Erucic Acid is Differentially Taken up and Metabolized in Rat Liver and Heart

Murphy, Cameron C.; Murphy, Eric J.; Golovko, Mikhail Y.

doi:10.1007/s11745-008-3168-3

Cited by 21 publications

(11 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recent information on the biological action of glucosinolates and erucic acid has suggested that these are not as detrimental as originally thought [4]. Rats are relatively slow at digesting erucic acid and due to its accumulation in the heart , thought to be cardiotoxic [35] but recent studies have shown that the animals will adjust to the feed and increase erucic acid metabolism within their peroxisomes [36]. Glucoinsolates were thought to be only anti-nutritional but now the research evidence has demonstrated that glucosinolates may act as a chemo-protective, anti-cancer agent [12].…”

Section: Discussionmentioning

confidence: 99%

Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

Meadus

Duff

McDonald

et al. 2014

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight.

show abstract

Section: Discussionmentioning

confidence: 99%

Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

Meadus

Duff

McDonald

et al. 2014

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…In the absence of sufficient fat from the diet, the body synthesizes fatty acids, typically from carbohydrates. An interesting observation has been made that in adult rat liver, erucic acid (22:1)—a fatty acid that has been associated with heart disease and that is present in rapeseed oil—is rapidly converted to 18:0, demonstrating the conservation of carbons by chain-shortening of a monounsaturated fatty acid to an unsaturated fatty acid [33]. Even though all fatty acids present in the diet can be broken down and resynthesized into saturated fats, they have discrete effects.…”

Section: Biological Activities Of Saturated Fatty Acidsmentioning

confidence: 99%

Saturated Fats: A Perspective from Lactation and Milk Composition

German

Dillard

2010

Lipids

View full text Add to dashboard Cite

For recommendations of specific targets for the absolute amount of saturated fat intake, we need to know what dietary intake is most appropriate? Changing agricultural production and processing to lower the relative quantities of macronutrients requires years to accomplish. Changes can have unintended consequences on diets and the health of subsets of the population. Hence, what are the appropriate absolute amounts of saturated fat in our diets? Is the scientific evidence consistent with an optimal intake of zero? If not, is it also possible that a finite intake of saturated fats is beneficial to overall health, at least to a subset of the population? Conclusive evidence from prospective human trials is not available, hence other sources of information must be considered. One approach is to examine the evolution of lactation, and the composition of milks that developed through millennia of natural selective pressure and natural selection processes. Mammalian milks, including human milk, contain 50% of their total fatty acids as saturated fatty acids. The biochemical formation of a single double bond converting a saturated to a monounsaturated fatty acid is a pathway that exists in all eukaryotic organisms and is active within the mammary gland. In the face of selective pressure, mammary lipid synthesis in all mammals continues to release a significant content of saturated fatty acids into milk. Is it possible that evolution of the mammary gland reveals benefits to saturated fatty acids that current recommendations do not consider?

show abstract

“…Incorporation of erucic acid into the liver was demonstrated in ALD patients who had been administered LO (Murphy et al 2008). As the bulk of plasma VLCFAs exists as neutral lipids in low density lipoprotein, it is likely that the fatty acid level in plasma is largely associated with lipid metabolism in the liver.…”

Section: Introductionmentioning

confidence: 99%

Effect of Lorenzo’s Oil on Hepatic Gene Expression and the Serum Fatty Acid Level in abcd1-Deficient Mice

et al. 2017

View full text Add to dashboard Cite

Lorenzo's oil is known to decrease the saturated very long chain fatty acid (VLCFA) level in the plasma and skin fibroblasts of X-linked adrenoleukodystrophy (ALD) patients. However, the involvement of Lorenzo's oil in in vivo fatty acid metabolism has not been well elucidated. To investigate the effect of Lorenzo's oil on fatty acid metabolism, we analyzed the hepatic gene expression together with the serum fatty acid level in Lorenzo's oiltreated wild-type and abcd1-deficient mice. The change in the serum fatty acid level in Lorenzo's oil-treated abcd1-defcient mice was quite similar to that in the plasma fatty acid level in ALD patients supplemented with Lorenzo's oil. In addition, we found that the hepatic gene expression of two peroxisomal enzymes, Dbp and Scp2, and three microsomal enzymes, Elovl1, 2, and 3, were significantly stimulated by Lorenzo's oil. Our findings indicate that Lorenzo's oil activates hepatic peroxisomal fatty acid boxidation at the transcriptional level. In contrast, the transcriptional stimulation of Elovl1, 2, and 3 by Lorenzo's oil does not cause changes in the serum fatty acid level. It seems likely that the inhibition of these elongation activities by Lorenzo's oil results in a decrease in saturated VLCFA. Thus, these results not only contribute to a clarification of the mechanism by which the saturated VLCFA level is reduced in the serum of ALD patients by Lorenzo's oiltreatment, but also suggest the development of a new therapeutic approach to peroxisomal b-oxidation enzyme deficiency, especially mild phenotype of DBP deficiency.

show abstract

Erucic Acid is Differentially Taken up and Metabolized in Rat Liver and Heart

Cited by 21 publications

References 69 publications

Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

Saturated Fats: A Perspective from Lactation and Milk Composition

Effect of Lorenzo’s Oil on Hepatic Gene Expression and the Serum Fatty Acid Level in abcd1-Deficient Mice

Contact Info

Product

Resources

About