Erythrocyte Sodium Concentration and 86Rb Uptake in Weanling Dahl Rats

McCormick, C P; Hennessy, John F.; Rauch, Albert; Buckalew, Vardaman M.

doi:10.1093/ajh/2.8.604

Cited by 12 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, RBC of SS/Jr rats had higher Na i + content than RBC from SR/Jr rats, which is in accordance with the findings of McCormick et al (1989) and Zicha and Duhm (1990). On the other hand, we did not observe any significant difference between Dahl strains in the activity of the Na + -K + pump, which is in contrast with our previous studies reporting enhanced activity of the Na + -K + pump and Na + -K + cotransport as well as augmented Na + leak and Rb + leak in RBC of Dahl rats with salt hypertension (Zicha and Duhm, 1990;Zicha et al, 2001;Vokurková et al, 2003).…”

Section: Discussionsupporting

confidence: 92%

Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

et al. 2005

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 92%

Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

et al. 2005

View full text Add to dashboard Cite

“…Nevertheless, the observed differences were much smaller than those reported by McCormick et al 13 in fresh red blood cells or by Knorr et al 12 in Na + -loaded erythrocytes of Dahl rats. Moreover, the differences in cell Na + were not augmented by Na + loading procedure used in our experiments (unpublished data from our laboratory).…”

Section: Red Blood Cell Na + Contentcontrasting

confidence: 66%

“…Under the conditions of low salt intake, ouabain-sensitive and furosemide-sensitive Rb + uptakes as well as the Rb + leak tended to be higher in red blood cells of DS/Jr relative to DR/Jr rats, 8 whereas ouabain-sensitive Rb + uptake was similar in DS and DR rats. 13 A high salt diet did not reduce ouabain-sensitive Rb + uptake in either the young or adult DS/Jr group. 8 In contrast, an acceleration of ouabain-sensitive Rb + uptake was found in young DS/Jr rats.…”

Section: With the Technical Assistance Ofjutta Kronauermentioning

confidence: 83%

Kinetics of Na+ and K+ transport in red blood cells of Dahl rats. Effects of age and salt.

Zicha¹,

Duhm²

1990

Hypertension

View full text Add to dashboard Cite

Blood pressure response to chronic high salt intake and kinetics of red blood cell Na + and K + (Rb + ) transport were studied in salt-sensitive (DS) and salt-resistant (DR) Dahl rats fed a high salt diet (8% NaCl) for 7 weeks from the fifth (young), 12th (adult), or 23rd (old) week of age. The kinetics of ouabain-sensitive Rb + uptake and Na + extrusion were determined in Na + media as a function of both intracellular Na + (Na + ,, 2-8 mmol/1 cells) and extracellular Rb + (Rb + 0 ). In addition, the kinetics of furosemide-sensitive Rb + uptake (related to Rb + 0 ) and the magnitude of the Na + and Rb + leaks were assessed. High salt intake induced hypertension in young and adult but not in old DS rats although red blood cell Na + was slightly increased in all age groups of DS rats fed a high salt diet The kinetic parameters of the Na + -K + pump were similar in DS and DR rats fed a low salt diet Ouabain-sensitive transport rates were not suppressed in erythrocytes of salt hypertensive Dahl rats. Maximal velocities of the Na + -K + pump (related to Na + ,) decreased significantly with age in all groups except in DS rats fed a high salt diet This was compensated by an age-dependent increase in the affinity for Na + , so that no substantial differences in transport rates between young and old rats were seen at physiological cell Na + and plasma K + levels. The maximal velocity of furosemide-sensitive Rb + uptake rose with age in all groups without changes in the affinity for Rb + 0 . High salt intake increased its maximal activity only in DS rats. The Rb + leak was higher in DS relative to DR rats irrespective of salt intake, age, or blood pressure. The Na + leak was elevated in DS rats in which it was augmented by high salt intake. Salt hypertension was, however, not associated with a significant increase of the Na + leak, which was responsible for most alterations of cell Na + content (Hypertension 1990;15:612-627) M any previous studies have indicated the role of genetic factors, salt intake, agedependent susceptibility, and ion transport alterations in the development of hypertension. Nevertheless, the interactions of all these factors have seldom been studied under the same experimental conditions. Dahl salt-sensitive (DS) rats with their age-dependent susceptibility to salt hypertension 1 represent an experimental model that appears to be suitable for such an analysis.From the Institute of Physiology, University of Munich, Munich, Germany.J.Z. was the recipient of a research fellowship from the Alexander von Humboldt Foundation.Presented in part at the 66th Meeting of the German Physiological Society in Wurzburg, September 1988, and published in abstract form (Pflugcrs Arch 1988;412(suppl 1):R51).Address for reprints: Prof. J. Duhm, Institute of Physiology, University of Munich, Pettenkoferstr. 12, D-8000 Munchen 2, FRG.Received May 16, 1989; accepted in revised form February 12, 1990. The information on ion transport in vascular smooth muscle and red blood cells of DS and saltresistant rats (DR) is rath...

