Erkek Ratlarda Maclura pomifera Ekstraktının Sisplatin ile Oluşturulmuş Üreme Sistemi Hasarı Üzerine Etkisi

Aksu, Emrah Hicazi; Akman, Orhan; Özkaraca, Mustafa; Ömür, Ali Doğan; Uçar, Ömer

doi:10.9775/kvfd.2014.12662

Cited by 11 publications

(9 citation statements)

References 22 publications

(39 reference statements)

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“…This situation can be explained by that MTX damages cell membrane integrity by disturbing lipids and proteins within the sperm membrane. It was determined in previous studies that like MTX, Cisplatin causes a marked decrease in sperm density and motility [32][33][34] .…”

Section: Discussionmentioning

confidence: 99%

Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

Belhan¹,

Özkaraca²,

Kandemir³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleBelhan S, Özkaraca M, Kandemir FM, Gülyüz F, Yıldırım S, Ömür AD, Yener Z: Effectiveness of hesperidin on methotrexate-induced testicular toxicity in rats. Kafkas Univ Vet Fak Derg, 23 (5): 789-796, 2017789-796, . DOI: 10.9775/kvfd.2017 AbstractThe aim of this study was to investigate the effect of hesperidin on male reproductive system in rats to which methotrexate (MTX) was administered. In the study, 28 male Wistar albino rats at the age of 8 weeks and had 250-300 g of live weight were used. Four experimental groups were formed; Group 1 (n=7): The control group, only feed and water were given. Group 2 (n=7): MTX group, a single dose of 20 mg/ kg of i.p. MTX was administered. Group 3 (n=7): Hesperidin group, 200 mg/kg of hesperidin was administered by gavage for 7 days. Group 4: MTX + hesperidin group (n=7): Following administration of a single dose of 20 mg/kg i.p. MTX , 200 mg/kg of Hesperidin was administered by oral gavage for 7 days. At the end of the experiment, rats were decapitated and biochemical, histopathological and spermatological parameters were examined. It was observed that in the MTX group, sperm motility and density, the enzymes CAT, GPx and SOD and GSH level decreased, TNF-alpha and IL-1 Beta, as well as MDA, levels were increased, regular structure of spermatogenic cells was impaired, and seminiferous tubules became necrotic and degenerative. It was determined that spermatological parameters improved and, necrotic and degenerative changes diminished by the administration of MTX+hesperidin. These outcomes indicated that hesperidin had a protective effect on destructive effects of MTX in rat testiclesv. Keywords: Male rat, Hesperidin, Methotrexate, Sperm, Reproductive system Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi ÖzetBu çalışmanın amacı, metoraksat (MTX) uygulanan ratlarda hesperidinin erkek üreme sistemi üzerindeki etkisini araştırmaktı. Çalışmada 8 haftalık yaşta, 250-300 g canlı ağırlığa sahip 28 erkek Wistar albino cinsi rat kullanıldı. Dört deney grubu oluşturuldu; Grup 1 (n=7): kontrol grubu, sadece yem ve su verildi. Grup 2 (n=7): MTX grubu, 20 mg/kg MTX i.p. tek doz uygulandı. Grup 3 (n=7): Hesperidin grubu, 200 mg/kg hesperidin 7 gün süre ile ağızdan sonda yardımıyla verildi. Grup 4 (n=7): MTX + hesperidin grubu, 20 mg/kg MTX i.p tek doz uygulamasını takiben 200 mg/kg hesperidin 7 gün süre ile ağızdan sonda yardımıyla verildi. Deney sonunda ratlar dekapite edilerek biyokimyasal, histopatolojik ve spermatolojik parametreler incelendi. MTX grubunda sperm motilitesi ve yoğunluğunun, CAT, GPx ve SOD enzimlerinin ve GSH düzeyinin düştüğü, TNF-alpha ve IL-1 Beta ile MDA düzeyinin arttığı, spermatojenik hücrelerin düzenli yapısının bozulduğu, seminifer tubullerin nekrotik ve dejeneratif bir hal aldığı gözlendi. MTX + hesperidin uygulaması ile spermatolojik parametrelerin düzeldiği, nekrotik ve dejeneratif değişikliklerin azaldığı tespit edildi. Bu sonuçlar hesperidinin rat testislerinde MTX tarafından oluşan yıkıcı etkiler üze...

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Section: Discussionmentioning

confidence: 99%

Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

Belhan¹,

Özkaraca²,

Kandemir³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…Semen density was determined following the method described in our previous study . Briefly, 10‐µL of a semen sample was dropped in 990 µL PS and thus diluted to 1 of 100 in a centrifuge tube.…”

Section: Methodsmentioning

confidence: 99%

Improvement in colistin‐induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin

Aksu

Kandemir

Küçükler

et al. 2018

J Biochem & Molecular Tox

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This study aimed to investigate the effect of chrysin on colistin‐induced reproductive toxicity. Twenty‐eight adult male Sprague‐Dawley rats were divided into four groups of seven rats each. Group I received physiological saline for 7 days. Group II received 50 mg/kg/day chrysin for 7 days. Group III received a total dose of 73 mg/kg colistin for 7 days. Group IV received 50 mg/kg/day chrysin by an oral gavage after the colistin treatment. Colistin causes an increase in oxidative stress (OS) in the testis. Chrysin treatment significantly decreased the OS in the chrysin + colistin group compared with the colistin group. The highest caspase‐3 and LC3B expression levels were found in the colistin group and these levels were statistically lower in the chrysin + colistin group. Colistin treatment caused a decrease in sperm motility and an increase in sperm abnormality. Chrysin treatment mitigated these side effects significantly. In conclusion, chrysin treatment can be beneficial against colistin‐induced reproductive toxicity.

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“…It is suggested that DOX treatment increases the production of quinone, a free radical that causes cytotoxicity . Reactive oxygen species (ROS) are required for some kind of physiological events such as sperm maturation, capacitation of sperm cells, and sperm‐oocyte interactions in physiological limits . Increased levels of ROS have negative effects on sperm cells.…”

Section: Introductionmentioning

confidence: 99%

Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats

Aksu

Kandemir

Yıldırım

et al. 2019

J Biochem & Molecular Tox

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This study aimed to investigate the effect of curcumin (CUR) on doxorubicin (DOX)‐induced testicular damage in male rats. Thirty‐five adult male Wistar rats were used. Control group was received saline for 7 days. CUR group received CUR for 7 days. DOX group received single dose DOX on the 5th day. DOX+ CUR‐100 group received 100 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX + CUR‐200 group received 200 mg/kg/day CUR for 7 days and DOX injection on the 5th day. DOX treatment decreased in sperm motility rate, live sperm percentages, cellular antioxidants, and increased malondialdehyde (MDA) levels, necrosis, degenerations, and slimming in seminiferous tubules, and DNA damages in testes by inducing oxidative stress. CUR treatment mitigated significantly these side effects when compared with DOX group in a dose‐dependent manner. In conclusion, CUR treatment can be used in the mitigation of DOX‐induced testicular toxicity.

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Erkek Ratlarda Maclura pomifera Ekstraktının Sisplatin ile Oluşturulmuş Üreme Sistemi Hasarı Üzerine Etkisi

Cited by 11 publications

References 22 publications

Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

Improvement in colistin‐induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin

Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats

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