Surface modification of silicone rubber with poly(ethylene glycol) hydrogel coatings

Abstract

Hydrogel coatings of monoacrylated poly-(ethylene glycol) (PEG) methyl ethers of different molecular weights were attached to silicon rubber surfaces and crosslinked with hexanediol diacrylate or ethoxylated trimethylolpropane diacrylate by UV polymerization. The wetting, evaluated with the water contact angles, correlated with the surface oxyethylene chain density, which was evaluated with the ESCA ϾCOOO/OCH 2 O ratio obtained from the C(1s) peak. As measured by the ESCA N(1s) peak, bovine serum albumin formed very thin protein adsorbates on the PEG-coated surfaces. A strong correlation was found between low protein adsorption and a high ϾCOOO/ OCH 2 O ratio of the PEG-coated substrate. The PEG-coated silicon rubber also demonstrated very low cell and platelet adhesion.

Nanotechnology‐Enabled Closed Loop Insulin Delivery Device: In Vitro and In Vivo Evaluation of Glucose‐Regulated Insulin Release for Diabetes Control

“…To overcome this problem, the external surfaces of the device were masked by covalent coupling of a poly(ethyleneglycol) derivative (PEGylation) in an effort to minimize the immune cell response 17. PEGylated silicon rubber has exhibited very low cell and platelet adhesion in vitro in comparison to blank silicone 23…”

Mitigation of Reactive Human Cell Adhesion on Poly(dimethylsiloxane) by Immobilized Trypsin

“…In addition, due to the large amounts of serum present in cerebrospinal fluid (CSF),7 the cells and other living organisms such as bacteria that enter the brain during the insertion of catheter can colonize, proliferate and cause occlusion and/or infections 42. On the other hand, several studies have also reported protein/cell adhesion resistant properties by coating hydrophilic materials such as polyethylene glycol (PEG) on biomaterials 51, 52, 53. However, some reports have highlighted diminished PEG activity in the presence of protein aggregates49(which is the case in vivo) or during long-term use50 making PEGylation not ideal for the current application.…”

“…In addition, because of the large amounts of serum present in cerebrospinal fluid (CSF), the cells and other living organisms such as bacteria that enter the brain during the insertion of the catheter can colonize, proliferate, and cause occlusion and/or infections . However, several studies have also reported protein/cell adhesion-resistant properties by coating hydrophilic materials such as polyethylene glycol (PEG) on biomaterials. − However, some reports have highlighted diminished PEG activity in the presence of protein aggregates (which is the case in vivo) or during long-term use, making PEGylation not ideal for the current application. Similarly, few studies report protein-resistant agents called kosmotropes that create a barrier to proteins when present in solution. , However, to our knowledge, there are no reports showing tethered kosmotropic agents to biomaterials as a means to mitigate whole mammalian cell adhesion.…”

Surface Engineered Polymeric Biomaterials with Improved Biocontact Properties

“…The two-step procedure enhances the EO content at the interface of about twice. Strong hydrophilic (water contact angle <10 • ) protein repellent surfaces (protein adsorption below 0.05 mg/m 2 ) could be prepared in this way on different polymers: PE, polyvinyl chloride, polystyrene, natural rubber, and polydimethyl siloxane [47][48][49][50][51][52]. R. Bischoff and G. Bischoff represent PEG hydrogel covering of polysiloxane tubing and tracheal prostheses preceded by plasma treatment [53].…”