Erythrocyte antioxidants enzymes imbalance following subcutaneous pyrethroid treatments in rats of different sex

Chargui, Issam; Falcioni, Maria Letizia; Cheikh, Hassen Ben; Gabbianelli, Rosita

doi:10.1016/j.etap.2010.04.005

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…Kale et al [5] demonstrated that pesticides through LPO can disturb the biochemical and physiological functions of the red blood cells. The result recorded in the present study is consistent with reports that the OP, CPF [6] , [7] , [25] and pyrethroids, [26] , [27] individually induce an elevation in erythrocyte MDA concentration in exposed rats. The elevated erythrocyte MDA concentration was more pronounced in the co-exposed group compared to either the CPF or DLT alone group.…”

Section: Discussionsupporting

confidence: 93%

Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

et al. 2014

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The present study aimed to evaluate the effect of chronic co-exposure to chlorpyrifos (CPF) and deltamethrin (DLT) on erythrocyte osmotic fragility, lipid peroxidation and the ameliorative effect of alpha-lipoic acid (ALA) on erythrocyte fragility. Thirty-six male Wistar rats divided into six groups of six rats each were used for the study. Groups I (S/oil) and II (ALA) were given soya oil (2 ml/kg) and ALA (60 mg/kg), respectively. Rats in group III (DLT) and IV (CPF) were exposed to DLT (6.25 mg/kg) and CPF (4.75 mg/kg) (1/20th of the previously determined LD50 of 125 mg/kg and 95 mg/kg, respectively, over a period of 48 h). Rats in group V (CPF + DLT) were co-exposed to CPF (4.75 mg/kg) and DLT (6.25 mg/kg), while those in group VI (ALA + CPF + DLT) were pretreated with ALA (60 mg/kg) and then co-exposed to CPF and DLT, 45 min later. The treatments were administered by gavage once daily for a period of 16 weeks. Blood collected at the end of the experimental period were analyzed for erythrocyte osmotic fragility and malondialdehyde (MDA) concentration. The study showed that chronic co-exposure to CPF and DLT resulted in an increase in erythrocyte fragility and MDA concentration which were ameliorated by supplementation with alpha-lipoic acid. The study concluded that repeated co-exposure to CPF and DLT elevated erythrocyte fragility probably due to increased lipid peroxidation, and pretreatment with alpha-lipoic acid ameliorated these alterations.

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Section: Discussionsupporting

confidence: 93%

Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

et al. 2014

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“…Thus, accumulation of ROS exceeds the capacity of the antioxidant system (Chargui et al 2010) and therefore can produce cytotoxic effects (Rezg et al 2008b). Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, recent evidence suggests that exposure to different stress models and concentrations has an important implication in the alteration of the antioxidant defense system (Sahin et al 2004;Chargui et al 2010). Other systems that may be affected by OP pesticide exposure include the immune system (Handy et al 2002), the pancreas (Gokalp et al 2005;Gokcimen et al 2007), the kidney (Kalender et al 2007), the hematological system (Kalender et al 2006;Durak et al 2009), the reproductive system ), and the liver (Kalender et al 2005).…”

mentioning

confidence: 96%

Malathion-induced hepatotoxicity in male Wistar rats: biochemical and histopathological studies

Flehi-Slim

Chargui

Boughattas

et al. 2015

Environ Sci Pollut Res

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The increasing use of organophosphorus pesticides in the environment constitutes an ecotoxicological hazard especially for humans and non-target animals. Hereby, we analyzed the toxic effects of malathion on the histological structure of liver and biochemical parameters in male rats. Three groups received daily different amounts of malathion: 1/1000, 1/100, and 1/10 LD50 for 30 days. The weights of treated rat's liver have increased. Analyzed tissues showed centrilobular and sinusoidal congestion, hepatocyte hypertrophy, cellular vacuolization, anucleated hepatocytes, depletion of organelles affecting the majority of cells, and presence of necrotic foci into the hepatic parenchyma. Histological sections of the liver showed important hepatocyte glycogen storage. We conclude that malathion stimulates the filing of glycogen in a dose-dependent manner. Biochemical parameters showed that alanine transaminase (ALT), aspartate transaminase (AST), gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) levels increased in the treated groups when the level of total protein decreased in intoxicated groups.

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“…Increased oxidative damage due to high levels of ROS and LPO was detected in rat liver treated with chlorpyrifos and/or DEL at low (5 mg kg −1 day −1 ) and high (35 mg kg −1 day −1 ) doses for 16 weeks (Tuzmen et al, 2008). The mixture of DEL (0.003, 0.03, and 0.3 mg kg −1 day −1 ) and permethrin (0.013, 0.13, and 1.3 mg kg −1 day −1 ) for 30, 45, and 60 days decreased superoxide dismutase, GPx, and catalase activities in rats (Chargui et al, 2010). Decreased GST and increased MDA levels were found in rat liver treated with DEL (1.28 mg kg −1 day −1 ), fluoride (44.5 mg L −1 day −1 ), and their combination for 28 days (Dubey et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Co-exposure to deltamethrin and thiacloprid induces cytotoxicity and oxidative stress in human lung cells

2020

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Deltamethrin (DEL) and thiacloprid (THIA) are commonly used synthetic insecticides in agriculture either separately or in combination. There is limited information in human cells for the effects of the mixture of DEL + THIA on oxidative stress. Therefore, the present study was designed to examine the effects of the mixture on cell proliferation and oxidative stress in human lung fibroblast cells. Human telomerase reverse transcriptase (hTERT)-expressing human lung fibroblasts, WTHBF-6 cells, were treated with 2.5 + 37.5, 5 + 75, 12.5 + 187.5, and 25 +375 µM concentrations of DEL + THIA for the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and 5 + 75, 12.5 + 187.5, and 25 + 375 µM for lipid peroxidation and reduced glutathione (GSH) assays for 24, 48, and 72 h in the absence and presence of metabolizing fractions of the mammalian liver (S9 mixture). Both the mixture of DEL + THIA and their metabolites significantly reduced cell viability and induced cytotoxicity in WTHBF-6 cells, especially at higher concentrations. The mixture of DEL + THIA significantly decreased GSH levels at the highest concentration for all treatment times and at the highest two concentrations (12.5 + 187.5 and 25 + 375 µM) for 72 h in the presence of S9 mixture. The highest concentration of DEL + THIA mixture caused a significant increase in malondialdehyde (MDA) level at 72 h in the absence of S9 mixture. There were also significant increases in MDA levels at the highest concentration for 48-h and all concentrations of DEL + THIA for 72-h treatment in WTHBF-6 cell cultures with S9. These data showed that the mixture of DEL + THIA and their metabolites can induce cytotoxicity and oxidative stress in human lung fibroblasts.

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Erythrocyte antioxidants enzymes imbalance following subcutaneous pyrethroid treatments in rats of different sex

Cited by 11 publications

References 20 publications

Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

Malathion-induced hepatotoxicity in male Wistar rats: biochemical and histopathological studies

Co-exposure to deltamethrin and thiacloprid induces cytotoxicity and oxidative stress in human lung cells

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