ESCA investigation of low-temperature ammonia plasma-treated polyethylene substrate for immobilization of protein

Hayat, Umar; Tinsley, Anne M.; Calder, Michael R.; Clarke, David J.

doi:10.1016/0142-9612(92)90022-g

Cited by 46 publications

(23 citation statements)

References 22 publications

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“…39,40 It has been found that the substrates with the amine and amide groups promote attachment of cells in the culture. 26,34,[41][42][43][44][45][46] This may result from the selective adsorption and immobilization of proteins on the scaffold surface 27,42,47 and/or the direct interaction of cells with the amine or amide groups. 43 Polyhydroxyacids such as polyglycolide, polylactides, and their copolymers have extensively been investigated as candidate materials for tissue engineered scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Gugala

Gogolewski

2005

J Biomedical Materials Res

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Nonporous and porous membranes from poly(L/DL-lactide) 80/20% were treated with low-temperature oxygen, ammonia, or sulphur dioxide-hydrogen plasmas and the late effects of plasma treatment on physicochemical characteristics of the membranes' surface were analyzed. The plasma treatment resulted in the permanent attachment of sulphur and nitrogen functionalities to the membrane's surface, and increased the surface concentration of oxygen, thereby increasing the surface wettability. To assess whether the plasma treatment affects the cellular response, primary rat osteoblasts were cultured on nontreated and plasma-treated nonporous and microporous membranes, and attachment, growth, and activity of cells were investigated. It was found that attachment and growth of osteoblasts on all the plasma-treated membranes were greater compared with nontreated controls. The treatment with ammonia plasma was most efficacious. The beneficial effects of plasma treatment on cells were most pronounced for microporous polylactide membranes irrespective of the plasma used. The results of the study suggest that the treatment of porous polylactide structures with plasma can be an effective means of enhancing their suitability for tissue engineering. Plasma exposure may also have an advantageous effect on bone healing when polylactide membranes are used to treat bone defects.

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Section: Discussionmentioning

confidence: 99%

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Gugala

Gogolewski

2005

J Biomedical Materials Res

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“…Often, the primary amino (-NH 2 ) groups serve as binding sites for spacer molecules 3 Present address: Novellus Systems, Inc., 3011 N. 1st St, San Jose, CA 95134, USA. 4 Author to whom any correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…Traditionally, low pressure plasmas sustained in N 2 or N-containing gases such as NH 3 are used for such functionalization. Hayat et al [4] used ammonia radio frequency (rf) discharges at 75 mTorr and 10 W to modify polyethylene (PE) surfaces with -NH 2 groups to subsequently immobilize proteins. They found that surface modification occurs rapidly within 1 min of treatment, increasing -NH 2 coverage from 0% up to 7%.…”

Section: Introductionmentioning

confidence: 99%

Repetitively pulsed atmospheric pressure discharge treatment of rough polymer surfaces: II. Treatment of micro-beads in He/NH₃/H₂O and He/O₂/H₂O mixtures

Bhoj

Kushner

2008

Plasma Sources Sci. Technol.

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Plasmas are increasingly being used to functionalize the surface of polymers having complex shapes for biomedical applications such as tissue scaffolds and drug delivering micro-beads. The functionalization often requires affixation of amine (NH 2 ) or O-containing groups. In this paper, results are discussed from a two-dimensional computational investigation of the atmospheric pressure plasma functionalization of non-planar and porous surfaces of polypropylene with NH x and O-containing groups. For the former, the discharge is sustained in He/NH 3 /H 2 O mixtures in a dielectric barrier-corona configuration. Significant microscopic non-uniformities arise due to competing pathways for reactive gas phase radicals such as OH and NH 2 , and on the surface by the availability of OH to initiate amine attachment. The treatment of inside surfaces of porous polymer micro-beads placed on an electrode is particularly sensitive to view angles to the discharge and pore size, and is ultimately controlled by the relative rates of radical transport and surface reactions deep into the pores. The functionalization of micro-beads suspended in He/O 2 /H 2 O discharges is rapid with comparable treatment of the outer and interior surfaces, but varies with the location of the micro-bead in the discharge volume.

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“…The binding energy (E B ) scale was fixed by the assignment of E B ϭ 285.0 eV to the OCH 2 O carbon (1s) peak. On the basis of this reference peak (called C1 in the figures) and according to previous studies, [41][42][43] the carbon chemical shifts for different oxygen-containing groups were C2 (ϾCOOO from hydroxyl, hydroperoxide, ether, or alkyl ester, ⌬E B ϭ 1.5 eV), C3 (ϾCAO from carbonyl or amide, ⌬E B ϭ 3.0 eV), and C4 (OCOOO from carboxyl or the corresponding ester, ⌬E B ϭ 4.2 eV). The areas of the different peaks were computed graphically from the spectra and were corrected with Scofields relative cross sections: 44 /C(1s)/ ϭ 1.00, /O(1s)/ ϭ 2.93, and /N(1s)/ ϭ 1.68.…”

Section: Escamentioning

confidence: 99%

Surface modification of silicone rubber with poly(ethylene glycol) hydrogel coatings

Vladkova

2004

J of Applied Polymer Sci

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Hydrogel coatings of monoacrylated poly-(ethylene glycol) (PEG) methyl ethers of different molecular weights were attached to silicon rubber surfaces and crosslinked with hexanediol diacrylate or ethoxylated trimethylolpropane diacrylate by UV polymerization. The wetting, evaluated with the water contact angles, correlated with the surface oxyethylene chain density, which was evaluated with the ESCA ϾCOOO/OCH 2 O ratio obtained from the C(1s) peak. As measured by the ESCA N(1s) peak, bovine serum albumin formed very thin protein adsorbates on the PEG-coated surfaces. A strong correlation was found between low protein adsorption and a high ϾCOOO/ OCH 2 O ratio of the PEG-coated substrate. The PEG-coated silicon rubber also demonstrated very low cell and platelet adhesion.

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ESCA investigation of low-temperature ammonia plasma-treated polyethylene substrate for immobilization of protein

Cited by 46 publications

References 22 publications

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Repetitively pulsed atmospheric pressure discharge treatment of rough polymer surfaces: II. Treatment of micro-beads in He/NH₃/H₂O and He/O₂/H₂O mixtures

Surface modification of silicone rubber with poly(ethylene glycol) hydrogel coatings

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ESCA investigation of low-temperature ammonia plasma-treated polyethylene substrate for immobilization of protein

Cited by 46 publications

References 22 publications

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Repetitively pulsed atmospheric pressure discharge treatment of rough polymer surfaces: II. Treatment of micro-beads in He/NH3/H2O and He/O2/H2O mixtures

Surface modification of silicone rubber with poly(ethylene glycol) hydrogel coatings

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Repetitively pulsed atmospheric pressure discharge treatment of rough polymer surfaces: II. Treatment of micro-beads in He/NH₃/H₂O and He/O₂/H₂O mixtures