Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Campillo, Tony; Luna, Emily; Portier, Pérrine; Saux, Marion Fischer-Le; Lapitan, Nora L. V.; Tisserat, Ned; Leach, Jan E.

doi:10.1099/ijsem.0.000466

Cited by 24 publications

(24 citation statements)

References 40 publications

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“…T and E. iniecta B120 T , data were taken from the literature (Campillo et al, 2015;Skrodenytė-Arbačiauskienė et al, 2012). A dendrogram (Fig.…”

Section: Strains Em595mentioning

confidence: 99%

“…Multi-locus sequence analysis (MLSA) shows that the genus Erwinia is currently monophyletic for 17 accepted species, the majority of which are plant pathogens (Erwinia amylovora, E. pyrifoliae, E. piriflorinigrans, E. uzenensis, E. mallotivora, E. papayae, E. persicina, E. psidii, E. rhapontici and E. tracheiphila) (Hauben & Swings, 2005;Ló pez et al, 2011;Matsuura et al, 2012). However, the genus also encompasses epiphytes (E. billingiae and E. tasmaniensis) (Geider et al, 2006;Mergaert et al, 1999), species that may contribute to plant disease as part of multi-species microbial communities (E. toletana and E. oleae) (Buonaurio et al, 2015), and insect-associated bacteria (E. aphidicola, E. typographi and E. iniecta) (Campillo et al, 2015;Harada et al, 1997;Skrodenytė-Arbačiauskienė et al, 2012).…”

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confidence: 99%

“…S5. Biochemical data for E. gerundensis, E. uzenensis and E. piriflorinigrans were inferred in this study, those of other Erwinia species were taken from the literature (Skrodenyte-Arbaciauskiene et al, 2012; Campillo et al, 2015;Moretti et al, 2011 A phylogenomic tree based on the core genomes of strain EM595 T and related members of the genera Erwinia and Pantoea (Fig. 3) was reconstructed using EDGAR software (Blom et al, 2009).…”

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confidence: 99%

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Erwinia gerundensis sp. nov., a cosmopolitan epiphyte originally isolated from pome fruit trees

Rezzonico

Smits

Born

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A survey to obtain potential antagonists of pome fruit tree diseases yielded two yellow epiphytic bacterial isolates morphologically similar to Pantoea agglomerans, but showing no biocontrol activity. Whole-cell MALDI-TOF mass spectrometry and analysis of 16S rRNA gene and gyrB sequences suggested the possibility of a novel species with a phylogenetic position in either the genus Pantoea or the genus Erwinia. Multi-locus sequence analysis (MLSA) placed the two strains in the genus Erwinia and supported their classification as a novel species. The strains showed general phenotypic characteristics typical of this genus and results of DNA-DNA hybridizations confirmed that they represent a single novel species. Both strains showed a DNA G+C content, as determined by HPLC, of 54.5 mol% and could be discriminated from phylogenetically related species of the genus Erwinia by their ability to utilize potassium gluconate, potassium 2-ketogluconate, maltose, melibiose and raffinose. Whole-genome sequencing of strain EM595 T revealed the presence of a chromosomal carotenoid biosynthesis gene cluster similar to those found in species of the genera Cronobacter and Pantoea that explains the pigmentation of the strain, which is atypical for the genus Erwinia. Additional strains belonging to the same species were recovered from different plant hosts in three different continents, revealing the cosmopolitan nature of this epiphyte. Abbreviations: MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; MLSA, multi-locus sequence analysis.The GenBank/EMBL/DDBJ accession numbers for the gene sequences of strains EM486 and EM595T are FJ611847 and FJ611848 (16S rRNA), KP070832 and JN591389 (atpD), FJ617412 and FJ617413 (gyrB), KP070833 and KP070835 (infB), KP070834 and KP070836 (rpoB), respectively. The GenBank/EMBL/DDBJ accession numbers for the gyrB sequences of additional strains (6

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“…T and E. iniecta B120 T , data were taken from the literature (Campillo et al, 2015;Skrodenytė-Arbačiauskienė et al, 2012). A dendrogram (Fig.…”

Section: Strains Em595mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Erwinia gerundensis sp. nov., a cosmopolitan epiphyte originally isolated from pome fruit trees

Rezzonico

Smits

Born

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…These are both plant pathogens such as Erwinia papayae (Gardan et al, 2004), Erwinia piriflorinigrans (Ló pez et al, 2011) and Erwinia uzenensis (Matsuura et al, 2012), as well as non-pathogens or those, so far, not reported to be plant pathogens, such as Erwinia toletana (Rojas et al, 2004), Erwinia tasmaniensis (Geider et al, 2006) and Erwinia oleae (Moretti et al, 2011). Also, strains not associated with plants have been classified to novel species within the genus Erwinia, as with the recently described species Erwinia typographi, which was isolated from the gut of bark beetles in Lithuania (Skrodenytė-Arbačiauskienė et al, 2012) and Erwinia iniecta, isolated from wheat aphids in Russia (Campillo et al, 2015).…”

