Erratum to Bacterial diversity in toxic Alexandrium tamarense blooms off the Orkney Isles and the Firth of Forth

Abstract

PSP toxin content were not accompanied by changes of DGGE band patterns. We therefore presume that the bacterial groups identified in this study were not exclusively associated with toxic A. tamarense, but were generally associated with the phytoplankton.

Bacterial community dynamics during a bloom caused by Akashiwo sanguinea in the Xiamen sea area, China

“…But, many studies (Grossart et al, 2005;Rooney-Varga et al, 2005;Wichels et al, 2004) have confirmed the reproducibility of PCR and DGGE gels. We assumed that a possible amplification bias for or against a certain sequence would be constant and that changes in relative band intensity would reflect actual changes in the relative abundance of a phylotype.…”

“…Filtration was conducted as soon as the samples were taken to the lab and filters were held at À70 8C until analysis. DNA extraction was performed as described in detail by Wichels et al (2004). Filters were washed using DNA extraction buffer (Tris 50 mmol L À1 , EDTA 20 mmol L À1 , NaCl 100 mmol L À1 , CTAB 2%, pH 8.0), and cell lysis was facilitated by adding lysozyme (2 mg mL À1 ) and SDS (10%).…”

Evolution and Distribution of Saxitoxin Biosynthesis in Dinoflagellates

“…This hypothesis has been investigated by numerous studies, though the results were conflicting [30,31,32,33,34]. Additionally, some of the methods used to measure bacterial metabolites have subsequently been shown to lack specificity, with compounds thought to be STX later being identified as chemical imposters [32,34].…”

Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense

“…There have been reports concerning the bacteria associated with laboratory maintained strains of A. tamarense (Kodama et al, 1996;Shimizu et al, 1996;Gallacher et al, 1997;Kopp et al, 1997;Babinchak et al, 1998;Hold et al, 2001a;Simon et al, 2002;Zheng et al, 2005) and also A. tamarense blooms (Wichels et al, 2004). These investigations mainly focused on the bacteria associated with laboratory cultures of A. tamarense, bacterial production of PSP and their effects on the PSP production of A. tamarense.…”