Erucic acid oxidation by beating heart cells in culture

Pinson, A; Padieu, P

doi:10.1016/0014-5793(74)80023-2

Cited by 36 publications

(15 citation statements)

References 9 publications

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“…The linear increase in tissue protein that occurred during a 2 wk incubation period was SUl6ported by the earlier observations of Orloff and McCarl (24) and Pinson (8), but differed from the reported growth characteristics of heart cells following myoblastic enrichment (7,22). The present findings are in conflict with earlier data (8) which suggested that erucic acid may enhance protein synthesis by rat heart cells in culture.…”

Section: Discussioncontrasting

confidence: 98%

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Erucic acid and phospholipids of newborn rat heart cells in culture

Rogers

1977

Lipids

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Erucic acid (delta 13-docosenoic acid), labeled with 14C in the 1- or 14-position, was incorporated into fetal calf serum and fed to beating, neonatal rat myocardial cell in culture. Uptake of the docosenoic acid during the first 6 hr of incubation was 41 nM/hr/mg protein in 7-day old cells and 29 nM/hr/mg protein in 14-day old cells. Fifty-seven percent of the 14C-activity was taken up from the medium in 24 hr, of which 77% was in the cells and 23% was unaccounted for. Of the 14C-activity taken up, 26% was in extractable lipid, with two-thirds in neutral lipid and one-third in phospholipid. Within the neutral lipid fraction, 88% of the 14C-activity was present in triglycerides; while in phospholipids, 66% of the 14C-activity was in phosphatidylcholine (PC); 14% in phosphatidylethanolamine (PE); 6% in sphinogomyelin (SPH) and 1% or less in cardiolipin (DPG). PC had the highest specific activity, followed by SPH and PE. The specific activity of PE was one-half that of SPH when the 14C-erucic acid substrate was labeled at the carboxyl position, but increased to equal that of SPH when the substrate was labeled at the double bond. The fatty acids of PC, PE, and SPH were influenced by erucic acid in the growth medium, but the amounts of each phospholipid were not affected. It is proposed that the altered fatty acid composition associated with incorporation of erucic acid or its metabolites into PC, PE, and SPH may affect integrity and function of heart cell membranes.

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Section: Discussioncontrasting

confidence: 98%

“…Pinson and Padieu (22) showed that rat heart cells in culture can rapidly metabolize 14C-erucic acid with the release of 14CO2. In other work (7), less than 10% of the total lipid 14C-activity was detected in phospholipids of 7-day old cells.…”

Section: Discussionmentioning

confidence: 99%

Erucic acid and phospholipids of newborn rat heart cells in culture

Rogers

1977

Lipids

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“…In the liver, the level of 20:1 observed was greater than that of 22:1, which suggests a rapid j3-oxidation of 22:1 to 20:1, etc. in the pig liver similar to that found in rats (17,18). Walker (3) reported equal concentrations of these two fatty acids after a 6-week experiment (2.9 % of 20:1, 2.5 % of 22:1), and Molnar et al (2) reported higher levels of 22:1 (7.6%) than 20:1 (1.5%) after a 5-week experi ment with pigs fed diets containing 10% by weight RSOs very similar in fatty acid composition.…”

Section: Discussionsupporting

confidence: 70%

Lipid Changes in Tissues of Young Boars Fed Rapeseed Oil or Corn Oil

Kramer¹,

Friend²,

Hulan³

1975

Ann Nutr Metab

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Fully refined rapeseed oil, Brassica campestris var. Arlo plus Echo, and corn oil were fed at 20 % by weight of a basal diet to young Yorkshire boars. A serial slaughter technique was employed whereby 3 boars from each dietary treatment were killed at 0, 1, 2, 3, 4 and 8 weeks of the experiment. The fatty acid profiles of heart, liver, adrenals and testes lipids were investigated. The typical fatty acids of rapeseed oil, erucic and eicosenoic acids, were incorporated at different levels into the tissue lipids examined. After feeding the rapeseed oil diet for 1 week, the relative concentration of these fatty acids increased in the adrenals, while remaining similar in the heart, liver and testes. The relative concentration of eicosenoic acid was greater than that of erucic acid in all organs examined, except for the heart which contained equal amounts of these acids. Tissue levels of these acids in boars were compared to those found in rats fed the same rapeseed oil in previous studies.

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“…The hepatrc cham shortenmg system may explain why rapeseed orl feeding does not induce fatty liver. A relatively slow cham shortening of erucrc acid in cultured heart myocytes has been described [6].…”

Section: Introductionmentioning

confidence: 99%