Errata: Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Salehi, Hamideh; Middendorp, Elodie; Panayotov, Ivan; Collart-Dutilleul, Pierre-Yves; Vegh, Attila-Gergely; Ramakrishnan, Sathish; Gergely, Csilla; Cuisinier, Frederic

doi:10.1117/1.jbo.18.5.059801

Cited by 6 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This approach corroborates single‐cell Raman imaging published by other authors. [ 93,118,122 ] In Figure 17b, Raman spectra are shown in the wavenumber interval 600–1000 cm −1 as collected in the respective cell cytoplasm (cf. locations labeled with an asterisk on the maps in Figure 17a).…”

Section: Applications Of In Situ Raman Spectroscopy To Microbiologymentioning

confidence: 99%

Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

114

View full text Add to dashboard Cite

The Raman spectrum of living cells and microorganisms contains highly specific fingerprint‐like signatures, useful in unequivocally identifying different species and interpreting physiological and metabolic responses to environmental stressors. In situ Raman imaging with dedicated highly sensitive instruments can translate selected spectroscopic fingerprints into vivid snapshots of molecular species or specific physiological reactions. Time‐lapse experiments, crucial in characterizing growth‐dependent phenomena and metabolic response to drugs or substrates, are possible because Raman imaging is life compatible. This review covers miscellaneous examples of Raman analyses and imaging of eukaryotic cells, bacteria, and viruses. Fundamental microbiological analyses covered here include (i) identification of different species of cells, bacteria, and viruses; (ii) characterization of the metabolic responses of cells and bacteria to different substrates; (iii) time‐lapse analyses of cell metabolic reactions upon viral inoculation; (iv) chemical imaging of axon sprouting in neuronal cells; and (v) visualization of the myelinating activity of living Schwann cells in coculture with neuronal cells. The spectroscopic findings displayed here, which are based on a machine learning approach applied to Raman analysis and imaging, demonstrate the invaluable potential for Raman spectroscopy in biophysics research.

show abstract

Section: Applications Of In Situ Raman Spectroscopy To Microbiologymentioning

confidence: 99%

Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

114

View full text Add to dashboard Cite

show abstract

“…le Roux et al explored cell death routes after exposure to gold‐based metallodrugs. Salehi et al and Feofanov et al probed the resonance Raman response to detect and study cellular responses after exposure to paclitaxel, and the non‐fluorescent transition‐metal complex, theraphtal, respectively. Draux et al investigated the effect of gemcitabine on cell biomolecules in the lung cancer cell line Calu‐1, after 48‐hour exposure.…”

Section: Introductionmentioning

confidence: 99%

In vitro label‐free screening of chemotherapeutic drugs using Raman microspectroscopy: Towards a new paradigm of spectralomics

Farhane¹,

Nawaz

Bonnier

et al. 2018

Journal of Biophotonics

View full text Add to dashboard Cite

This overview groups some of the recent studies highlighting the potential application of Raman microspectroscopy as an analytical technique in preclinical development to predict drug mechanism of action and in clinical application as a companion diagnostic and in personalised therapy due to its capacity to predict cellular resistance and therefore to optimise chemotherapeutic treatment efficacy. Notably, the anthracyclines, doxorubicin and actinomycin D, elicit similar spectroscopic signatures of subcellular interaction characteristic of the mode of action of intercalation. Although cisplatin and vincristine show markedly different signatures, at low exposure doses, their signatures at higher doses show marked similarities to those elicited by the intercalating anthracyclines, confirming that anticancer agents can have different modes of action with different spectroscopic signatures, depending on the dose. The study demonstrates that Raman microspectroscopy can elucidate subcellular transport and accumulation pathways of chemotherapeutic agents, characterise and fingerprint their mode of action, and potentially identify cell-resistant strains. The consistency of the spectroscopic signatures for drugs of similar modes of action, in different cell lines, suggests that this fingerprint can be considered a "spectralome" of the drug-cell interaction suggesting a new paradigm of representing spectroscopic responses.

show abstract

“…Data analysis shows no release of cytochrome c in cytoplasm. Cytochrome c release is observed during cell death . At the end of experiments, the MCF7 cells are still adherent on CaF 2 substrate, and no visual damage was observed.…”

Section: Methodsmentioning

confidence: 93%

Confocal Raman spectroscopy to monitor intracellular penetration of TiO₂ nanoparticles

Salehi¹,

Calas-Bennasar²,

Durand³

et al. 2014

J Raman Spectroscopy

Self Cite

View full text Add to dashboard Cite

Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells. The penetration of TiO2 nanoparticles into cells revealed a gradual time‐dependent diffusion of nanoparticles over the entire cell. Cell apoptosis was monitored by tracing cytochrome c diffusion into the cytoplasm. A comparison with the mitochondrial clustering indicated that cytochrome c was inside the mitochondria for TiO2 concentration of 2 µg ml−1. This result demonstrates that the presence of TiO2 particles within a cell does not induce apoptosis. We demonstrated that confocal Raman microscopy allow to follow penetration of TiO2 particles in cell and to monitor the apoptotic status of the penetrated cells. Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

Errata: Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Cited by 6 publications

References 0 publications

Raman spectroscopy in cell biology and microbiology

Raman spectroscopy in cell biology and microbiology

In vitro label‐free screening of chemotherapeutic drugs using Raman microspectroscopy: Towards a new paradigm of spectralomics

Confocal Raman spectroscopy to monitor intracellular penetration of TiO₂ nanoparticles

Contact Info

Product

Resources

About

Errata: Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Cited by 6 publications

References 0 publications

Raman spectroscopy in cell biology and microbiology

Raman spectroscopy in cell biology and microbiology

In vitro label‐free screening of chemotherapeutic drugs using Raman microspectroscopy: Towards a new paradigm of spectralomics

Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles

Contact Info

Product

Resources

About

Confocal Raman spectroscopy to monitor intracellular penetration of TiO₂ nanoparticles