Background
Ursolic acid (UA) is an important bioactive component in many traditional medicinal plants including pomegranate (
Punica granatum
L.) flower.
Methods
This study presents the HPLC analysis of UA contents of ten cultivars of pomegranate flower grown in Iran. The UA contents of fallen flowers of pomegranate were given in each cultivar.
Results
Remarkable quantities of UA were found in ten cultivars of Iranian pomegranate flower evaluated (21.736 to 15.119 mg/g). Lower quantities of UA were determined in pomegranate fallen flowers (16.763 to 5.754 mg/g).
Conclusion
UA values obtained from Iranian cultivars of pomegranate flowers are very significant when compared with other sources of UA. All of the analyzes suggested that the Iranian pomegranate flowers (including flowers on branches and fallen flowers) might be an excellent UA rich source.
Ursolic acid (UA), is a major bioactive compound in several traditional medicinal plants including pomegranate (Punica granatum L.) flower. This study presents the HPLC analysis of UA content in flowers of an Iranian pomegranate cultivar. The analytical method’s accuracy and the repeatability were satisfactory. The Linear ranges were 100-400 µg/ml for UA with a good correlation. On the average, the recovery rate of UA was 100%. Here, the extraction of UA from an Iranian pomegranate flower cultivar by employing an ultrasound-assisted extraction method was reported, and several extraction solvents were studied to improve extraction yield of UA. Remarkable quantities of UA were found in cultivar of pomegranate flower evaluated. Our results showed that Iranian pomegranate flower cv. Ghorj Tafti Torsh is a good source for extraction and isolation of UA.
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