Erythrocytes and cell line-based assays to evaluate the cytoprotective activity of antioxidant components obtained from natural sources

Abstract

Oxidative stress can damage cellular components including DNA, proteins or lipids, and may cause several skin diseases. To protect from this damage and addressing consumer's appeal to natural products, antioxidants obtained from algal and vegetal extracts are being proposed as antioxidants to be incorporated into formulations. Thus, the development of reliable, quick and economic in vitro methods to study the cytoactivity of these products is a meaningful requirement.A combination of erythrocyte and cell line-based assays was performed on two extracts from Sargassum muticum, one from Ulva lactuca, and one from Castanea sativa. Antioxidant properties were assessed in erythrocytes by the TBARS and AAPH assays, and cytotoxicity and antioxidant cytoprotection were assessed in HaCaT and 3T3 cells by the MTT assay. The extracts showed no antioxidant activity on the TBARS assay, whereas their antioxidant capacity in the AAPH assay was demonstrated. On the cytotoxicity assays, extracts showed low toxicity, with IC50 values higher than 200 µg/mL. C. sativa extract showed the most favourable antioxidant properties on the antioxidant cytoprotection assays; while S. muticum and U. lactuca extracts showed a low antioxidant activity. This battery of methods was useful to characterize the biological antioxidant properties of these natural extracts.

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

“…Noteworthy, some extracts previously described in the literature as good antioxidants, namely algae, Ulva lactuca Linnaeus (Ulvaceae) and Sargassum muticum Fensholt (Sargassaceae), and the chestnut Castanea sativa Mill. (Fagaceae) were not able to protect lipids from the membrane against the H 2 O 2 -induced peroxidation, even at the highest tested concentration (Botta et al, 2014).…”

Effect of Baechu Kimchi Added Ecklonia cava Extracts on High Glucose-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

“…Previous studies have reported that high concentrations of glucose can cause endothelial cell damage, leading to increased cell apoptosis in diabetes ( 21 ). The extracts from natural products such as vegetables and seaweeds significantly increase cell viability under conditions of oxidative damage ( 22 , 23 ). In this study, BKE treatment significantly increased endothelial cell viability.…”

Study of pomegranate (Punica granatum L.) peel extract containing anthocyanins on fatty streak formation in the renal arteries in hypercholesterolemic rabbits

“…After that tubes were centrifuged (3000 rpm for 10 min) and absorption of the supernatant solution at 540 nm was measured using a spectrophotometer. [ 26 ]…”