Erythrocyte Glutathione-levels in Patients of a Mental Hospital

Ellman, George L.; Gan, George L.

doi:10.1038/202904a0

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…Reduced GSH was determined by the method followed by Ellman and Gan. 36 Briefly, 1.0 mL of supernatant was treated with 0.5 mL of Ellman’s reagent and 3.0 mL of phosphate buffer (0.2 M, pH 8.0). The absorbance was read at 412 nm.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats

Ramkumar

Vanitha

et al. 2013

Hum Exp Toxicol

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In the present study, we investigated the antioxidant effect of gallic acid (GA) on membrane lipid peroxidation and osmotic fragility in alloxan-induced diabetic Wistar rats. GA was administered orally at doses of 5, 10, and 20 mg/kg body weight for 45 days, after which liver and kidney tissues were analyzed for the degree of lipid peroxidation, reduced glutathione, and the activities of antioxidants such as catalase, superoxide dismutase, and glutathione peroxidase. Administration of GA to alloxan-induced diabetic rats reduced the blood glucose level with an increase in the level of insulin. Liver and kidney tissues from diabetic animals exhibited disturbances in antioxidant defense compared with normal rats. GA at a dose of 20 mg/kg b.w. showed a significant effect than that of the other doses. In addition, the results revealed that GA protected the integrity of erythrocyte membrane in diabetic rats as demonstrated by lower percentage of hemolysis and resistance to hydrogen peroxide-induced peroxidation. The anti-hyperglycemic activity of GA in alloxan-induced diabetic rats was also comparable with glibenclamide, a reference drug. These results suggest that GA could provide a beneficial effect on diabetes by decreasing oxidative stress-related diabetic complications.

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Section: Methodsmentioning

confidence: 99%

Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats

Ramkumar

Vanitha

et al. 2013

Hum Exp Toxicol

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“…Derivatization of GSH usually increases the method specificity and sensitivity. Recently, several HPLC methods utilizing pre-column derivatization with colorimetric reagents, such as Ellman's [121][122][123][124] or Sanger's [125] reagents, have been described. Ellman's reagent is widely used for the analysis of thiols in biological samples via the determination of the liberated thionitrobenzoate anion [126].…”

Section: Derivatizationmentioning

confidence: 99%

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Iwasaki

Saito

Nakano

et al. 2009

Journal of Chromatography B

100

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“…The placed test samples were centrifuged (1,000×g; 10 min) and observed at 532 nm. 7 Glutathione (GSH) levels were experimented by following the method of Ellman et al, (1964). The Ellman's reagent (3 mL) was added to a serum sample (20 μL) by incubating for 15 min; further, the absorbance level was experimented at 412 nm.…”

Section: Assessment Of Oxidative Stress and Antioxidant Levelsmentioning

confidence: 99%

“…The Ellman's reagent (3 mL) was added to a serum sample (20 μL) by incubating for 15 min; further, the absorbance level was experimented at 412 nm. 8 Catalase (CAT) activity was determined according to Aebi et al (1984). Furthermore, the serum test samples of (1.4 mL) were mixed with 0.1 mL PBS and 1.4 mL 20 mM H 2 O 2 (1:4:5) and studied at 25ºC within 2 min.…”

Section: Assessment Of Oxidative Stress and Antioxidant Levelsmentioning

confidence: 99%

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Sivanandam,

Senthilkumar,

Dipti

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Nandrolone decanoate (ND) is a commonly used androgen-based steroid that causes adverse effects on illicit self-administered users to boost their physical endurance. Although, it has an anabolic effect on the skeletal muscles, plausible evidence supports that the gonadal toxicity of ND can cause damage to reproductive functions. Aim: The present study investigated the possible protective effects of Withania somnifera (WS) root extract (200 and 400 mg/kg bw) on ND-induced (10 mg/kg bw) male gonadal toxicity in rats. Materials and Methods:The present seminal research was not experimented in any of the earlier studies. ND was induced in rats by intramuscular injection bi-weekly for 30 days. The aqueous extract of WS root powder was delivered orally once daily for 30 days to the ND-treated group. Hematoxylin-and-eosin-marked sections of the testes were studied. Results: The testosterone levels significantly decreased while there was an increase in the malondialdehyde levels among the ND-induced group against the control group. The ND induced rats treated with varying doses of WS showed a significant reversal of the gonadal toxicity by improvement in the testosterone and antioxidant parameters. The sperm count and sperm motility rate were increased significantly after WS therapy. Furthermore, the testis morphology of the WS-treated group indicated preserved germinal cells, active spermatogenesis, and absence of atrophy, vacuolation, and necrosis. Conclusion: WS treatment in ND-induced male rats alleviated the toxic effects of the ND on the gonads, probably due to its anti-inflammatory and antioxidant properties.

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Erythrocyte Glutathione-levels in Patients of a Mental Hospital

Cited by 9 publications

References 5 publications

Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats

Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

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