Confocal Raman spectroscopy to monitor intracellular penetration of TiO<sub>2</sub> nanoparticles

Salehi, Hamideh; Calas-Bennasar, Isabelle; Durand, Jean‐Olivier; Middendorp, Elodie; Valcárcel, J.; Larroque, Christian; Nagy, Katalin; K, Kinga Turzó; Dékány, Imre; Cuisinier, Frédéric

doi:10.1002/jrs.4561

Cited by 12 publications

(12 citation statements)

References 53 publications

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“…The path of paclitaxel (PTX), a classical anticancer drug, transported by DPSCs and absorbed by MCF-7 cells, was successfully monitored by means of confocal Raman microscopy. This label-free imaging method enabled detection of the cellular organelles (mitochondria), biological molecules, such as cytochrome c, and also tracing of the drug PTX within the cell [ 5 , 6 , 27 ]. DPSCs showed a great capacity to uptake PTX.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the mechanism and behavior of MSCs and tracking them to check their efficacy in cancer treatment are essential for assessing their future clinical potential. Confocal Raman microscopy is the method able to track living cells [ 5 , 6 , 27 – 29 ] without a need for labeling. The laser beam being focused down to a small spot on the specimen leads to high spatial resolution and unique compositional sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…This method allows cells’ analysis according to different vibrational spectra owing to differences in the biochemical composition of the molecules. The drug’s specific Raman signature enables its precise detection in the cell [ 5 , 6 , 27 , 28 ]. Here we used MSCs of the dental pulp to assess for the first time whether they can be a promising delivery vehicle for PTX.…”

Section: Introductionmentioning

confidence: 99%

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Dental pulp stem cells used to deliver the anticancer drug paclitaxel

et al. 2018

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BackgroundUnderstanding stem cell behavior as a delivery tool in cancer therapy is essential for evaluating their future clinical potential. Previous in-vivo studies proved the use of mesenchymal stem cells (MSCs) for local delivery of the commonest anticancer drug, paclitaxel (PTX). Dental pulp is a relatively abundant noninvasive source of MSCs. We assess dental pulp stem cells (DPSCs), for the first time, as anticancer drug carriers. Confocal Raman microscopy is a unique tool to trace drug and cell viability without labeling.MethodsDrug uptake and cell apoptosis are identified through confocal Raman microscope. We traced translocation of cytochrome c enzyme from the mitochondria, as a biomarker for apoptosis, after testing both cancer and stem cells. The viability of stem cells was checked by means of confocal Raman microscope and by cytotoxicity assays.ResultsIn this study, we prove that DPSCs can be loaded in vitro with the anticancerous drug without affecting their viability, which is later released in the culture medium of breast cancer cells (MCF-7 cells) in a time-dependent fashion. The induced cytotoxic damage in MCF-7 cells was observed consequently after PTX release by DPSCs. Additionally, quantitative Raman images of intracellular drug uptake in DPSCs and MCF-7 cells were obtained. Cytotoxic assays prove the DPSCs to be more resistant to PTX as compared to bone marrow-derived MSCs, provided similar conditions.ConclusionsApplications of dental stem cells for targeted treatment of cancer could be a revolution to reduce morbidity due to chemotherapy, and to increase the efficacy of systemic cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dental pulp stem cells used to deliver the anticancer drug paclitaxel

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“…Salehi and co‐workers employed confocal Raman microscopy, a noninvasive, label‐free and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map to trace titanium dioxide (TiO 2 ) nanoparticles in living MCF‐7 and TERT cells. They demonstrate that confocal Raman microscopy allows one to follow the penetration of TiO 2 particles into cells and to monitor the apoptotic status of the penetrated cells …”

Section: Biosciencesmentioning

confidence: 99%

Recent advances in linear and non‐linear Raman spectroscopy. Part IX

Nafie

2015

J Raman Spectroscopy

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Coverage is also provided for topics from the conference ECONOS 2015 held in April in Leuven, Belgium. Finally, papers published in JRS in 2014 are highlighted and arranged by topics at the frontier of Raman spectroscopy. Taken from these various viewpoints, it is clear that Raman spectroscopy continues to be a rapidly expanding field that provides sensitive photonic information of matter at the molecular level in an ever-widening arena of novel applications.