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Erwinia endophytica sp. nov., isolated from potato (Solanum tuberosum L.) stems

Ramírez-Bahena

Salazar

Cuesta

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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We analysed, using a polyphasic taxonomic approach, two bacterial strains coded BSTT30 T and BSTT40, isolated in the course of a study of endophytic bacteria occurring in the stems and roots of potatoes growing in soil from Salamanca, Spain. The 16S rRNA gene sequence was identical in both strains and had 98.4 % identity with respect to the closest relatives Erwinia tasmaniensis Et1/99T and Erwinia rhapontici ATCC29283T . Erwinia billingiae E63T and Erwinia toletana A37 T were also closely related with 98.2 % sequence similarities, so the novel strains were classified within the genus Erwinia. The analysis of the housekeeping genes gpd, gyrB and rpoD confirmed the phylogenetic affiliation of strains BSTT30 T and BSTT40with similarities of lower than 90 % in all cases with respect to the closest relatives mentioned above. The respiratory quinone of strain BSTT30 T was Q8. The major fatty acids were C16 : 0, C16 : 1v7c/16 : 1v6c in summed feature 3 and C18 : 1v7c/18 : 2v6,9c in summed feature 8. The novel strains were oxidase-negative and catalase-positive. Glucose was fermented without gas production. They were negative for arginine dihydrolase, urease and indole production. The strains could grow at 35 8C and at pH 10. DNA G+C content was 50.1 mol%. DNA-DNA hybridization results showed values of lower than 29 % relatedness with respect to the type strains of the four most closely related species. Therefore, the combined genotypic, phenotypic and chemotaxonomic data support the classification of strains BSTT30 T and BSTT40 into a novel species of the genus Erwinia, for which the name Erwinia endophytica sp. nov. is proposed. The type strain is BSTT30 T (5LMG 28457 T , CECT 8692 T ).

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“…Its presence in the sampled aphids might just reflect their acquisition form the host plant during feeding. Although two species of this genus, E. aphidicola and E. iniecta, have been described to negatively affect the infected aphid (Harada et al 1997, Campillo et al 2015, it is not clear whether Erwinia acts as an aphid pathogen, commensal, mutualist, it uses aphids as alternative hosts to plants, or it is just acquired during aphid feeding, so that the aphid acts as a vector for the plant pathogen.…”

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confidence: 99%

Natural Occurrence of Secondary Bacterial Symbionts in Aphids from Tunisia, with a Focus on Genus Hyalopterus

Zouari

Halima

Reyes-Prieto

et al. 2018

Environmental Entomology

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Aphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identified among many important pest aphid species. To determine interpopulational diversity of S-symbionts, we carried out a survey in a total of 18 populations of six aphid species collected from six localities in Tunisia, by performing a diagnostic polymerase chain reaction analysis of partial 16S-23S rRNA operon sequences. While 61.7% of individuals contained only Buchnera, three S-symbionts were found at different frequencies. Arsenophonus sp. Gherna et al. 1991 (Enterobacteriales: Enterobacteriaceae) was found in all species under study except for Acyrtosiphon pisum (Harris 1776) (Aphidinae: Macrosiphini); Serratia symbiotica Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was present in all analyzed individuals of A. pisum but only sporadically in Aphis spiraecola (Patch 1914) (Aphidinae: Aphidini) and Hyalopterus amygdali (Blanchard 1840) (Aphidinae: Aphidini), while Hamiltonella defensa Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was found in all analyzed individuals of one population of Aphis gossypii (Glover 1877) (Aphidinae: Aphidini) and sporadically in two populations of Hyalopterus. The lysogenic bacteriophage APSE-1 (A. pisum secondary endosymbiont, type 1) was detected in the three populations infected with H. defensa. This bacteriophage has been associated with moderate protection against braconid parasitoids in pea aphids. The high prevalence of Arsenophonus sp. in our samples is in accordance with previous studies indicating that, among gammaproteobacteria, this genus is one of the most widespread insect facultative symbionts.

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Erwinia iniecta sp. nov., isolated from Russian wheat aphid (Diuraphis noxia)

Cited by 24 publications

References 40 publications

Erwinia gerundensis sp. nov., a cosmopolitan epiphyte originally isolated from pome fruit trees

Erwinia gerundensis sp. nov., a cosmopolitan epiphyte originally isolated from pome fruit trees

Erwinia endophytica sp. nov., isolated from potato (Solanum tuberosum L.) stems

Natural Occurrence of Secondary Bacterial Symbionts in Aphids from Tunisia, with a Focus on Genus Hyalopterus

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