Erythrocytosis, glomerulomegaly, mesangial hyperplasia, sialyl hyperplasia, and arterioscleriosis induced in rats by nickel subsulfide

Ks, McCully; La, Rinehimer; Cg, Gillies; Hopfer, Sidney M.; Fw, Sunderman

doi:10.1007/bf00430666

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…The cell type they described in their cultures grew stacking and overlapping but the exact nature of these cells was not confirmed. Recently, McCully et al (35) reported mesangial hyperplasia and erythrocytosis in rats after intrarenal injection of nickel subsulfide. They concluded that mesangial cells are implicated in Ep production.…”

Section: Discussionmentioning

confidence: 99%

Renal mesangial cell cultures as a model for study of erythropoietin production.

Kurtz¹,

Jelkmann²,

Sinowatz³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Mesangial cells derived from isolated glomeruli of rat kidney were grown as homogeneous cell lines in culture. They released, into the culture medium, erythropoietin that had free terminal galactosyl residues and was therefore not active in vivo. The production of erythropoietin by these cells was significantly enhanced by either lowering the Po2 in the incubation atmosphere or by adding cobalt chloride to the culture medium. Therefore, mesangial cells in culture may be considered as an in vitro system in which the regulation of erythropoietin production can be studied under well-defined conditions. With this in mind, we sought to develop a cell culture system in which the biosynthesis of Ep can be studied under conditions that are much better defined than in the intact organ. We show here that renal glomerular cells, which were clearly identified to be of mesangial origin, in culture produce Ep that has free galactosyl residues. The Ep production of these cells could be enhanced by hypoxia and cobalt chloride which are well-known stimuli of Ep formation in vivo. Therefore, the present culture system can be regarded as a useful model for the study of the hypoxia-induced increase of Ep synthesis. MATERIALS AND METHODSThe preparation of isolated glomeruli from rat kidneys and subculture of glomerular outgrowths were done as described (5).Cells subcultured 3 weeks after the first inoculation were used. The cells were grown in RPMI 1640 (Boehringer Mannheim) supplemented with 10% fetal bovine serum (Boehringer Mannheim), penicillin at 100 units/ml, streptomycin at 10 jig/ml, and bovine insulin (Sigma) at 0.66 unit/ml. Kalden et al. (11).Ep Assay. The Ep activity of the culture medium was determined with the fetal mouse liver cell test (12) and a serumfree incubation mixture (6). In some experiments, bone marrow cells from adult mice were used for the test. The total volume of one test was 600 ,ul including 60 ,Al of the sample to be tested for Ep activity. Standard Ep (human urinary Ep standardized against International Reference Preparation B) was kindly provided by the National Institutes of Health. All unknown samples from the culture medium were chromatographed on DEAE-cellulose at pH 4.5 (13)

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Section: Discussionmentioning

confidence: 99%

Renal mesangial cell cultures as a model for study of erythropoietin production.

Kurtz¹,

Jelkmann²,

Sinowatz³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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“…This reaction, due to induction of erythropoietin, involves erythroid hyperplasia of bone marrow and spleen [111][112][113][114]. Guinea pigs, but not hamsters and gerbils, respond to intrarenal nickel subsulfi de in the same way [115].…”

Section: Kidneysmentioning

confidence: 99%

“…Guinea pigs, but not hamsters and gerbils, respond to intrarenal nickel subsulfi de in the same way [115]. Morphological effects of Ni 3 S 2 in the kidney include glomerulomegaly, tubular hyperplasia, and arteriosclerosis [112].…”

Section: Kidneysmentioning

confidence: 99%

Nickel Toxicity and Carcinogenesis

Kasprzak

Salnikow

2007

Nickel and Its Surprising Impact in Nature

123

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“…In animals that were given a-Ni3Sz (Morse et ul. 1977;Hopfer et al 1978Hopfer et al & 1979McCully et al 1982) findings similar to that found by mesangial cell hyperplasia and glonierulomegaly in human subjects with hypoxia and erythrocytosis, were made. a-Ni3Sz a shift of oxidative metabolism in renal cells might mimic renal cell hypoxia, which has been shown to stimulate erythropoietin production, and hypcrplasia of bone marrow.…”

Section: Discussionmentioning

confidence: 60%

The Effect of Intrarenal Nickel Subsulfide Injections upon the Activity of Selected Erythrocyte and Bone Marrow Enzymes in Rats

Miszta

Dabrowski

Szyguła

et al. 1986

Acta Pharmacologica et Toxicologica

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The experiments were carried out on Wistar rats. The animals were divided into five groups: Group I: control group; Group 2: the animals received a single intrarenal dose of nickel subsulfide (Ni3S2) in saline; Group 3: the animals receiving a singlc intrarcnal dose of saline; Group 4: the animals receiving a single intrarenal dose of Ag2S in saline; Group 5: the animals receiving a single intrarenal dose of activated charcoal in saline. Prior to the procedure peripheral blood indices were determined. The experiments included animals manifesting polycythemia. Samples of blood and bone marrow were collected and the activities of AchE, GR and G6PD were determined. An increase in the activity of the three studied enzymes was detected in Groups no. 2 and 5.

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Erythrocytosis, glomerulomegaly, mesangial hyperplasia, sialyl hyperplasia, and arterioscleriosis induced in rats by nickel subsulfide

Cited by 9 publications

References 23 publications

Renal mesangial cell cultures as a model for study of erythropoietin production.

Renal mesangial cell cultures as a model for study of erythropoietin production.

Nickel Toxicity and Carcinogenesis

The Effect of Intrarenal Nickel Subsulfide Injections upon the Activity of Selected Erythrocyte and Bone Marrow Enzymes in Rats

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