Erwinia
            
            typographi sp. nov., isolated from bark beetle (Ips typographus) gut

Skrodenytė-Arbačiauskienė, Vesta; Radžiūtė, Sandra; Stunžėnas, Virmantas; Būda, Vincas

doi:10.1099/ijs.0.030304-0

Cited by 33 publications

(21 citation statements)

References 35 publications

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“…Carbon-source utilization was observed in each well by five OD 590 measurements taken between 15 and 48 h after bacterial strains had been incubated in the plate at 28 °C (Table S4). The data we obtained for the type strains of E. toletana , E. oleae , E. amylovora , E. aphidicola and E. typographi () matched their original descriptions (Harada et al , 1997; Hauben et al , 1998; Rojas et al , 2004; Moretti et al , 2011; Skrodenyte-Arbaciauskiene et al , 2012). Three of the four novel strains (including the type strain B120 T ) exhibited the same profile for all tests.…”

supporting

confidence: 64%

“…Of the bacteria isolated from aphid bodies or artificial diets or wheat after feeding by aphids, we found a subset of isolates that belong to the Erwinia – Pantoea clade. While members of the genera Erwinia and Pantoea are most commonly epiphytic or plant-pathogenic bacteria (Martinec & Kocur, 1964; Brady et al , 2010b), various strains of these genera are also known to associate with insects (Garcia-Salazar et al , 2000; Hildebrand et al , 2000; Wells et al , 2002; Gitaitis et al , 2003; Ellers-Kirk & Fleischer, 2006; Estes et al , 2009; Stavrinides et al , 2010; Skrodenyte-Arbaciauskiene et al , 2012), and especially aphids (Hemiptera: Aphididae) (Plurad et al , 1965; Harada et al , 1997; Capuzzo et al , 2005; Clark et al , 2012; Bansal et al , 2014). The aim of the present work was to characterize the Erwinia – Pantoea isolates associated with D. noxia and released during feeding.…”

mentioning

confidence: 99%

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Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Campillo

Luna

Portier

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia) Short, Gram-negative-staining, rod-shaped bacteria were isolated from crushed bodies of Russian wheat aphid [Diuraphis noxia (Kurdjumov)] and artificial diets after Russian wheat aphid feeding. Based on multilocus sequence analysis involving the 16S rRNA, atpD, infB, gyrB and rpoB genes, these bacterial isolates constitute a novel clade in the genus Erwinia, and were most closely related to Erwinia toletana. Representative distinct strains within this clade were used for comparisons with related species of Erwinia. Phenotypic comparisons using four distinct strains and average nucleotide identity (ANI) measurements using two distinct draft genomes revealed that these strains form a novel species within the genus Erwinia. The name Erwinia iniecta sp. nov. is proposed, and strain B120 T (5CFBP 8182 T 5NCCB 100485 T ) was designated the type strain. Erwinia iniecta sp. nov. was not pathogenic to plants. However, virulence to the Russian wheat aphid was observed.Aphids are sap-sucking insects. They penetrate plant tissues using a special mouthpart -the stylet -and they probe between the plant cell layers to find sieve elements. Once stylets penetrate the sieve elements, they alternately inject saliva and suck the plant sap (for a review, see Miles, 1999). This process for obtaining nutrients establishes an intimate and long-term interaction between the aphids and the host plant. In a few cases, aphids are proposed to vector or deliver plant-pathogenic bacteria into the plants via their stylets (Plurad et al., 1965;Watanabe et al., 1996;Stavrinides et al., 2009).In a previous study, we repeatedly and consistently isolated members of the Enterobacteriaceae from sterile artificial diets (15 % aqueous sucrose, pH 7.2) fed on by Russian wheat aphids [Diuraphis noxia (Kurdjumov)] (T. Campillo, L. van Eck, E. Luna, N. Lapitan, N. Tisserat and J. E. Leach, unpublished results). The bacteria were isolated only from artificial diets exposed to probing of D. noxia sylets through a Parafilm membrane; no bacteria were isolated from sterile diets that had not been exposed to aphids. In addition, bacteria were isolated from crushed aphid bodies and from wheat exposed to D. noxia feeding. Based on 16S rRNA gene sequences of 30 diverse isolates from these various origins, most of the bacteria grouped within the Enterobacteriaceae.Of the bacteria isolated from aphid bodies or artificial diets or wheat after feeding by aphids, we found a subset of isolates that belong to the Erwinia-Pantoea clade. While members of the genera Erwinia and Pantoea are most commonlyAbbreviations: ANI, average nucleotide identity; MLSA, multilocus sequence analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains B120 T , B137, B149 and B150 are KM870781-KM870784, respectively. The accession numbers for the draft genome sequences of B120 T and B149 are JRXF00000000 and JRXE00000000, respectively.Four supplementary tables and a supplementary figur...

