Erucic acid metabolism in rat heart. A combined biochemical and radioautographical study

Caselli, C.; Carlier, H.; Bézard, J.

doi:10.3109/13813459009114000

Cited by 2 publications

(13 citation statements)

References 44 publications

(23 reference statements)

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“…From the same experiment, results observed in heart cells and already published (CASELLI et al, 1990) showed that peroxisomes did not seem more implicated than mitochondria in erucic acid catabolism in the myocardium. Apparentely the observations reported here on liver cells confirm this point of view, but do not suggest any other interpretation.…”

Section: Introductionsupporting

confidence: 82%

“…After injection of labelled erucic or oleic acid, radioactivity recovered in liver lipids was relatively high with both fatty acids, expectedly much more than in heart lipids in the same experiment (CASELLI et al, 1990). It was of the same order of magnitude than that previously observed in the same kind of experiment (ONG et al, 1977;CLOUET et al, 1980).…”

Section: Discussionsupporting

confidence: 49%

“…The apparent low rate of erucic acid esterification has been already well documented in liver and in other organs (BOUCROT & BEZARD, 1973 al., 1986, 1987). It is not demonstrated that microsomal activation and esterification of the very long-chain erucic acid would be slower than for the shorter oleic acid (MANNAERTS et al, 1979;KRISANS et al, 1980;SINOH et al, 1987;CASELLI et al, 1990). The main reason could merely be the very low rate of mitochondrial activation and oxidation of erucic acid (BLOND et al, 1975;CHRISTOPHERSEN et al, 1982;BREMER & OSMIJNDSEN, 1984).…”

Section: Discussionmentioning

confidence: 92%

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Erucic acid metabolism in rat liver a combined biochemical and radioautographical study

Caselli¹,

Carlier²,

Bézard

1992

Archives Internationales de Physiologie, de Biochimie et de Bio

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Metabolism of erucic acid was studied in rat liver in comparison with oleic acid in relation with diet lipids. Rats were fed for 3 or 60 days a balanced diet containing 30% of the calories of either rapeseed oil rich in erucic acid or sunflower seed oil rich in linoleic acid. They were intravenously injected with tritiated erucic or oleic acid. After 1 or 15 min, the radioactivity recovered in liver lipids was 9 to 26% whatever the diet or the acid injected. One minute after injection of erucic acid a high part of radioactivity was recovered in the free fatty acid fraction and as untransformed erucic acid. After 15 min the major part of radioactivity was recovered in the triacylglycerol fraction which contained a high proportion of labelled oleic acid formed by shortening of erucic acid. The autoradiography did not show any marked difference between the labelling of peroxisomes and mitochondria when tritiated erucic or oleic acid was injected. These data do not bring about arguments for a peroxisomal nor for a mitochondrial location of erucic acid shortening in liver.

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Section: Introductionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 92%

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Erucic acid metabolism in rat liver a combined biochemical and radioautographical study

Caselli¹,

Carlier²,

Bézard

1992

Archives Internationales de Physiologie, de Biochimie et de Bio

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“…Similar results are reported in isolated organs [22][23][24][25], and in intact animals [26][27][28]. Although 22:1n-9 is poorly oxidized to CO 2 [29,30], it is quickly converted into oleic acid (18:1n-9) in vivo and in isolated tissues and cultured cells [15,18,19,24,[26][27][28][31][32][33][34][35]. This conversion is presumably through peroxisome localized b-oxidation [19,33,36], although a possible mitochondrial pathway for 22:1n-9 oxidation can not be excluded [26,27].…”

supporting

confidence: 89%

“…In cultured cells, 22:1n-9 is rapidly esterified into TAG and to a lesser extent into PL pools [18][19][20][21]. Similar results are reported in isolated organs [22][23][24][25], and in intact animals [26][27][28]. Although 22:1n-9 is poorly oxidized to CO 2 [29,30], it is quickly converted into oleic acid (18:1n-9) in vivo and in isolated tissues and cultured cells [15,18,19,24,[26][27][28][31][32][33][34][35].…”

supporting

confidence: 68%

Erucic Acid is Differentially Taken up and Metabolized in Rat Liver and Heart

Murphy

Golovko

2008

Lipids

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Because X-linked adrenoleukodystrophy is treated using erucic acid (22:1n-9), we assessed its metabolism in rat liver and heart following infusion of [14-(14)C]22:1n-9 (170 Ci/kg) under steady-state-like conditions. In liver, 2.3-fold more tracer was taken up as compared to heart, accounted entirely by increased incorporation into the organic fraction (4.2-fold). The amount of tracer entering the aqueous fraction, which represents beta-oxidation, was not different between groups; however a significantly elevated proportion of tracer was in the heart aqueous fraction. In both tissues, 76% of the radioactivity found in the organic fraction was esterified in neutral lipids, while only about 10% was found esterified into phospholipids. In liver, 56% of lipid radioactivity was found in cholesteryl esters, whereas in heart 64% was found in triacylglycerols. Because 22:1n-9 can be chain shortened, we assessed tracer metabolism using phenacyl fatty acid derivatives esterified from saponified esterified neutral lipid (triacylglycerol/cholesteryl ester) and phospholipid fractions. In heart esterified neutral lipids, 75% of tracer was recovered as 22:1n-9 and only 10% as oleic acid (18:1n-9), while in liver only 25% of the tracer was recovered as 22:1n-9, while 50% was found as stearic acid (18:0) and 10% as 18:1n-9. In liver and heart phospholipids, the tracer was distributed amongst the n-9 fatty acid family. Thus, 22:1n-9 under went tissue selective metabolism, with conversion to 18:0 the dominant pathway in the liver presumably for export in the neutral lipids, while in heart it was found primarily as 22:1n-9 in neutral lipids and used for beta-oxidation.

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Erucic acid metabolism in rat heart. A combined biochemical and radioautographical study

Cited by 2 publications

References 44 publications

Erucic acid metabolism in rat liver a combined biochemical and radioautographical study

Erucic acid metabolism in rat liver a combined biochemical and radioautographical study

Erucic Acid is Differentially Taken up and Metabolized in Rat Liver and Heart

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