Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense

Su, Jian; Xiao, Yang; Zheng, Tian Ling; Tian, Yun; Jiao, N.; Cai, Li Zhe; Hong, Haizheng

doi:10.1016/j.hal.2007.04.004

Cited by 113 publications

(85 citation statements)

References 37 publications

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“…Several strains of bacteria that could inhibit or kill HAB species had been isolated (Kim et al 2008;Mayali and Azam 2004;Park et al 2010;Su et al 2007). However, few studies on actinomycetes with algicidal activity against HAB species have been reported to date.…”

Section: Discussionmentioning

confidence: 99%

“…The fluorescence density of P. globosa cultures was measured after 24 h of the above-described treatment. Algicidal activity was calculated as follows (Su et al 2007):…”

Section: Analysis Of Algicidal Activitymentioning

confidence: 99%

“…Bacterial cells from O4-6 cultures grown in AC1 broth were collected by centrifugation (5,000×g for 20 min). Extraction of genomic DNA from O4-6 and PCR amplification for the 16S rRNA gene were performed as described by Su (Su et al 2007). The PCR products were purified from agarose gel with a GeneClean Turbo Kit (Qbiogene) and ligated with a pMD 19-T vector; the ligation products were transformed into Escherichia coli DH5α competent cells, followed by sequencing, which was performed by Shanghai Invitrogen Biotechnology Co., Ltd.…”

Section: Analysis Of Algicidal Activitymentioning

confidence: 99%

“…Therefore, biological methods have received particular interest in recent years because of their potential effectiveness, species specificity, and ecofriendliness, although using bio-resources still has many logistic problems and is far from the application stage (Anderson 2009). Research into algal-bacterial interactions has resulted in the isolation of several strains of bacteria capable of inhibiting or killing HAB species (Kim et al 2008;Mayali and Azam 2004;Park et al 2010;Su et al 2007). In addition, most algicidal bacteria are specific to algal species, making their use to control HABs an environment-friendly approach.…”

Section: Introductionmentioning

confidence: 99%

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A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa

Zheng

Zhang

et al. 2012

Appl Microbiol Biotechnol

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A strain O4-6, which had pronounced algicidal effects to the harmful algal bloom causing alga Phaeocystis globosa, was isolated from mangrove sediments in the Yunxiao Mangrove National Nature Reserve, Fujian, China. Based on the 16S rRNA gene sequence and morphological characteristics, the isolate was found to be phylogenetically related to the genus Streptomyces and identified as Streptomyces malaysiensis O4-6. Heat stability, pH tolerance, molecular weight range and aqueous solubility were tested to characterize the algicidal compound secreted from O4-6. Results showed that the algicidal activity of this compound was not heat stable and not affected by pH changes. Residue extracted from the supernatant of O4-6 fermentation broth by ethyl acetate, was purified by Sephadex LH-20 column and silica gel column chromatography before further structure determination. Chemical structure of the responsible compound, named NIG355, was illustrated based on quadrupole time-of-flight mass spectrometry (Q-TOF-MS) and nuclear magnetic resonance (NMR) spectra. And this compound showed a stronger algicidal activity compared with other reported algicides. Furthermore, this article represents the first report of an algicide against P. globosa, and the compound may be potentially used as a bio-agent for controlling harmful algal blooms.

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Section: Discussionmentioning

confidence: 99%

“…The fluorescence density of P. globosa cultures was measured after 24 h of the above-described treatment. Algicidal activity was calculated as follows (Su et al 2007):…”

Section: Analysis Of Algicidal Activitymentioning

confidence: 99%

Section: Analysis Of Algicidal Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa

Zheng

Zhang

et al. 2012

Appl Microbiol Biotechnol

Self Cite

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“…The PCR procedure was carried out as follows. An initial denaturing period of 5 min was followed by 30 cycles at 94 C for 1 min, 55 C for 1 min, 72 C for 2 min, and a final extension (72 C) time of 10 min (Su et al, 2007). Then the PCR products were sent to Invitrogen Ltd. (Shanghai, China) for sequencing.…”

Section: Isolation and Identification Of Bacteriamentioning

confidence: 99%

High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3

Chen

Sun

et al. 2017

International Biodeterioration & Biodegradation

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a b s t r a c tA bacterial strain, CN-3, was isolated from petroleum-contaminated sediment in Bohai Bay, China. Morphological, physiological and phylogenetic analyses indicated that the strain belonged to the genus Dietzia. It was capable of utilizing a wide range of alkanes (C 14 eC 31 ), aromatic compounds and crude oil as the sole carbon and energy sources. The bacterium showed high degradation rate (>80%) of the saturated hydrocarbons of crude oil in the pH range of 6e9. The degradation rate was hardly affected at a NaCl concentration up to 85 g L À1 , indicating that the strain can tolerate high salinities. The real-time quantitative Polymerase Chain Reaction (PCR) results showed that the CYP153 genes could be significantly induced by C 14 , C 15 , C 16 , C 26 and pristane, whereas the transcription level of alkB gene was increased moderately with C 14 , C 15 , C 16 , C 26 and pristane induction condition. A high degradation extent, wide substrate range and facile conditions enhanced the potential for this bacterial strain to be used in the bioremediation of crude oil pollution.

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Anticyanobacterial process and action mechanism of Streptomyces sp. HJC‐D1 on Microcystis aeruginosa

Kong

Wang

Chen

et al. 2020

Env Prog and Sustain Energy

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Eutrophication of environmental waters and occurrence of harmful cyanobacterial blooms have drawn much attention all over the world. For controlling cyanobacteria overgrowth, biological control methods such as anticyanobacterial process by microorganisms have been recently explored. In this study, a Streptomyces strain named HJC‐D1 is isolated from an eutrophic pond in China, which exhibited anticyanobacterial activity against a representative bloom‐forming cyanobacterium Microcystis aeruginosa. Based on its morphology and 16S rRNA sequence, the isolated strain is identified as Streptomyces sp. Results show that the M. aeruginosa is biodegraded by the isolated strain HJC‐D1, and the Chl a removal efficiency is 53.5 ± 4.3%–62.6 ± 3.3% at the initial Chl a concentration of 0.2713–0.6375 mg L−1. The anticyanobacterial mechanism of Streptomyces sp. on M. aeruginosa is that the growth of cyanobacterial cell is destroyed by the active substances secreted by strain HJC‐D1, and TEM results demonstrate the degradation processes are divided into three steps: cell breakage, organelle release, and cell death. The isolated microorganism may be a potential environment‐friendly strain and provides a possible approach for Microcystis bloom control in lakes and reservoirs.

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Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense

Cited by 113 publications

References 37 publications

A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa

A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa

High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3

Anticyanobacterial process and action mechanism of Streptomyces sp. HJC‐D1 on Microcystis aeruginosa

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