ESCHERICHIA COLI 0157 | Escherichia coli O157 and Other Shiga Toxin-Producing E. coli

Fratamico, Pina M.; Gehring, Andrew G.

doi:10.1016/b978-0-12-384730-0.00104-x

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…To detect Shiga toxin type-2 daily during milk production process up to low levels (0.01À1 pg/mL), immune PCR assays NFS 54,1 and sensitive ELISA were developed by using Stx2-1 and Stx2-2 monoclonal antibodies (He et al, 2013). On the other hand, detection of the same pathogen was achieved within 8 h through super-magnetic beads-based separation followed by ELISA or PCR (Fratamico and Gehring, 2014). Magnetosome-based biosensors have got attention for the detection of pathogens due to high efficiency and real-time deduction.…”

Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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Purpose Nature and occurrence of food-borne pathogens in raw and processed food products evolved greatly in the past few years due to new modes of transmission and resistance build-up against sundry micro-/macro-environmental conditions. Assurance of food health and safety thus gained immense importance, for which bio-sensing technology proved very promising in the detection and quantification of food-borne pathogens. Considering the importance, different studies have been performed, and different biosensors have been developed. This study aims to summarize the different biosensors used for the deduction of food-borne pathogens. Design/methodology/approach The present review highlights different biosensors developed apropos to food matrices, factors governing their selection, their potential and applicability. The paper discusses some related key challenges and constraints and also focuses on the needs and future research prospects in this field. Findings The shift in consumers’ and industries’ perceptions directed the further approach to achieve portable, user and environmental friendly biosensing techniques. Despite of these developments, it was still observed that the comparison among the different biosensors and their categories proved tedious on a single platform; since the food matrices tested, pathogen detected or diagnosed, time of detection, etc., varied greatly and very few products have been commercially launched. Conclusively, a challenge lies in front of food scientists and researchers to maintain pace and develop techniques for efficiently catering to the needs of the food industry. Research limitations/implications Biosensors deduction limit varied with the food matrix, type of organism, material of biosensors’ surface, etc. The food matrix itself consists of complex substances, and various types of food are available in nature. Considering the diversity of food there is a need to develop a universal biosensor that can be used for all the food matrices for a pathogen. Further research is needed to develop a pathogen-specific biosensor that can be used for all the food products that may have accuracy to eliminate the traditional method of deduction. Originality/value The present paper summarized and categorized the different types of biosensors developed for food-borne pathogens. Graphical abstract

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Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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“…The permissible levels of lead and cadmium in organic fertilizers, as mandated by the Sri Lanka Standard Institution and the European government, are summarized in Table 1. Escherichia coli is a rod-shaped, facultative anaerobic bacterium classified as gram-negative (Tenaillon et al 2010;Fratamico and Gehring 2014). E. coli is a diverse group of bacteria, and while some strains are harmless, others can cause serious illnesses.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the heavy metals and Escherichia coli contamination of liquid organic fertilizers in the local market, Sri Lanka

Yasara,

Dananjana,

Wijeratne

2024

Braz. J. Develop.

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Commercially available liquid organic fertilizers have gained popularity in agriculture due to their convenience and perceived environmental benefits. However, these fertilizers are susceptible to various contaminations, including heavy metals such as lead (Pb) and cadmium (Cd), as well as bacterial contaminants like Escherichia coli (E. coli). Monitoring these contaminants helps to identify potential health risks to consumers, prevent soil and water pollution, and maintain the quality of agricultural products. Therefore, this study involved a comprehensive analysis of Pb and Cd heavy metals and E. coli bacterial contaminants in two widely used liquid organic fertilizers in the local market. The samples were digested using an open-vessel acid digestion followed by the flame atomic absorption spectroscopic (FAAS) analysis. The limit of detection (LOD), limit of quantification (LOQ), matrix effect, and spike recovery were determined. The Pb concentration of one fertilizer was 3.45 (± 0.07) mg/kg with 92% recovery whereas the other was 2.87 (± 0.08) mg/kg with 91% recovery. In contrast, the Cd concentration was 0.09 (± 0.01) mg/kg and 0.11 (± 0.01) mg/kg with percentage recoveries of 93% and 90% for two fertilizers respectively. The Pb content exceeded the maximum permissible limit of 1.0 mg/kg set by the Sri Lanka Standard (SLS 1702:2121), while Cd levels remained within the limit of 0.5 mg/kg. Polymerase chain reaction (PCR) coupled with gel electrophoresis confirmed the presence of the pathogenic O157:H7 E. coli strain in both fertilizers. Thus, the results of this study confirmed the lead (Pb) and Escherichia coli contamination in liquid organic fertilizers in the local market of Sri Lanka.

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