Ergothioneine Contents in Fruiting Bodies and Their Enhancement in Mycelial Cultures by the Addition of Methionine

Lee, Wi Young; Park, Eung‐Jun; Ahn, Jin Kwon; Ka, Kang-Hyeon

doi:10.4489/myco.2009.37.1.043

Cited by 15 publications

(10 citation statements)

References 33 publications

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“…The content of ET (retention time at 3.00) in the He1 WT sporophore was 0.34 mg/g (d.w.), measured by comparison with ET calibration curve. This value is slightly smaller compared to data reported in literature, i.e., 0.96 mg/g [54] and 1.12 mg/g [55].…”

Section: Array Of Metabolites In Hericium Erinaceus Extractcontrasting

confidence: 73%

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

Roda

Priori

Ratto

et al. 2021

IJMS

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Frailty is a geriatric syndrome associated with both locomotor and cognitive decline, typically linked to chronic systemic inflammation, i.e., inflammaging. In the current study, we investigated the effect of a two-month oral supplementation with standardized extracts of H. erinaceus, containing a known amount of Erinacine A, Hericenone C, Hericenone D, and L-ergothioneine, on locomotor frailty and cerebellum of aged mice. Locomotor performances were monitored comparing healthy aging and frail mice. Cerebellar volume and cytoarchitecture, together with inflammatory and oxidative stress pathways, were assessed focusing on senescent frail animals. H. erinaceus partially recovered the aged-related decline of locomotor performances. Histopathological analyses paralleled by immunocytochemical evaluation of specific molecules strengthened the neuroprotective role of H. erinaceus able to ameliorate cerebellar alterations, i.e., milder volume reduction, slighter molecular layer thickness decrease and minor percentage of shrunken Purkinje neurons, also diminishing inflammation and oxidative stress in frail mice while increasing a key longevity regulator and a neuroprotective molecule. Thus, our present findings demonstrated the efficacy of a non-pharmacological approach, based on the dietary supplementation using H. erinaceus extract, which represent a promising adjuvant therapy to be associated with conventional geriatric treatments.

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Section: Array Of Metabolites In Hericium Erinaceus Extractcontrasting

confidence: 73%

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

Roda

Priori

Ratto

et al. 2021

IJMS

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“…Lee et al [108] determined the level of ergothioneine in the mushroom species growing in South Korea. The highest content of this amino acid was observed in L. nuda (5.54 mg/g d.w.), Hygrophorus russula (4.98 mg/g d.w.), and C. cibarius (4.09 mg/g d.w.).…”

Section: Ergothioneinementioning

confidence: 99%

“…The highest content of this amino acid was observed in L. nuda (5.54 mg/g d.w.), Hygrophorus russula (4.98 mg/g d.w.), and C. cibarius (4.09 mg/g d.w.). The content of ergothioneine in B. edulis was not studied; however, in another representative of this species (Boletus auripes), the level of ergothioneine was calculated as 2.4 mg/g d.w. [108].…”

Section: Ergothioneinementioning

confidence: 99%

Culinary–medicinal mushrooms: a review of organic compounds and bioelements with antioxidant activity

Podkowa

Kryczyk-Poprawa

Opoka

et al. 2020

Eur Food Res Technol

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There are about 3000 species of mushrooms, which have a high amount of substances that are beneficial to human health, such as antioxidants. It is well known that oxidative stress plays an important role in the etiopathogenesis of many diseases, including cancer, cardiovascular disorders, and diseases of the central nervous system. One way to prevent homeostasis disorders that occur as a result of excessive production of pro-oxidative substances is to include the ingredients having antioxidant properties in the diet. Several compounds, such as those with phenolic and indole derivatives as well as carotenoids and some vitamins, exhibit antioxidant activity. These substances are present in many foods, including mushrooms. In addition, they have certain unique compounds that are not found in other sources (e.g., norbadione A). The present work discusses selected ingredients exhibiting antioxidant activity, which are found in various species of mushrooms as wells as describes the content of these compounds in the extracts obtained from mushrooms using artificial digestive juice.

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“…And seventeen times higher than the yields reported by Jang et al 23 who used Shiitake submerged cultures supplemented with various nitrogen sources. Unexpectedly, the isolates used in this experiment contained eight times more ergothioneine than the body fruiting of the popular Ganoderma lucidum 21 .…”

Section: Ergothioneine Assaymentioning

confidence: 87%

“…The secondary metabolite ergothioneine (2-mercaptohistidine trimethylbetaine = ERG) is one of the main antioxidant substances in Shiitake, with potent antioxidant properties for cells and DNA 18 , and is antinflamatory 19 . Different authors evaluated ergothioneine contents in many mushrooms 20,21 . Additionally, other authors showed interest in establishing adequate cultivation parameters, to enhance the ergothioneine production, in Shiitake mycelium 22 and fruitbodies 23 .…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential

Durán-Rivera

Rojas-Rodas

Silva-López

et al. 2020

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The Shitake mushroom (Lentinula edodes) is the second most-consumed mushroom in the world; in Colombia, it is cultivated and commercialized on a small scale in some supermarkets. Little is known about the precedence, nutritional and medicinal properties of Shiitake produced in Colombia. In this study, four shiitake isolates were grown in Colombia (LEUCO1, LEUCO2, LEUCO3, and LEUCO4) were sequenced in their ITS genes and evaluated for the production of three medicinal metabolites, eritadenine, ergotioneine and β-glucans (1,3-1, 6), using submerged culture. Genetic analysis revealed that all the isolates were close and related to the Japanese strain Cr62. LEUCO1 and LEUCO2 showed a distance of 0.000, as well as LEUCO3 and LEUCO4. All four isolates produced erythadenin in a range of 26.3-8.6 mg / L, with the best performance of LEUCO1 at 26.3 mg / L (p <0.05). Ergotioneine was produced with similar statistical yields in all the isolates with an average of 0.7 mg / g of dry weight biomass (DW). Β-glucans (1.3-1.6) were produced with yields of 5.6 - 3.8% of DW biomass, with the best values for LEUCO2 and the lowest for LEUCO4 (p <0.05). In conclusion, we identified low genetic diversity in the four isolates, corresponding to two haplotypes with minimal genetic difference between them, related to the Japanese strain Cr62, indicating that Colombian farmers cultivate almost the same strains of shitake. Secondary metabolites, eritadenine, β-glucans and ergotioneine were found in promising yields useful for the pharmaceutical and food industries. More studies should be conducted to improve the yield of shitake metabolites through new growing conditions for industrial production and to find metabolic pathways and related genes.

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Ergothioneine Contents in Fruiting Bodies and Their Enhancement in Mycelial Cultures by the Addition of Methionine

Cited by 15 publications

References 33 publications

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

Culinary–medicinal mushrooms: a review of organic compounds and bioelements with antioxidant activity

Molecular identification of Shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential

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