Erythrocytes Reduce Extracellular Ascorbate Free Radicals Using Intracellular Ascorbate as an Electron Donor

VanDuijn, Martijn M.; Tijssen, Karmi; VanSteveninck, John; Broek, Peter J.A. Van den; Zee, Jolanda Van der

doi:10.1074/jbc.m910281199

Cited by 64 publications

(50 citation statements)

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“…The GSH concentration was routinely tested with DTNB as indicated above for cysteine quantification, and the concentration of ascorbate was monitored with measurements of the absorbance at 265 nm, using an extinction coefficient of 14 500 M -1 ‚cm -1 (46).…”

Section: Measurement Of Peroxynitrite Produced During Sin-1 Decomposimentioning

confidence: 99%

Inhibition of Skeletal Muscle S1-Myosin ATPase by Peroxynitrite

et al. 2006

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Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg 2+ -ATPase activity, reaching 50% inhibition with 46.7 ( 8.3 µM SIN-1 for 8.7 µM S1, that is, at a SIN-1/S1 molar ratio of approximately 5.5. The inhibition was due to the peroxynitrite produced by SIN-1 decomposition because (1) decomposed SIN-1 was found to have no effect on S1 ATPase activity, (2) addition of SIN-1 in the presence of superoxide dismutase and catalase fully prevented inhibition by SIN-1, and (3) micromolar pulses of chemically synthesized peroxynitrite produced inhibition of F-actin-stimulated S1 Mg 2+ -ATPase activity. In parallel, SIN-1 produced the inhibition of the nonphysiological Ca 2+ -dependent and K + /EDTA-dependent S1 ATPase activity of S1 and, therefore, suggested that the inhibition of F-actin-stimulated S1 Mg 2+ -ATPase activity is produced by the oxidation of highly reactive cysteines of S1 (Cys 707 and Cys 697 ), located close to the catalytic center. This point was further confirmed by the titration of S1 cysteines with 5,5′-dithiobis(2-nitrobenzoic acid) and by the parallel decrease of Cys 707 labeling by 5-(iodoacetamido)fluorescein, and it was reinforced by the fact that other common protein modifications produced by peroxynitrite, for example, protein carbonyl and nitrotyrosine formation, were barely detected at the concentrations of SIN-1 that produced more than 50% inhibition of the F-actin-stimulated S1 Mg 2+ -ATPase activity. Differential scanning calorimetry of S1 (untreated and treated with different SIN-1 concentrations) pointed out that SIN-1, at concentrations that generate micromolar peroxynitrite fluxes, impaired the ability of ADP‚V 1 to induce the intermediate catalytic transition state and also produced the partial unfolding of S1 that leads to an enhanced susceptibility of S1 to trypsin digestion, which can be fully protected by 2 mM GSH.

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Section: Measurement Of Peroxynitrite Produced During Sin-1 Decomposimentioning

confidence: 99%

Inhibition of Skeletal Muscle S1-Myosin ATPase by Peroxynitrite

et al. 2006

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“…Il permet par ailleurs une polymérisation complète des radicaux libres de l'adhésif et la formation d'une couche hybride étanche et uniforme à l'interface résine/dent [34,35,36]. L'ascorbate de sodium est préféré à l'acide ascorbique pour éviter l'effet de double mordançage à l'A.…”

Section: Les Antioxydants Non Enzymatiquesunclassified

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Aguir

Jemâa

Jalouli

et al. 2018

Actual. Odonto-Stomatol.

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“…Unlike its effect on AA: ferricyanide oxidoreductase activity, pCMBS does not affect the preservation of extracellular AA. Van Duijn et al (28) found that low NADH concentrations resulted in higher rates of AA oxidation to AFR, while the opposite was found for high NADH concentrations, indicating that NADH is a necessary requirement for AA regeneration from AFR. This group speculated on the functional components of the electron transport system claiming that an a-TH shuttle was an unlikely candidate given the redox potential ( Table 2) and electrogenic nature of the transport.…”

Section: Redox Half Reaction E8 (Mv)mentioning

confidence: 99%

“…The more hyperpolarized the membrane, the faster the AFR reduction (17). Enhancement of AFR reduction can also be achieved by increasing the intracellular AA concentration (28). The reductase activity is inhibited by NEM, which brings about a loss of available GSH, presumably the basis of decreased intracellular AA recycling (27).…”

Section: Redox Half Reaction E8 (Mv)mentioning

confidence: 99%

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Redox Reactions and Electron Transfer Across the Red Cell Membrane

Kennett

Kuchel

2003

IUBMB Life

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SummaryPlasma membrane electron transport systems appear to be ubiquitous. These systems are implicated in hormone signal transduction, cell growth and differentiation events as well as protection from oxidative stress. The red blood cell is constantly exposed to oxidative stress; protection against the reactive species generated during this process may be the main role of its membrane electron transport systems. Membrane redox activity has been studied for over three-quarters of a century, and yet many questions remain regarding its identity and likely roles: are electron transfers by distinct and specific mechanisms; what are the physiological donors and acceptors; and how do these systems affect metabolism? Current evidence suggests that the human erythrocyte membrane contains a number of distinct electron transfer systems, some of which, at least, involve membrane proteins, and NADH or ascorbate as electron donors. The activity of these systems appears to be closely related to the metabolic state of the cell, suggesting that mediation of reducing equivalents across the plasma membrane allows redox buffering of each environment, intra-and extracellular, by the other. We have decided to study this from a new perspective, NMR spectroscopy the area of our own technical expertise, hence this review is slanted towards this more recent analysis. IUBMB Life, 55: 375-385, 2003

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Erythrocytes Reduce Extracellular Ascorbate Free Radicals Using Intracellular Ascorbate as an Electron Donor

Cited by 64 publications

References 45 publications

Inhibition of Skeletal Muscle S1-Myosin ATPase by Peroxynitrite

Inhibition of Skeletal Muscle S1-Myosin ATPase by Peroxynitrite

Particularités du collage aux dents qui ont subi un éclaircissement

Redox Reactions and Electron Transfer Across the Red Cell Membrane

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