Erdosteine ameliorates PTZ-induced oxidative stress in mice seizure model

İlhan, Atilla; Aladağ, Mehmet Arif; Koçer, Abdülkadir; Bölük, Ayhan; Gürel, Ahmet; Armutçu, Ferah

doi:10.1016/j.brainresbull.2005.02.027

Cited by 45 publications

(22 citation statements)

References 23 publications

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“…In the present study, PTZ-induced seizures caused a marked decrease in GSH levels and a significant increase in MDA levels compared to the normal group. In agreement with our results, the PTZ-induced oxidative stress has been reported in previous studies [44, 45]. However, these changes were ameliorated by Rb1.…”

Section: Discussionsupporting

confidence: 83%

Ginsenoside Rb1 Protects the Brain from Damage Induced by Epileptic Seizure via Nrf2/ARE Signaling

Shi

Wang

Teng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ginsenoside Rb1 (Rb1) has been reported to have varieties of neuroprotective effects. This study aimed to evaluate the effects of Rb1 on pentylenetetrazol (PTZ)-induced rat brain injury and Mg²⁺ free-induced neuron injury and analyzed the detailed molecular mechanisms in vivo and in vitro. Methods: Seizure duration and latency were measured in epilepsy kindled rat. The cognitive impairment was assessed by Morris water maze (MWM) test. Oxidative stress parameters, malondialdehyde (MDA) and glutathione (GSH) were measured by the 2-thiobarbituric acid methods and the DTNB-GSSG reductase recycling methods. Neuronal damage was assessed by hematoxylin and eosin (H&E) and Nissl staining. Neuronal apoptosis was measured by Annexin V-FITC and propidium iodide (PI) staining. Immunohistochemistry and immunofluorescence staining were performed to evaluate Nrf2 and HO-1 expressions. Expression of Nrf2, HO-1, Bcl-2, iNOS and LC3 were evaluated by western blot. Results: The PTZ-injured rats presented longer seizure duration and shorter seizure latency. Rb1 ameliorated these effects, as well as the cognitive deficits caused by PTZ exposure. Besides, Rb1 dose-dependently increased GSH levels, decreased MDA levels and alleviated neuronal damage in PTZ-treated rats. In vitro, Rb1 increased cell viability and decreased neuronal apoptosis in a dose-dependent manner under Mg²⁺ free condition. Moreover, in vivo and in vitro, Rb1 enhanced both the Nrf2 and HO-1 expressions. Furthermore, upregulation of the expression of Bcl-2 and downregulation of the expression of iNOS and LC3 were observed. However, knockdown of Nrf2 adversely affected the protective effects of Rb1 in epileptic hippocampal neurons. Conclusion: Rb1 conferred neuroprotective effects against PTZ-induced brain damage and Mg²⁺ free-induced neuron injury by activating Nrf2/ARE signaling.

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Section: Discussionsupporting

confidence: 83%

Ginsenoside Rb1 Protects the Brain from Damage Induced by Epileptic Seizure via Nrf2/ARE Signaling

Shi

Wang

Teng

et al. 2018

Cell Physiol Biochem

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“…The flask contents were shaken for 5 min and centrifuged at 2000 rpm for 15 min. A clear supernatant was collected from centrifugation (Atilla Ilhan et al, 2005). The optical density of the supernatant was read against a blank in Shimadzu UV3600 spectrophotometer.…”

Section: Effect On Brain Lipid Peroxidationmentioning

confidence: 99%

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Asuntha¹,

Prasannaraju²,

Sujatha³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Avaliação do efeito do extrato etanólico da Tephrosia purpurea (L.) Pers., Fabaceae, sobre o status epilepticus induzido por lítio-pilocarpina e estresse oxidativo, em ratos Wistar". Tephrosia purpurea (L.) Pers., Fabaceae, é conhecida pelo seu uso no controle e tratamento de uma variedade de distúrbios epilépticos no sistema indiano de medicina. O presente estudo pretende avaliar de forma sistemática T. purpurea e verificar essa alegação. ABSTRACT: Tephrosia purpurea (L.) Pers., Fabaceae, is claimed to be of use in the control and treatment of a variety of epileptic disorders in Indian system of medicine. The present study plans to systematically evaluate T. purpurea and to verify this claim. Status epilepticus was induced in male albino rats of Wistar strain by administration of pilocarpine (30 mg/kg, i.p.) 24 h after lithium chloride (3 mEq/kg, i.p.). Different doses of the extract of T. purpurea were administered orally one hour before the injection of pilocarpine. The severity of status epilepticus was observed and recorded every 15 min till 90 min and thereafter every 30 min till 180 min, using the scoring system. The in vivo lipid peroxidation of rat brain tissue was measured. The in vitro NO free radical scavenging activity of plant extract was assessed. The interaction between plant extract and 2-diphenyl-2-picryl hydrazyl (DPPH) was also observed for in vitro free radical scavenging activity. The severity of status epilepticus was reduced with the administration of ethanol extract of T. purpurea. Ethanol extract of the plant exhibited both in vivo and in vitro antioxidant activity. The ethanolic extract of T. purpurea was found to be useful to control lithium-pilocarpine induced status epilepticus in albino rats of Wistar strain.

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“…In the present study, lipid peroxidation and nitrite levels increased after status epilepticus. Lipid peroxidation is an index of irreversible neuronal damage of cell membrane phospholipid and has been suggested as a possible mechanism of epileptic activity [24]. A steady-state balance between the production of nitric oxide and metabolites and their destruction by antioxidant systems is present at normal conditions.…”

Section: Discussionmentioning

confidence: 99%

Effect of Ethanol Extract of <i>Indigofera tinctoria</i> Linn (Fabaceae) on Lithium/Pilocarpine-Induced Status Epilepticus and Oxidative Stress in Wistar Rats

Asuntha¹,

Prasannaraju²,

Prasad³

2010

Trop. J. Pharm Res

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Erdosteine ameliorates PTZ-induced oxidative stress in mice seizure model

Cited by 45 publications

References 23 publications

Ginsenoside Rb1 Protects the Brain from Damage Induced by Epileptic Seizure via Nrf2/ARE Signaling

Ginsenoside Rb1 Protects the Brain from Damage Induced by Epileptic Seizure via Nrf2/ARE Signaling

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Effect of Ethanol Extract of <i>Indigofera tinctoria</i> Linn (Fabaceae) on Lithium/Pilocarpine-Induced Status Epilepticus and Oxidative Stress in Wistar Rats

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