Erratum to “Measurement of drug–protein binding by immobilized human serum albumin-HPLC and comparison with ultrafiltration” [J. Chromatogr. B 834 (2006) 108–116]☆

Amino acids, peptides, and proteins as chemically bonded stationary phases – A review

“…HSA was in situ covalently immobilized on a diol‐bonded silica gel that had been activated with 1,1‐carbonyldiimidazole. Chromatographic studies have shown that HSA‐CSP can be used for chiral separations of enantiomeric solutes and that these separations may reflect in vitro binding to the HSA . The HSA‐CSP exhibits comparable properties to the BSA‐CSP and it offers selectivity for e.g.…”

Simultaneous ligand fishing and identification of human serum albumin binders from Eucommia ulmoides bark using surface plasmon resonance-high performance liquid chromatography–tandem mass spectrometry

“…Up to now, SPR has been used as a new analytical device to fish out binders of drug targets from pure synthetic or natural molecules and natural product extracts for drug screening, such as protein tyrosine phosphatase 1b (PTP1B) binders [23], ␣-glucosidase binders [24], DNA binders [18], etc. Most ligands bind to HSA in a reversible manner by means of weak chemical bonds such as ionic, van der Waals, hydrogen and hydrophobic bonds with the hydroxyl, carboxyl or other reversible sites available in the amino acids of HSA [25]. Therefore, the purpose of this study was to explore the possibility of using weak affinity SPR biosensor to screen HSA binders from E. ulmoides bark extract.…”

Interaction of biocompatible natural rosin-based surfactants with human serum albumin: A biophysical study

“…This flexibility allows the protein to variety of ligands [25]. As conformational adaptability of HSA extends well beyond the immediate vicinity of the binding site(s), cooperativity and allosteric modulation arise among binding sites which makes HSA similar to a multimeric protein [37]. Therefore, studies on this aspect can provide information of the structural features that determine the therapeutic efficacy of drugs [38].…”