Redox Reactions and Electron Transfer Across the Red Cell Membrane

Kennett, Eleanor C.; Kuchel, Philip W.

doi:10.1080/15216540310001592843

Cited by 44 publications

(26 citation statements)

References 40 publications

(115 reference statements)

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“…Glucose is the main energy source of RBCs, supporting glycolysis and pentose phosphate pathway (PPP) reduction of NADP ϩ to NADPH, a cofactor of GSH reductase (GR), which maintains the intracellular GSH pool. GSH may also participate in transmembrane electron transfer to reduce exofacial thiols (16). H 2S production then leads to vasorelaxation via vascular smooth muscle cell K ATP-linked hyperpolarization (8).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide mediates the vasoactivity of garlic

Benavides

Squadrito

Mills

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

756

647

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The consumption of garlic is inversely correlated with the progression of cardiovascular disease, although the responsible mechanisms remain unclear. Here we show that human RBCs convert garlic-derived organic polysulfides into hydrogen sulfide (H2S), an endogenous cardioprotective vascular cell signaling molecule. This H2S production, measured in real time by a novel polarographic H2S sensor, is supported by glucosemaintained cytosolic glutathione levels and is to a large extent reliant on reduced thiols in or on the RBC membrane. H2S production from organic polysulfides is facilitated by allyl substituents and by increasing numbers of tethering sulfur atoms. Allyl-substituted polysulfides undergo nucleophilic substitution at the ␣ carbon of the allyl substituent, thereby forming a hydropolysulfide (RSnH), a key intermediate during the formation of H2S. Organic polysulfides (R-Sn-R; n > 2) also undergo nucleophilic substitution at a sulfur atom, yielding RSnH and H2S. Intact aorta rings, under physiologically relevant oxygen levels, also metabolize garlic-derived organic polysulfides to liberate H2S. The vasoactivity of garlic compounds is synchronous with H2S production, and their potency to mediate relaxation increases with H2S yield, strongly supporting our hypothesis that H2S mediates the vasoactivity of garlic. Our results also suggest that the capacity to produce H2S can be used to standardize garlic dietary supplements.Allium ͉ aorta ͉ polysulfides ͉ red blood cells ͉ vasorelaxation

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Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide mediates the vasoactivity of garlic

Benavides

Squadrito

Mills

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

756

647

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“…[1][2][3] There are a lot of studies on the enzyme-catalyzed electron transport in biomembranes. [4][5][6] The details of the electron transports in biomembranes, however, have not been well elucidated owing mainly to the complexity of the living organisms. On the other hand, some voltammetric studies have been carried out to examine the electron transport due to membrane-bound redox enzymes on solid electrodes modified with self-assembled monolayers (SAMs) or supported bilayer lipid membranes (sBLMs).…”

Section: Introductionmentioning

confidence: 99%

Coupling of Proton Transport across Planar Lipid Bilayer and Electron Transport Catalyzed by Membrane-bound Enzyme <small>D</small>-Fructose Dehydrogenase

et al. 2016

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Proton transport from an aqueous phase (W1) to another one (W2) across a planar bilayer lipid membrane (BLM) was driven by the electron transport system. The electron transport system was composed of the oxidation of D-fructose by an oxidized form of D-fructose dehydrogenase (FDH) at the W1|BLM interface, the oxidation of a reduced form of FDH by an oxidized form of 7,7,8,8-tetracyanoquinodimethane (TCNQ) at the W1|BLM interface and the oxidation of a reduced form of TCNQ by [Fe(CN) 6 ] 3− at the W2|BLM interface. Then the negative current due to the electron transfer from W1 to W2 was clearly observed around 0 V. The zero-current potential varied to hold the electroneutrality in all phases by balancing the proton transport with the electron transport.

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“…In particular, a close link between t-PMET and metabolic status of erythrocytes has been reported (141,142).…”

Section: Blood Redox Homeostasismentioning

confidence: 97%

Trans-Plasma Membrane Electron Transport in Mammals: Functional Significance in Health and Disease

Principe

Avigliano

Savini

et al. 2011

Antioxidants & Redox Signaling

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Trans-plasma membrane electron transport (t-PMET) has been established since the 1960s, but it has only been subject to more intensive research in the last decade. The discovery and characterization at the molecular level of its novel components has increased our understanding of how t-PMET regulates distinct cellular functions. This review will give an update on t-PMET, with particular emphasis on how its malfunction relates to some diseases, such as cancer, abnormal cell death, cardiovascular diseases, aging, obesity, neurodegenerative diseases, pulmonary fibrosis, asthma, and genetically linked pathologies. Understanding these relationships may provide novel therapeutic approaches for pathologies associated with unbalanced redox state.

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Redox Reactions and Electron Transfer Across the Red Cell Membrane

Cited by 44 publications

References 40 publications

Hydrogen sulfide mediates the vasoactivity of garlic

Hydrogen sulfide mediates the vasoactivity of garlic

Coupling of Proton Transport across Planar Lipid Bilayer and Electron Transport Catalyzed by Membrane-bound Enzyme <small>D</small>-Fructose Dehydrogenase

Trans-Plasma Membrane Electron Transport in Mammals: Functional Significance in Health and Disease

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