Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform

Abstract

Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nanoparticles revealed superior circulation half-life by the erythrocytemimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.biomimetic nanoparticle | drug delivery | long circulation | red blood cell membrane

The impact of nanoparticle protein corona on cytotoxicity, immunotoxicity and target drug delivery

“…Hu et al demonstrated that red blood cell molecules were correctly transferred and oriented on the NPs surfaces [159]. Other bio-inspired NPs whose surface is functionalized through the integration of cell membranes, have been developed [153,[156][157]160].…”

Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

“…[160] On the other hand, Liangfang Zhang research group developed fashion PLGA nanoparticles with lipidic and protein membranes isolated from RBCs as a biomimetic camouflaged delivery system. [161] The elimination half-time for RBC-membrane-coated nanoparticles (RBC-NPs) and PEGcoated nanoparticles was calculated based on two-compartment model after their in vivo administration. Agreeing with the results, the elimination half-life of RBC-NPs (39.6 h) was considerably higher than PEG-coated nanoparticles (15.8 h), suggesting a new method to overcome the low circulation lifetimes of PLGA-based DDSs.…”

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

“…The positive qualities of inorganic and organic carriers can be combined with further processing, e.g. by covering the surfaces of polymer nanocarriers with ghost membranes [15]. This way a camouflaged nanoparticle with the external properties of an erythrocyte can be achieved, whereas the applicability of the polymer core is retained.…”