show abstract

“…30 Briefly, erythrocytes (RBC) from 1 mL of blood were washed three times with ice-chilled isosmotic lithium chloride. After the last wash, 250 fiL of the packed RBC was resuspended in 250 ^L lithium chloride.…”

Section: Measurement Of Erythrocyte Sodium Concentrationmentioning

confidence: 99%

High sensitivity to salt in kininogen-deficient brown Norway Katholiek rats.

et al. 1993

View full text Add to dashboard Cite

Brown Norway Katholiek rats, which have very low levels of plasma kininogens, excreted a much smaller amount of kinin in the urine than normal rats of the same strain. The systolic blood pressure of 7-week-old kininogen-deficient rats fed low (0.3%) NaCI diets (131±4 mm Hg, n=12) was not different from that in normal rats. Two percent NaCI diets given from 7 weeks of age for 4 weeks caused rapid increases in blood pressure (167±4 mm Hg, n=12, 9 weeks old) in deficient rats, although the same diets induced no blood pressure increase in normal rats. Urinary excretion of active kallikrein and prokallikrein remained constant in both rat groups throughout NaCI loading. During this period, the deficient rats secreted less urine (9 weeks old, P<.05) and less urinary sodium (11 weeks old, P<.05). Serum levels of sodium in deficient rats were higher (/ > <.O5) than in normal rats at 9 weeks of age. Intracellular concentrations of sodium in the erythrocytes of deficient rats were higher (P<.05) than in normal rats throughout NaCI loading. Subcutaneous infusion of bovine low molecular weight kininogen with an osmotic pump in NaCI-loaded deficient rats induced a reduction (P<.01) in blood pressure and increases (P<.05) in urine volume and urinary sodium and kinin levels. By contrast, subcutaneous infusion of the bradykinin antagonist Hoe 140 or of aprotinin in NaCI-loaded normal rats induced a hypertensive response. This antagonist treatment reduced urine volume and urinary sodium. These results indicate that the lack of kinin generation observed in the kininogen-deficient rats was related through sodium retention to the hypertensive response to NaCI loading. (Hypertension. 1993;22:705-714.) KEY WORDS • hypertension, sodium-dependent • sodium, dietary • kininogens • bradykinin • aprotininT he blood pressure-lowering effects of urinary kallikrein injected intravenously have been described for more than six decades,' and bradykinin is well known to induce vasodilatation and an increase in renal blood flow and excretion of water and sodium from the kidney.2 Urinary kallikrein therefore has been thought to be involved in hypertension, and its reduction has been reported in human 36 and animal 717 experiments. On the other hand, it is widely accepted that sodium retention may be related to the pathogenesis of hypertension, although the precise mechanisms of its contribution to the elevation of blood pressure are still unclear. The causal relation between these three factors -urinary kallikrein, sodium retention, and hypertension-has not been verified. Recently, we reported that the kallikrein-kinin system may play a suppressive role in deoxycorticosterone acetate (DOCA)-salt hypertension.18 Using kininogendeficient Brown Norway Katholiek (BN-Ka) rats and normal rats of the same strain (Brown Norway Kitasato [BN-Ki]), we were able to demonstrate that the urinary kallikrein-kinin system may contribute to lowering of systemic blood pressure in the initial phase of the development of DOCA-salt hypertension in uninephrectomized ...

show abstract

Erythrocyte Sodium Concentration and 86Rb Uptake in Weanling Dahl Rats

Cited by 12 publications

References 0 publications

Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

Kinetics of Na+ and K+ transport in red blood cells of Dahl rats. Effects of age and salt.

High sensitivity to salt in kininogen-deficient brown Norway Katholiek rats.

Contact Info

Product

Resources

About