Ergot infection in winter rye hybrids shows differential contribution of male and female genotypes and environment

Kodisch, Anna; Wilde, Peer; Schmiedchen, Brigitta; Fromme, Franz-Joachim; Rodemann, Bernd; Tratwal, Anna; Oberforster, M.; Wieser, Franz; Schiemann, A.; Jørgensen, Lise Nistrup; Miedaner, Thomas

doi:10.1007/s10681-020-02600-2

Cited by 18 publications

(22 citation statements)

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“…Because no fungicides against ergot are registered on the EU market [25,26], it is of utmost importance to reduce ergot sclerotia in the rye harvest by breeding. It was shown previously that cultivars with a high pollen shedding lead always to a lower ergot contamination [27,28], thus confirming the tight correlations between anther score and ergot severity found here among 15 single crosses.…”

Section: Discussionsupporting

confidence: 90%

“…In this earlier study, the main differences among EA contents came from isolate and environmental factors. Screening approaches that are fast, cheap, and easy to handle were found to be not yet convincing because of a poor relationship between ELISA and HPLC results in recent calibration studies [28,35]. The correlation of ELISA and HPLC found in this study was moderate, which could also be caused by the higher range for ergot reaction of the genotypes here.…”

Section: Discussionmentioning

confidence: 59%

“…28 25.5 N 9 • 18 17.9 E), Braunschweig (BRS; 52 • 16 33.4 N 10 • 34 09.3 E), Wohlde (WOH; 52 • 48 48.7 N 9 • 59 53.1 E), Petkus (PET; 51 • 59 14 N 13 • 21 22 E) in Germany, Kościelna Wieś (KOS; 51 • 46 28.7 N 18 • 00 58.0 E), and Zybiszów (ZYB; 51 • 03 51.9 N 16 • 54 45.4 E) in Poland. Data of BRS 2018 were lacking because of missing infections.…”

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confidence: 99%

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Ergot Alkaloid Contents in Hybrid Rye are Reduced by Breeding

et al. 2021

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Contamination by ergot caused by the phytopathogenic fungus Claviceps purpurea is a constant threat to the whole rye value chain. Ergot alkaloids (EA) produced within the fungal sclerotia are toxic for humans and animals and are subjected to strict regulations in human food. Our main objective was to analyze whether less susceptible rye cultivars with a lower content of sclerotia also contain fewer ergot alkaloids (EA). We analyzed 15 factorial single crosses in multi-environmental trials with artificial inoculation for their ergot severity, the content of twelve EAs by HPLC, and the total ergot content by ELISA. The genotypes displayed a wide range of pollen shedding from fully sterile to fully fertile, of ergot severity expressed as percentage of sclerotia relative to the harvest (0.22–11.47%), and of EA contents when analyzed by HPLC (0.57–45.27 mg/kg. Entry-mean heritabilities were high throughout (0.87–0.98). The factorial analysis yielded a preponderance of male general combining ability (GCA) variances, the estimates for the females were smaller, although significant. EA contents measured by ELISA were, on average, seven times larger. The correlation between ergot severity and EA contents determined by HPLC was r = 0.98 (p ≤ 0.01) and only somewhat lower when analyzed by ELISA. In conclusion, less ergot prone rye genotypes also support lower EA contents.

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confidence: 90%

Section: Discussionmentioning

confidence: 59%

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Ergot Alkaloid Contents in Hybrid Rye are Reduced by Breeding

et al. 2021

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“…Restoration of male fertility in hybrids derived from the predominant P-type cytoplasm is frequently incomplete and highly environmentally unstable [9,[19][20][21]. In addition to a potential loss in grain yield, partial pollination renders the cultivar susceptible to fungal infection by ergot (Claviceps purpurea (Fr.)…”

Section: Introductionmentioning

confidence: 99%

Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye (Secale cereale L.)

Vendelbo

Mahmood²,

Sarup³

et al. 2021

IJMS

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Efficient and stable restoration of male fertility (Rf) is a prerequisite for large-scale hybrid seed production but remains an inherent issue in the predominant fertility control system of rye (Secale cereale L.). The ‘Gülzow’ (G)-type cytoplasmic male sterility (CMS) system in hybrid rye breeding exhibits a superior Rf. While having received little scientific attention, one major G-type Rf gene has been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosomes. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using recent advents in rye genomic resources. This includes: (I) genome-wide association studies (GWAS) on G-type germplasm; (II) GWAS on a biparental mapping population; and (III) an RNA sequence study to investigate the expression of genes residing in Rf-associated regions in G-type rye hybrids. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome belonging to the mitochondrial transcription termination factor gene family. We provisionally denote the identified novel Rf gene on 3RL RfNOS1. The discovery made in this study is distinct from known P- and C-type systems in rye as well as recognized CMS systems in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). We believe this study constitutes a stepping stone towards understanding the restoration of male fertility in the G-type CMS system and potential resources for addressing the inherent issues of the P-type system.

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“…Restoration of male-fertility in hybrids derived from the predominant P-type cytoplasm is frequently partial and highly environmental unstable 9,[19][20][21] . In addition to a potential loss in grain yield, impartial pollination renders the cultivar susceptible to fungal infection of ergot (Claviceps purpurea (Fr.)…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Genetic architecture of male fertility restoration in a hybrid breeding system of rye (Secale cereale L.)

Vendelbo¹,

Mahmood²,

Sarup³

et al. 2021

Preprint

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The ‘Gülzow’ (G) type cytoplasmic male sterility (CMS) system in hybrid rye (Secale cereale L.) breeding exhibits a strong and environmentally stable restoration of male fertility (Rf). While having received little scientific attention, three G-type Rf genes had been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosome. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using a palette of complementing forward and reverse genetic analysis. This includes (i) genome wide association studies (GWAS) on a G-type germplasm, (ii) GWAS on a biparental mapping population, (iii) in silico identification of Rf-like pentatricopeptide repeat (RFL-PPR) genes and their expressed in G-type rye hybrids, and (iv) mining patterns in linkage disequilibrium. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome. In the in silico analysis, we identified 22 RFL-PPR of which 15 were expressed in the transcriptome of G-type hybrids. Our findings provides a novel insight into the underlying genetics of male fertility restoration in a G-type CMS system in rye. The discovery made in this study is distinct to known P- and C-type systems in rye in addition to known CMS systems in barley and wheat. This study constitutes a steppingstone towards understanding the restoration of male fertility in G-type CMS system and a potential resources for addressing the inherent issues of the P-type system.

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Ergot infection in winter rye hybrids shows differential contribution of male and female genotypes and environment

Cited by 18 publications

References 19 publications

Ergot Alkaloid Contents in Hybrid Rye are Reduced by Breeding

Ergot Alkaloid Contents in Hybrid Rye are Reduced by Breeding

Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye (Secale cereale L.)

Genetic architecture of male fertility restoration in a hybrid breeding system of rye (Secale cereale L.)

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