Erratum to “Gardenia herbal active constituents: Applicable separation procedures” [J. Chromatogr. B 812 (2004) 193–202]

Wang, Shau Chun; Tseng, Ting Yu; Huang, Chih Min; Tsai, Tung Hu

doi:10.1016/j.jchromb.2006.02.030

Cited by 6 publications

(11 citation statements)

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“…The molecular weight of peak 4 was 162 Da higher than that of geniposide, which corresponds to a glucose residue. Referenced to the reported literature,18, 21 and our experimental data above, peak 4 was deduced as genipin gentiobioside.…”

Section: Resultssupporting

confidence: 56%

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Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Ren¹,

Xue²,

Zhang³

et al. 2007

Rapid Comm Mass Spectrometry

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Fragmentation pathways of five iridoid glycosides have been studied by using electrospray ionization multi-stage tandem mass spectrometry (ESI-MS(n)). The first-stage MS data of the five iridoid glycosides were compared. The MS spectra showed that the adduct ions of iridoid glycosides and the formate anion were diagnostic ions to distinguish iridoid glycosides with a carboxyl group at the C-4 position or an ester group at the C-4 position. The MS fragmentation pathways of the five iridoid glycosides were also studied. Analyzing the product ion spectra of iridoid glycosides, some neutral losses were observed, such as H(2)O, CO(2) and glucose residues, which were very useful for the identification of the functional groups in the structures of iridoid glycosides. Furthermore, specific loss of one molecule of methyl 3-oxopropanoate or 3-oxopropanic acid was firstly discussed, which corresponded to the isomerization of the hemiacetal group in the structure of iridoid aglycone. According to the fragmentation mechanisms and HPLC/MS(n) data, the structures of five iridoid glycosides in a crude extract of Gardenia jasminoisdes fruit have been identified. Three compounds were compared with standards and the other two were identified as shanzhiside and genipin gentibioside by their MS(n) data without standard compounds. In order to further validate the veracity of the deduction, genipin gentiobioside was isolated from the extract of Gardenia jasminoisdes fruit using Purification Factory and was further identified by C- and H-NMR.

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Section: Resultssupporting

confidence: 56%

“…In the theory of traditional Chinese medicine, the medicine is bitter and cold and enters the heart, lungs and triple burner meridians 20. It contains a large amount of iridoid glycosides such as geniposide, gardenoside, genipin gentibioside, gardoside, shanzhiside, geniposidic acid, scandoside methyl ester and methyl deacetyl asperulosidate, inter alia 21…”

mentioning

confidence: 99%

Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Ren¹,

Xue²,

Zhang³

et al. 2007

Rapid Comm Mass Spectrometry

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“…Amongst these, the stationary phase is of primary importance and its selectivity is influenced by a series of factors. However, perhaps the most important motivator for the development of stationary phases having different selectivities is related to better separations of polar or easily polarizable compounds, which include the majority of basic compounds, using RP-HPLC conditions [41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Some new selective stationary phases for RP‐HPLC

Jardim

Maldaner

Lourenço

et al. 2010

J of Separation Science

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At the present time, more complex analyses of apolar compounds with similar chemical structures or of polar compounds, especially basic ones, having diverse properties require more selective stationary phases having better stabilities. This paper describes several new stationary phases with directed selectivities that were prepared by immobilizing two different phenyl group-containing siloxanes and a trifluoropropyl-containing siloxane onto chromatographic silica and, in the case of the fluorinated siloxane, onto zirconized silica, using thermal treatment or microware radiation. The chromatographic properties and stabilities of these new phases were determined and several applications were evaluated. The phenyl-containing phases showed excellent characteristics related to the separation of several different types of aromatic compounds while the fluorinated phases, which present a more polar character, revealed selectivity for the separation of positional isomers as well as for a mixture of basic pharmaceuticals. Stability tests indicate that immobilization of the polysiloxanes increases column lifetimes by making the stationary phases less susceptible to dissolution, while the phases immobilized with microwave radiation were somewhat more stable than those immobilized by thermal treatments.

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“…The HPLC methods used in the study for the separation of acidic and basic mixtures were based on those published by Wang et al [3,10]. Then mobile phase compositions were modified to adapt the tested compounds.…”

Section: Mobile Phasementioning

confidence: 99%

“…The monolithic packings consist of one piece of either an organic polymer or silica with a continuous homogenous phase instead of a packed bed with individual particles. This column is usually made by solgel technology, which enables formation of highly porous material, containing two types of pores (macropores and mesopores) in its structure [3]. The large pores (typically 2 lm) are responsible for a low flow resistance and therefore permit the application of high eluent flow rates, while the small pores (about 12 nm) verify sufficient surface area for separation efficiency [4].…”

Section: Introductionmentioning

confidence: 99%

Comparison between monolithic and particle‐packed platinum C₁₈columns in HPLC determination of acidic and basic test mixtures

Atia

York

Clark

2009

J of Separation Science

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Herein we report the results of a comparative study on the performance of Monolithic RP-18e and Platinum C(18) 3 mum columns for isocratic separation of acidic and basic test compounds. The inter- and intraday precision of different practical parameters including number of theoretical plates (N), capacity factor (K'), tailing factor (T(0.05)), and resolution (R(s)) were determined for both columns. Two different production batches were used for each column and batch to batch reproducibility of both columns was evaluated. The column backpressure drop over flow rate range 0.5-2 mL/min at the monolithic columns was two- to three-times lower than that on the platinum column without loss of the column efficiency. The plate heights were used to estimate the columns efficiency using Van Deemter plots. Both types of columns were able to separate the tested compounds well with sufficient resolution and peak symmetry but they differ in the analysis time and column backpressure, significantly. Monolithic column was more convenient as it enables the analytical run under low backpressure at shorter time with sufficient separation efficiency.

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Erratum to “Gardenia herbal active constituents: Applicable separation procedures” [J. Chromatogr. B 812 (2004) 193–202]

Cited by 6 publications

References 0 publications

Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Some new selective stationary phases for RP‐HPLC

Comparison between monolithic and particle‐packed platinum C₁₈columns in HPLC determination of acidic and basic test mixtures

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Erratum to “Gardenia herbal active constituents: Applicable separation procedures” [J. Chromatogr. B 812 (2004) 193–202]

Cited by 6 publications

References 0 publications

Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Studies of iridoid glycosides using liquid chromatography/electrospray ionization tandem mass spectrometry

Some new selective stationary phases for RP‐HPLC

Comparison between monolithic and particle‐packed platinum C18 columns in HPLC determination of acidic and basic test mixtures

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Product

Resources

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Comparison between monolithic and particle‐packed platinum C₁₈columns in HPLC determination of acidic and basic test mixtures