of OmpA and when different versions of OmpA were expressed in the same genetic background. Most notable was the increased corn stover attachment associated with a loop 3 sequence of SNFDGKN relative to the standard SNVYGKN sequence. These results provide further insight into the allelic variation of OmpA and implicate OmpA in contributing to attachment to corn stover.

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Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance

Jin

Rover

Petersen

et al. 2017

Journal of Industrial Microbiology and Biotechnology

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Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace compounds in this pyrolytic sugar syrup are inhibitory to microbial biocatalysts. This study demonstrates that hydrophobic inhibitors damage the cell membrane of ethanologenic Escherichia coli KO11+lgk. Adaptive evolution was employed to identify design strategies for improving pyrolytic sugar tolerance and utilization. Characterization of the resulting evolved strain indicates that increased resistance to the membrane-damaging effects of the pyrolytic sugars can be attributed to a glutamine to leucine mutation at position 29 of carbon storage regulator CsrA. This single amino acid change is sufficient for decreasing EPS protein production and increasing membrane integrity when exposed to pyrolytic sugars.

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E. coli Surface Properties Differ between Stream Water and Sediment Environments

Cited by 39 publications

References 55 publications

Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates

Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates

Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance

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