Erg6 gene is essential for stress adaptation in<i>Kluyveromyces lactis</i>

Konecna, Alexandra; Hervay, Nora Tóth; Bencova, Alexandra; Morvová, Marcela; Šikurová, L; Jančíková, Iva; Gášková, Dana; Gbelská, Yvetta

doi:10.1093/femsle/fny265

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…Klupc2Δ mutant strain exhibited mostly similar phenotype to S. cerevisiae upc2Δ mutant but different susceptibility to calcofluor white and tunicamycin. The increased tolerance of K. lactis upc2Δ mutant to calcofluor white and tunicamycin was comparable with the recently described phenotype of K. lactis erg6Δ mutant (Konecna, Toth Hervay, & Bencova, 2018; Konecna, Toth Hervay, Valachovic, & Gbelska, 2016). Garcia‐Rodriguez, Duran, and Roncero (2000) reported that the sensitivity of yeast cells to calcofluor white depends on the functionality of the HOG pathway.…”

Section: Discussionsupporting

confidence: 87%

UPC2 gene deletion modifies sterol homeostasis and susceptibility to metabolic inhibitors in Kluyveromyces lactis

Hervay

Bencova

Valachovič

et al. 2020

Yeast

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Kluyveromyces lactis Upc2p is an ortholog of Upc2p/Ecm22p transcription factors involved in regulation of sterol import and sterol homeostasis in Saccharomyces cerevisiae. In this work, we investigated the role of Upc2p in K. lactis. The absence of KlUpc2p significantly reduced the tolerance of mutant cells to antifungal azoles and Li + cations. Reduced expression of genes from the late ergosterol pathway results in a decreased ergosterol content and altered plasma membrane-associated functions in Klupc2 mutant cells-the plasma membrane is hyperpolarized, and its fluidity is reduced. KlUpc2p contributes to transcriptional upregulation of KlENA1, KlPMA1 and KlYAP1 under azole stress. Our study demonstrates that KlUpc2p is involved in the regulation of ergosterol homeostasis in K. lactis. The analysis of KlPMA1 and KlPDR12 transcripts in wild-type and Klupc2Δ mutant strains showed that KlUpc2p acts as an activator or as a repressor depending upon its target.

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Section: Discussionsupporting

confidence: 87%

UPC2 gene deletion modifies sterol homeostasis and susceptibility to metabolic inhibitors in Kluyveromyces lactis

Hervay

Bencova

Valachovič

et al. 2020

Yeast

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“…The lack of erg6 in Kluyveromyces lactis caused reduced ergosterol content in the cells and an impairment in the stress adaptation of the fungus ( 39 ). In our study, CW, CR, and SDS were used to induce cell wall and membrane stresses.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Sterol 24-C-Methyltransferase Genes Reveals a Network of Alternative Sterol Biosynthetic Pathways in Mucor lusitanicus

et al. 2023

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“…It remains unclear specifically why erg6 is strongly up-regulated upon azole perturbation, although this was also observed in previous studies [ 36 , 83 , 84 ]. This enzyme is a potential antifungal target, as Erg6 catalyzes a reaction that is not present in the cholesterol biosynthesis pathway [ 85 ].…”

Section: Discussionmentioning

confidence: 99%

The Transcriptome Response to Azole Compounds in Aspergillus fumigatus Shows Differential Gene Expression across Pathways Essential for Azole Resistance and Cell Survival

Hokken,

Coolen,

Steenbreker

et al. 2023

JoF

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The opportunistic pathogen Aspergillus fumigatus is found on all continents and thrives in soil and agricultural environments. Its ability to readily adapt to novel environments and to produce billions of spores led to the spread of azole-resistant A. fumigatus across the globe, posing a threat to many immunocompromised patients, including critically ill patients with severe influenza or COVID-19. In our study, we sought to compare the adaptational response to azoles from A. fumigatus isolates that differ in azole susceptibility and genetic background. To gain more insight into how short-term adaptation to stressful azole compounds is managed through gene expression, we conducted an RNA-sequencing study on the response of A. fumigatus to itraconazole and the newest clinically approved azole, isavuconazole. We observed many similarities in ergosterol biosynthesis up-regulation across isolates, with the exception of the pan-azole-resistant isolate, which showed very little differential regulation in comparison to other isolates. Additionally, we found differential regulation of membrane efflux transporters, secondary metabolites, iron metabolism, and various stress response and cell signaling mechanisms.

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Erg6 gene is essential for stress adaptation inKluyveromyces lactis

Cited by 7 publications

References 40 publications

UPC2 gene deletion modifies sterol homeostasis and susceptibility to metabolic inhibitors in Kluyveromyces lactis

UPC2 gene deletion modifies sterol homeostasis and susceptibility to metabolic inhibitors in Kluyveromyces lactis

Characterization of the Sterol 24-C-Methyltransferase Genes Reveals a Network of Alternative Sterol Biosynthetic Pathways in Mucor lusitanicus

The Transcriptome Response to Azole Compounds in Aspergillus fumigatus Shows Differential Gene Expression across Pathways Essential for Azole Resistance and Cell Survival

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