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supporting

confidence: 64%

mentioning

confidence: 99%

Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Campillo

Luna

Portier

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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“…4 ). The exceptions are E. tracheiphila and E. typographi , an uncharacterized strain isolated from specialist bark beetles ( Skrodenytė-Arbačiauskienė et al 2012 ) ( fig. 4 ).…”

Section: Resultsmentioning

confidence: 99%

Horizontal Gene Acquisitions, Mobile Element Proliferation, and Genome Decay in the Host-Restricted Plant PathogenErwinia Tracheiphila

et al. 2016

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Modern industrial agriculture depends on high-density cultivation of genetically similar crop plants, creating favorable conditions for the emergence of novel pathogens with increased fitness in managed compared with ecologically intact settings. Here, we present the genome sequence of six strains of the cucurbit bacterial wilt pathogen Erwinia tracheiphila (Enterobacteriaceae) isolated from infected squash plants in New York, Pennsylvania, Kentucky, and Michigan. These genomes exhibit a high proportion of recent horizontal gene acquisitions, invasion and remarkable amplification of mobile genetic elements, and pseudogenization of approximately 20% of the coding sequences. These genome attributes indicate that E. tracheiphila recently emerged as a host-restricted pathogen. Furthermore, chromosomal rearrangements associated with phage and transposable element proliferation contribute to substantial differences in gene content and genetic architecture between the six E. tracheiphila strains and other Erwinia species. Together, these data lead us to hypothesize that E. tracheiphila has undergone recent evolution through both genome decay (pseudogenization) and genome expansion (horizontal gene transfer and mobile element amplification). Despite evidence of dramatic genomic changes, the six strains are genetically monomorphic, suggesting a recent population bottleneck and emergence into E. tracheiphila’s current ecological niche.

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“…For instance, bacterial isolates from the coniferous subcortical habitat such as Pseudomonas , Serratia, and Rahnella that are also associated with Dendroctonus valens reported reducing and metabolizing the monoterpenes 35 , 36 . Similarly, Erwinia typography obtained from IT gut ( Ips typographus ) showed tolerance to high concentration of spruce monoterpene myrcene 41 . Recently, using the metagenomic approach, bacterial community harbouring genes associated with terpene degradation were identified from mountain pine beetles 16 .…”

Section: Introductionmentioning

confidence: 85%

Unravelling the gut bacteriome of Ips (Coleoptera: Curculionidae: Scolytinae): identifying core bacterial assemblage and their ecological relevance

Chakraborty

Ashraf

Modlinger

et al. 2020

Sci Rep

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Bark beetles often serve as forest damaging agents, causing landscape-level mortality. Understanding the biology and ecology of beetles are important for both, gathering knowledge about important forest insects and forest protection. Knowledge about the bark beetle gut-associated bacteria is one of the crucial yet surprisingly neglected areas of research with European tree-killing bark beetles. Hence, in this study, we survey the gut bacteriome from five Ips and one non-Ips bark beetles from Scolytinae. Results reveal 69 core bacterial genera among five Ips beetles that may perform conserved functions within the bark beetle holobiont. The most abundant bacterial genera from different bark beetle gut include Erwinia, Sodalis, Serratia, Tyzzerella, Raoultella, Rahnella, Wolbachia, Spiroplasma, Vibrio, and Pseudoxanthomonas. Notable differences in gut-associated bacterial community richness and diversity among the beetle species are observed. Furthermore, the impact of sampling location on the overall bark beetle gut bacterial community assemblage is also documented, which warrants further investigations. Nevertheless, our data expanded the current knowledge about core gut bacterial communities in Ips bark beetles and their putative function such as cellulose degradation, nitrogen fixation, detoxification of defensive plant compounds, and inhibition of pathogens, which could serve as a basis for further metatranscriptomics and metaproteomics investigations.

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Erwinia typographi sp. nov., isolated from bark beetle (Ips typographus) gut

Abstract: Erwinia typographi sp. nov., isolated from bark beetle (Ips typographus) gut

Cited by 33 publications

References 35 publications

Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Horizontal Gene Acquisitions, Mobile Element Proliferation, and Genome Decay in the Host-Restricted Plant PathogenErwinia Tracheiphila

Unravelling the gut bacteriome of Ips (Coleoptera: Curculionidae: Scolytinae): identifying core bacterial assemblage and their ecological relevance

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