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“…Confocal Raman microscopy with its high spatial resolution and unique compositional sensitivity is used as a unique label-free tool to trace drugs and nanoparticles within cells. [27][28][29][30][31] The cells and their components are analysed via their vibrational spectra owing to differences in their biochemical compositions. The Raman nanoparticle signature therefore enables precise nanoparticle detection in the cell without any labeling.…”

Section: Introductionmentioning

confidence: 99%

<p>Assessing Cobalt Metal Nanoparticles Uptake by Cancer Cells Using Live Raman Spectroscopy</p>

Rauwel¹,

Al-Arag²,

Salehi³

et al. 2020

IJN

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Nanotechnology applied to cancer treatment is a growing area of research in nanomedicine with magnetic nanoparticle-mediated anti-cancer drug delivery systems offering least possible side effects. To that end, both structural and chemical properties of commercial cobalt metal nanoparticles were studied using label-free confocal Raman spectroscopy. Materials and Methods: Crystal structure and morphology of cobalt nanoparticles were studied by XRD and TEM. Magnetic properties were studied with SQUID and PPMS. Confocal Raman microscopy has high spatial resolution and compositional sensitivity. It, therefore, serves as a label-free tool to trace nanoparticles within cells and investigate the interaction between coating-free cobalt metal nanoparticles and cancer cells. The toxicity of cobalt nanoparticles against human cells was assessed by MTT assay. Results: Superparamagnetic Co metal nanoparticle uptake by MCF7 and HCT116 cancer cells and DPSC mesenchymal stem cells was investigated by confocal Raman microscopy. The Raman nanoparticle signature also allowed accurate detection of the nanoparticle within the cell without labelling. A rapid uptake of the cobalt nanoparticles followed by rapid apoptosis was observed. Their low cytotoxicity, assessed by means of MTT assay against human embryonic kidney (HEK) cells, makes them promising candidates for the development of targeted therapies. Moreover, under a laser irradiation of 20mW with a wavelength of 532nm, it is possible to bring about local heating leading to combustion of the cobalt metal nanoparticles within cells, whereupon opening new routes for cancer phototherapy. Conclusion: Label-free confocal Raman spectroscopy enables accurately localizing the Co metal nanoparticles in cellular environments. The interaction between the surfactant-free cobalt metal nanoparticles and cancer cells was investigated. The facile endocytosis in cancer cells shows that these nanoparticles have potential in engendering their apoptosis. This preliminary study demonstrates the feasibility and relevance of cobalt nanomaterials for applications in nanomedicine such as phototherapy, hyperthermia or stem cell delivery.

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Confocal Raman spectroscopy to monitor intracellular penetration of TiO₂ nanoparticles

Cited by 12 publications

References 53 publications

Dental pulp stem cells used to deliver the anticancer drug paclitaxel

Dental pulp stem cells used to deliver the anticancer drug paclitaxel

Recent advances in linear and non‐linear Raman spectroscopy. Part IX

<p>Assessing Cobalt Metal Nanoparticles Uptake by Cancer Cells Using Live Raman Spectroscopy</p>

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Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles

Cited by 12 publications

References 53 publications

Dental pulp stem cells used to deliver the anticancer drug paclitaxel

Dental pulp stem cells used to deliver the anticancer drug paclitaxel

Recent advances in linear and non‐linear Raman spectroscopy. Part IX

<p>Assessing Cobalt Metal Nanoparticles Uptake by Cancer Cells Using Live Raman Spectroscopy</p>

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Confocal Raman spectroscopy to monitor intracellular penetration of TiO₂ nanoparticles