Erythropoietin Modulates Calcium Influx through TRPC2

Chu, Xin; Cheung, Joseph Y.; Barber, Dwayne L.; Birnbaumer, Lutz; Rothblum, Lawrence I.; Conrad, Kathleen; Abrasonis, Virginia; Chan, Yiu Mo; Stahl, Richard C.; Carey, David J.; Miller, Barbara A.

doi:10.1074/jbc.m205541200

Cited by 54 publications

(66 citation statements)

References 42 publications

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“…The presence of TRPC2 clone 14 (132 kDa) was also confirmed as previously (Ref. 21 and results not shown). Neither anti-TRPM2-N or anti-TRPM2-C antibodies recognized mTRPC2 or hTRPC6.…”

Section: Cloning Of Trpm2-s-trpm2supporting

confidence: 72%

“…One of these antibodies (anti-TRPM2-N) was generated to an epitope in the N terminus of TRPM2 (ILKELSKEEEDTDSSEEMLA, amino acids 658 -677) and recognized both TRPM2-L and TRPM2-S. A second antibody (anti-TRPM2-C) was generated to an epitope in the C terminus of TRPM2 (KAAEEPDAEPG-GRKKTEEPGDS, amino acids 1216 -1237) and was specific for TRPM2-L. Specificity of the antibodies was confirmed using in vitro translation products prepared with cDNAs for mTRPC2 clone 14 (19), hTRPC6 (20), TRPM2-S, and TRPM2-L, cloned into pcDNA3 or pcDNA3.1/V5-His TOPO vector as described previously (21). The in vitro translation products were prepared with the TNT quick coupled transcription/translation system (Promega, Madison, WI).…”

Section: Methodsmentioning

confidence: 99%

“…The pQBI50 vector contains a CMV promoter that drives expression of SuperGlo BFP fused through a flexible linker to TRPM2-L. Successful transfection of 293T cells with TRPM2-S and TRPM2-L was verified by detection of GFP (excitation, 478 nm; emission, 535 nm) and BFP (excitation, 380 nm; emission 435 nm), respectively, in cells with our digital video imaging system (21,22). The fluorescence microscopy-coupled digital video imaging used to measure changes in [Ca 2ϩ ] i has been described previously (22)(23)(24)(25).…”

Section: Methodsmentioning

confidence: 99%

“…ECL was used for detection of signal. The crude membranes were also prepared from 293T cell pellets transfected with vector alone (pcDNA3.1), TRPM2-S, and/or TRPM2-L as described previously (21), and Western blotting was performed as described above.…”

Section: Methodsmentioning

confidence: 99%

“…To confirm that TRPM2-S and TRPM2-L have a similar subcellular distribution and that TRPM2-S does not alter the subcellular localization of TRPM2-L, crude membrane fractions were prepared from 293T cells transfected with vector alone (pcDNA3.1), TRPM2-S, TRPM2-L, or both as described previously (21). Western blotting was performed with protein isolated in the crude membrane pellet or the supernatant, and the blots were probed with anti-TRPM2-N (Fig.…”

Section: Cloning Of Trpm2-s-trpm2mentioning

confidence: 99%

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A Novel TRPM2 Isoform Inhibits Calcium Influx and Susceptibility to Cell Death

Zhang

Chu

Tong

et al. 2003

Journal of Biological Chemistry

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213

248

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TRPM2 is a Ca 2؉ -permeable channel that is activated by oxidative stress and confers susceptibility to cell death. Here, an isoform of TRPM2 was identified in normal human bone marrow that consists of the TRPM2 N terminus and the first two predicted transmembrane domains. Because of alternative splicing, a stop codon (TAG) is located at the splice junction between exons 16 and 17, resulting in deletion of the four C-terminal transmembrane domains, the putative calcium-permeable pore region, and the entire C terminus. This splice variant was found in other hematopoietic cells including human burst forming unit-erythroid-derived erythroblasts and TF-1 erythroleukemia cells. Endogenous expression of both the short form of TRPM2 (TRPM2-S) and the full length (TRPM2-L) was determined by reverse transcriptase-PCR, and localization of endogenous TRPM2 to the plasma membrane was demonstrated by confocal microscopy.

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“…The presence of TRPC2 clone 14 (132 kDa) was also confirmed as previously (Ref. 21 and results not shown). Neither anti-TRPM2-N or anti-TRPM2-C antibodies recognized mTRPC2 or hTRPC6.…”

Section: Cloning Of Trpm2-s-trpm2supporting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cloning Of Trpm2-s-trpm2mentioning

confidence: 99%

See 3 more Smart Citations

A Novel TRPM2 Isoform Inhibits Calcium Influx and Susceptibility to Cell Death

Zhang

Chu

Tong

et al. 2003

Journal of Biological Chemistry

Self Cite

213

248

View full text Add to dashboard Cite

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TRPs in Olfaction

Zufall

2014

Handbook of Experimental Pharmacology

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The mammalian olfactory system has become an excellent model system to understand the function of transient receptor potential (TRP) channels within their native cellular and circuit environment. The discovery that the canonical TRP channel TRPC2 is highly expressed in sensory neurons of the vomeronasal organ (VNO) has led to major advances in our understanding of the cellular and molecular processes underlying signal transduction of pheromones and other molecular cues that play an essential role in the control of instinctive decisions and innate social behaviors. TRPC2 knockout mice provide a striking example that the loss of function of a single gene can cause severe alterations in a variety of social interactions including the display of aggression, social dominance, and sexual behaviors. There is mounting evidence that TRPC2 is not the only TRP channel expressed in cells of the olfactory system but that other TRP channel subtypes such as TRPC1, TRPC4, TRPC6, TRPM4, and TRPM5 could also play important functional roles in mammalian olfaction. Here, I review such findings and discuss future areas for investigation.

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