ES, iPS, MSC, and AFS cells. Stem cells exploitation for Pediatric Surgery: current research and perspective

Pozzobon, Michela; Ghionzoli, Marco; Coppi, Paolo De

doi:10.1007/s00383-009-2478-8

Cited by 62 publications

(56 citation statements)

References 85 publications

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“…tissue engineering approaches to treat paediatric congenital cardiovascular malformations), [78] but their use for cell therapy in an allogeneic context (i.e. I/R injury) need further evaluation [79].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Cardiomyogenic Differentiation of Amniotic Fluid Stem Cells

Bollini

Pozzobon

Nobles

et al. 2010

Stem Cell Rev and Rep

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Cell therapy has developed as a complementary treatment for myocardial regeneration. While both autologous and allogeneic uses have been advocated, the ideal candidate has not been identified yet. Amniotic fluid-derived stem (AFS) cells are potentially a promising resource for cell therapy and tissue engineering of myocardial injuries. However, no information is available regarding their use in an allogeneic context. c-kit-sorted, GFP-positive rat AFS (GFP-rAFS) cells and neonatal rat cardiomyocytes (rCMs) were characterized by cytocentrifugation and flow cytometry for the expression of mesenchymal, embryonic and cell lineage-specific antigens. The activation of the myocardial gene program in GFP-rAFS cells was induced by co-culture with rCMs. The stem cell differentiation was evaluated using immunofluorescence, RT-PCR and single cell electrophysiology. The in vivo potential of Endorem-labeled GFP-rAFS cells for myocardial repair was studied by transplantation in the heart of animals with ischemia/reperfusion injury (I/R), monitored by magnetic resonance imaging (MRI). Three weeks after injection a small number of GFP-rAFS cells acquired an endothelial or smooth muscle phenotype and to a lesser extent CMs. Despite the low GFP-rAFS cells count in the heart, there was still an improvement of ejection fraction as measured by MRI. rAFS cells have the in vitro propensity to acquire a cardiomyogenic phenotype and to preserve cardiac function, even if their potential may be limited by poor survival in an allogeneic setting.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Cardiomyogenic Differentiation of Amniotic Fluid Stem Cells

Bollini

Pozzobon

Nobles

et al. 2010

Stem Cell Rev and Rep

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“…Furthermore, although we have demonstrated this finding in the context of a bone-promoting niche, other tissue regeneration paradigms would benefit from a similar characterization of nichestem cell interactions. Finally, from a therapeutic standpoint, the potential exists to fabricate alternative biomimetic scaffolds capable of directing differentiation of h-iPSCs or h-ESCs not just to bone, but also to cartilage, fat, nerve, muscle, glandular tissue, and so forth, yet simultaneously avoid teratoma formation (36,37). Promising results have already been obtained using various nanomaterial scaffolds to craft specific niches supportive of both short-term cellular adhesion and proliferation, as well as longerterm viability, lineage differentiation, and functionalization (38,39).…”

Section: Discussionmentioning

confidence: 99%

In vivo directed differentiation of pluripotent stem cells for skeletal regeneration

Lévi¹,

Hyun²,

Montoro³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

118

117

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Pluripotent cells represent a powerful tool for tissue regeneration, but their clinical utility is limited by their propensity to form teratomas. Little is known about their interaction with the surrounding niche following implantation and how this may be applied to promote survival and functional engraftment. In this study, we evaluated the ability of an osteogenic microniche consisting of a hydroxyapatite-coated, bone morphogenetic protein-2–releasing poly- l -lactic acid scaffold placed within the context of a macroenvironmental skeletal defect to guide in vivo differentiation of both embryonic and induced pluripotent stem cells. In this setting, we found de novo bone formation and participation by implanted cells in skeletal regeneration without the formation of a teratoma. This finding suggests that local cues from both the implanted scaffold/cell micro- and surrounding macroniche may act in concert to promote cellular survival and the in vivo acquisition of a terminal cell fate, thereby allowing for functional engraftment of pluripotent cells into regenerating tissue.

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“…They possess an intermediate differentiation potential between embryonic (pluripotent) and adult cells with advantages over both. Compared to adult cells, AFMSCs possess greater differentiation potential and more primitive properties with fewer accumulated mutations [45] . Respect to embryonic stem cells (ESCs), AFMSCs possess the advantage to do not form teratomas when transplanted in vivo.…”

Section: Amniotic Fluid-derived Cellsmentioning

confidence: 99%

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Pipino

Pandolfi

2015

WJSC

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ES, iPS, MSC, and AFS cells. Stem cells exploitation for Pediatric Surgery: current research and perspective

Cited by 62 publications

References 85 publications

In Vitro and In Vivo Cardiomyogenic Differentiation of Amniotic Fluid Stem Cells

In Vitro and In Vivo Cardiomyogenic Differentiation of Amniotic Fluid Stem Cells

In vivo directed differentiation of pluripotent stem cells for skeletal regeneration

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

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